Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0051 and Shew_0052 are separated by 95 nucleotides Shew_0052 and Shew_0053 are separated by 23 nucleotides
Shew_0051: Shew_0051 - cyclic nucleotide-binding protein (RefSeq), at 61,803 to 63,692
_0051
Shew_0052: Shew_0052 - DNA binding domain-containing protein (RefSeq), at 63,788 to 64,693
_0052
Shew_0053: Shew_0053 - formate dehydrogenase, subunit FdhD (RefSeq), at 64,717 to 65,562
_0053
Position (kb) 63
64
65 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 62.993 kb on + strand, within Shew_0051 at 63.001 kb on - strand, within Shew_0051 at 63.001 kb on - strand, within Shew_0051 at 63.001 kb on - strand, within Shew_0051 at 63.141 kb on - strand, within Shew_0051 at 63.293 kb on + strand, within Shew_0051 at 63.301 kb on - strand, within Shew_0051 at 63.301 kb on - strand, within Shew_0051 at 63.303 kb on + strand, within Shew_0051 at 63.333 kb on + strand, within Shew_0051 at 63.426 kb on - strand, within Shew_0051 at 63.474 kb on + strand, within Shew_0051 at 63.474 kb on + strand, within Shew_0051 at 63.526 kb on + strand at 63.806 kb on - strand at 63.834 kb on + strand at 63.836 kb on + strand at 64.081 kb on + strand, within Shew_0052 at 64.089 kb on - strand, within Shew_0052 at 64.089 kb on - strand, within Shew_0052 at 64.094 kb on - strand, within Shew_0052 at 64.284 kb on + strand, within Shew_0052 at 64.292 kb on - strand, within Shew_0052 at 64.292 kb on - strand, within Shew_0052 at 64.326 kb on + strand, within Shew_0052 at 64.334 kb on + strand, within Shew_0052 at 64.611 kb on + strand at 64.619 kb on - strand at 64.673 kb on - strand at 64.701 kb on + strand at 64.836 kb on + strand, within Shew_0053 at 64.844 kb on - strand, within Shew_0053 at 64.919 kb on - strand, within Shew_0053 at 64.972 kb on - strand, within Shew_0053 at 64.990 kb on + strand, within Shew_0053 at 64.998 kb on - strand, within Shew_0053 at 65.037 kb on - strand, within Shew_0053 at 65.053 kb on - strand, within Shew_0053 at 65.053 kb on - strand, within Shew_0053 at 65.055 kb on + strand, within Shew_0053 at 65.055 kb on + strand, within Shew_0053 at 65.055 kb on + strand, within Shew_0053 at 65.055 kb on + strand, within Shew_0053 at 65.055 kb on + strand, within Shew_0053 at 65.062 kb on + strand, within Shew_0053 at 65.063 kb on - strand, within Shew_0053 at 65.063 kb on - strand, within Shew_0053 at 65.063 kb on - strand, within Shew_0053 at 65.070 kb on - strand, within Shew_0053 at 65.199 kb on - strand, within Shew_0053 at 65.394 kb on + strand, within Shew_0053 at 65.528 kb on + strand at 65.536 kb on - strand at 65.594 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 62,993 + Shew_0051 0.63 +0.5 63,001 - Shew_0051 0.63 +0.4 63,001 - Shew_0051 0.63 -3.5 63,001 - Shew_0051 0.63 -0.1 63,141 - Shew_0051 0.71 -0.4 63,293 + Shew_0051 0.79 -2.7 63,301 - Shew_0051 0.79 -0.1 63,301 - Shew_0051 0.79 +0.1 63,303 + Shew_0051 0.79 +0.6 63,333 + Shew_0051 0.81 +1.1 63,426 - Shew_0051 0.86 +2.1 63,474 + Shew_0051 0.88 -0.2 63,474 + Shew_0051 0.88 -0.6 63,526 + -0.6 63,806 - -0.7 63,834 + +0.4 63,836 + -2.1 64,081 + Shew_0052 0.32 -1.0 64,089 - Shew_0052 0.33 +0.1 64,089 - Shew_0052 0.33 -1.3 64,094 - Shew_0052 0.34 +0.2 64,284 + Shew_0052 0.55 +0.1 64,292 - Shew_0052 0.56 -0.8 64,292 - Shew_0052 0.56 -0.7 64,326 + Shew_0052 0.59 +0.3 64,334 + Shew_0052 0.60 -0.0 64,611 + +0.1 64,619 - +0.6 64,673 - -2.3 64,701 + -1.6 64,836 + Shew_0053 0.14 -2.1 64,844 - Shew_0053 0.15 +1.7 64,919 - Shew_0053 0.24 -1.9 64,972 - Shew_0053 0.30 -0.5 64,990 + Shew_0053 0.32 -2.8 64,998 - Shew_0053 0.33 -0.7 65,037 - Shew_0053 0.38 -0.3 65,053 - Shew_0053 0.40 -1.4 65,053 - Shew_0053 0.40 -0.4 65,055 + Shew_0053 0.40 -3.4 65,055 + Shew_0053 0.40 -1.1 65,055 + Shew_0053 0.40 -1.6 65,055 + Shew_0053 0.40 +0.2 65,055 + Shew_0053 0.40 -1.3 65,062 + Shew_0053 0.41 -2.5 65,063 - Shew_0053 0.41 -2.4 65,063 - Shew_0053 0.41 -1.5 65,063 - Shew_0053 0.41 -0.9 65,070 - Shew_0053 0.42 -0.8 65,199 - Shew_0053 0.57 -1.4 65,394 + Shew_0053 0.80 +0.2 65,528 + -1.4 65,536 - +1.6 65,594 + -3.4
Or see this region's nucleotide sequence