Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0032 and Shew_0033 are separated by 222 nucleotides Shew_0033 and Shew_0034 are separated by 61 nucleotides
Shew_0032: Shew_0032 - hydrophobe/amphiphile efflux-1 (HAE1) family protein (RefSeq), at 34,426 to 37,575
_0032
Shew_0033: Shew_0033 - peptidase M14, carboxypeptidase A (RefSeq), at 37,798 to 39,639
_0033
Shew_0034: Shew_0034 - methyltransferase type 12 (RefSeq), at 39,701 to 40,504
_0034
Position (kb)
37
38
39
40 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 37.182 kb on + strand, within Shew_0032 at 37.182 kb on + strand, within Shew_0032 at 37.207 kb on + strand, within Shew_0032 at 37.296 kb on + strand at 37.960 kb on + strand at 37.968 kb on - strand at 38.118 kb on - strand, within Shew_0033 at 38.295 kb on + strand, within Shew_0033 at 38.564 kb on + strand, within Shew_0033 at 38.869 kb on + strand, within Shew_0033 at 38.881 kb on + strand, within Shew_0033 at 38.998 kb on + strand, within Shew_0033 at 39.076 kb on + strand, within Shew_0033 at 39.084 kb on - strand, within Shew_0033 at 39.100 kb on - strand, within Shew_0033 at 39.145 kb on - strand, within Shew_0033 at 39.195 kb on + strand, within Shew_0033 at 39.195 kb on + strand, within Shew_0033 at 39.195 kb on + strand, within Shew_0033 at 39.203 kb on - strand, within Shew_0033 at 39.211 kb on - strand, within Shew_0033 at 39.211 kb on - strand, within Shew_0033 at 39.609 kb on + strand at 39.628 kb on - strand at 40.137 kb on + strand, within Shew_0034 at 40.145 kb on - strand, within Shew_0034 at 40.320 kb on + strand, within Shew_0034
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 37,182 + Shew_0032 0.87 -0.7 37,182 + Shew_0032 0.87 -1.5 37,207 + Shew_0032 0.88 +0.2 37,296 + -2.8 37,960 + -0.2 37,968 - -0.1 38,118 - Shew_0033 0.17 +0.7 38,295 + Shew_0033 0.27 -0.1 38,564 + Shew_0033 0.42 -1.5 38,869 + Shew_0033 0.58 +1.1 38,881 + Shew_0033 0.59 -1.1 38,998 + Shew_0033 0.65 -0.4 39,076 + Shew_0033 0.69 +0.7 39,084 - Shew_0033 0.70 -0.1 39,100 - Shew_0033 0.71 -1.1 39,145 - Shew_0033 0.73 +0.7 39,195 + Shew_0033 0.76 -1.3 39,195 + Shew_0033 0.76 +0.2 39,195 + Shew_0033 0.76 -0.4 39,203 - Shew_0033 0.76 -2.2 39,211 - Shew_0033 0.77 +1.4 39,211 - Shew_0033 0.77 -0.9 39,609 + -1.8 39,628 - -0.1 40,137 + Shew_0034 0.54 +0.7 40,145 - Shew_0034 0.55 +0.1 40,320 + Shew_0034 0.77 -0.1
Or see this region's nucleotide sequence