Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0014 and Shew_0015 are separated by 165 nucleotides Shew_0015 and Shew_0016 are separated by 133 nucleotides Shew_0016 and Shew_0017 overlap by 17 nucleotides Shew_0017 and fadA are separated by 354 nucleotides
Shew_0014: Shew_0014 - hypothetical protein (RefSeq), at 17,578 to 18,018
_0014
Shew_0015: Shew_0015 - SirA family protein (RefSeq), at 18,184 to 18,429
_0015
Shew_0016: Shew_0016 - ybaK/ebsC protein (RefSeq), at 18,563 to 19,027
_0016
Shew_0017: Shew_0017 - GCN5-related N-acetyltransferase (RefSeq), at 19,011 to 19,499
_0017
Shew_0018: fadA - 3-ketoacyl-CoA thiolase (RefSeq), at 19,854 to 21,017
fadA
Position (kb) 18
19
20 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 17.731 kb on - strand, within Shew_0014 at 17.744 kb on - strand, within Shew_0014 at 18.105 kb on + strand at 18.144 kb on - strand at 18.181 kb on + strand at 19.024 kb on + strand at 19.075 kb on + strand, within Shew_0017 at 19.170 kb on + strand, within Shew_0017 at 19.334 kb on + strand, within Shew_0017 at 19.339 kb on + strand, within Shew_0017 at 19.346 kb on + strand, within Shew_0017 at 19.347 kb on - strand, within Shew_0017 at 19.426 kb on + strand, within Shew_0017 at 19.771 kb on - strand at 19.871 kb on - strand at 19.900 kb on - strand at 19.960 kb on - strand at 19.961 kb on + strand at 19.965 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 17,731 - Shew_0014 0.35 +0.6 17,744 - Shew_0014 0.38 -3.2 18,105 + -0.5 18,144 - -1.1 18,181 + -0.9 19,024 + +0.3 19,075 + Shew_0017 0.13 +1.5 19,170 + Shew_0017 0.33 +0.6 19,334 + Shew_0017 0.66 -0.1 19,339 + Shew_0017 0.67 -1.0 19,346 + Shew_0017 0.69 +0.8 19,347 - Shew_0017 0.69 -1.3 19,426 + Shew_0017 0.85 +1.1 19,771 - -1.5 19,871 - -1.7 19,900 - +0.8 19,960 - -0.5 19,961 + +0.2 19,965 - +0.3
Or see this region's nucleotide sequence