Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0011 and Shew_0012 are separated by 5 nucleotides Shew_0012 and Shew_0013 are separated by 212 nucleotides Shew_0013 and Shew_0014 are separated by 302 nucleotides Shew_0014 and Shew_0015 are separated by 165 nucleotides
Shew_0011: Shew_0011 - DNA-3-methyladenine glycosylase I (RefSeq), at 12,604 to 13,173
_0011
Shew_0012: Shew_0012 - hypothetical protein (RefSeq), at 13,179 to 13,859
_0012
Shew_0013: Shew_0013 - amidohydrolase (RefSeq), at 14,072 to 17,275
_0013
Shew_0014: Shew_0014 - hypothetical protein (RefSeq), at 17,578 to 18,018
_0014
Shew_0015: Shew_0015 - SirA family protein (RefSeq), at 18,184 to 18,429
_0015
Position (kb) 14
15
16
17
18 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 13.201 kb on + strand at 13.245 kb on - strand at 13.247 kb on + strand, within Shew_0012 at 13.584 kb on - strand, within Shew_0012 at 13.675 kb on + strand, within Shew_0012 at 13.726 kb on + strand, within Shew_0012 at 13.726 kb on + strand, within Shew_0012 at 13.726 kb on + strand, within Shew_0012 at 13.726 kb on + strand, within Shew_0012 at 13.734 kb on - strand, within Shew_0012 at 13.734 kb on - strand, within Shew_0012 at 13.734 kb on - strand, within Shew_0012 at 13.786 kb on - strand, within Shew_0012 at 13.876 kb on + strand at 13.882 kb on - strand at 14.171 kb on - strand at 14.311 kb on + strand at 14.453 kb on + strand, within Shew_0013 at 14.490 kb on + strand, within Shew_0013 at 14.498 kb on - strand, within Shew_0013 at 14.530 kb on + strand, within Shew_0013 at 14.719 kb on + strand, within Shew_0013 at 14.726 kb on - strand, within Shew_0013 at 14.815 kb on + strand, within Shew_0013 at 14.830 kb on + strand, within Shew_0013 at 14.973 kb on + strand, within Shew_0013 at 14.973 kb on + strand, within Shew_0013 at 14.978 kb on + strand, within Shew_0013 at 14.981 kb on - strand, within Shew_0013 at 14.991 kb on - strand, within Shew_0013 at 15.002 kb on - strand, within Shew_0013 at 15.012 kb on - strand, within Shew_0013 at 15.065 kb on - strand, within Shew_0013 at 15.260 kb on - strand, within Shew_0013 at 15.262 kb on + strand, within Shew_0013 at 15.360 kb on + strand, within Shew_0013 at 15.426 kb on + strand, within Shew_0013 at 15.633 kb on + strand, within Shew_0013 at 15.680 kb on + strand, within Shew_0013 at 16.026 kb on + strand, within Shew_0013 at 16.275 kb on + strand, within Shew_0013 at 16.337 kb on + strand, within Shew_0013 at 16.444 kb on + strand, within Shew_0013 at 16.501 kb on + strand, within Shew_0013 at 16.616 kb on + strand, within Shew_0013 at 16.625 kb on - strand, within Shew_0013 at 16.641 kb on - strand, within Shew_0013 at 16.696 kb on + strand, within Shew_0013 at 16.765 kb on + strand, within Shew_0013 at 16.779 kb on - strand, within Shew_0013 at 17.000 kb on + strand at 17.118 kb on + strand at 17.126 kb on - strand at 17.236 kb on - strand at 17.731 kb on - strand, within Shew_0014 at 17.744 kb on - strand, within Shew_0014 at 18.105 kb on + strand at 18.144 kb on - strand at 18.181 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 13,201 + -0.3 13,245 - -0.6 13,247 + Shew_0012 0.10 +1.3 13,584 - Shew_0012 0.59 +2.7 13,675 + Shew_0012 0.73 -1.1 13,726 + Shew_0012 0.80 -1.3 13,726 + Shew_0012 0.80 -0.9 13,726 + Shew_0012 0.80 +0.2 13,726 + Shew_0012 0.80 +0.8 13,734 - Shew_0012 0.81 -0.9 13,734 - Shew_0012 0.81 +0.3 13,734 - Shew_0012 0.81 -0.3 13,786 - Shew_0012 0.89 +1.0 13,876 + +0.1 13,882 - +0.4 14,171 - +1.5 14,311 + +0.6 14,453 + Shew_0013 0.12 +2.8 14,490 + Shew_0013 0.13 -0.5 14,498 - Shew_0013 0.13 +1.1 14,530 + Shew_0013 0.14 +0.5 14,719 + Shew_0013 0.20 +1.1 14,726 - Shew_0013 0.20 -2.6 14,815 + Shew_0013 0.23 +0.3 14,830 + Shew_0013 0.24 +0.3 14,973 + Shew_0013 0.28 -0.7 14,973 + Shew_0013 0.28 +1.1 14,978 + Shew_0013 0.28 +0.7 14,981 - Shew_0013 0.28 +0.5 14,991 - Shew_0013 0.29 +0.2 15,002 - Shew_0013 0.29 -3.6 15,012 - Shew_0013 0.29 -1.3 15,065 - Shew_0013 0.31 -1.9 15,260 - Shew_0013 0.37 -0.3 15,262 + Shew_0013 0.37 -0.1 15,360 + Shew_0013 0.40 +1.5 15,426 + Shew_0013 0.42 +0.0 15,633 + Shew_0013 0.49 -0.9 15,680 + Shew_0013 0.50 +1.1 16,026 + Shew_0013 0.61 +0.2 16,275 + Shew_0013 0.69 +0.1 16,337 + Shew_0013 0.71 +0.6 16,444 + Shew_0013 0.74 -0.2 16,501 + Shew_0013 0.76 -0.6 16,616 + Shew_0013 0.79 -1.5 16,625 - Shew_0013 0.80 +0.9 16,641 - Shew_0013 0.80 +0.9 16,696 + Shew_0013 0.82 +0.1 16,765 + Shew_0013 0.84 -0.5 16,779 - Shew_0013 0.84 -1.1 17,000 + +0.8 17,118 + +0.7 17,126 - +0.4 17,236 - +0.3 17,731 - Shew_0014 0.35 +0.6 17,744 - Shew_0014 0.38 -3.2 18,105 + -0.5 18,144 - -1.1 18,181 + -0.9
Or see this region's nucleotide sequence