Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04400 and MMJJ_RS04405 are separated by 105 nucleotides MMJJ_RS04405 and MMJJ_RS04410 are separated by 83 nucleotides MMJJ_RS04410 and MMJJ_RS04415 are separated by 126 nucleotides MMJJ_RS04415 and MMJJ_RS04420 are separated by 569 nucleotides
MMJJ_RS04400: MMJJ_RS04400 - AAA family ATPase, at 821,438 to 823,261
_RS04400
MMJJ_RS04405: MMJJ_RS04405 - hypothetical protein, at 823,367 to 823,735
_RS04405
MMJJ_RS04410: MMJJ_RS04410 - hypothetical protein, at 823,819 to 824,229
_RS04410
MMJJ_RS04415: MMJJ_RS04415 - hypothetical protein, at 824,356 to 824,550
_RS04415
MMJJ_RS04420: MMJJ_RS04420 - type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family, at 825,120 to 825,407
_RS04420
Position (kb) 823
824
825 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 822.824 kb on + strand, within MMJJ_RS04400 at 822.824 kb on + strand, within MMJJ_RS04400 at 822.827 kb on + strand, within MMJJ_RS04400 at 822.827 kb on + strand, within MMJJ_RS04400 at 822.830 kb on + strand, within MMJJ_RS04400 at 822.830 kb on + strand, within MMJJ_RS04400 at 822.830 kb on + strand, within MMJJ_RS04400 at 822.830 kb on + strand, within MMJJ_RS04400 at 822.830 kb on + strand, within MMJJ_RS04400 at 822.830 kb on + strand, within MMJJ_RS04400 at 822.839 kb on + strand, within MMJJ_RS04400 at 822.844 kb on - strand, within MMJJ_RS04400 at 822.845 kb on + strand, within MMJJ_RS04400 at 822.846 kb on - strand, within MMJJ_RS04400 at 822.864 kb on - strand, within MMJJ_RS04400 at 822.864 kb on - strand, within MMJJ_RS04400 at 822.865 kb on + strand, within MMJJ_RS04400 at 822.868 kb on - strand, within MMJJ_RS04400 at 822.880 kb on - strand, within MMJJ_RS04400 at 822.881 kb on + strand, within MMJJ_RS04400 at 822.881 kb on + strand, within MMJJ_RS04400 at 822.882 kb on - strand, within MMJJ_RS04400 at 822.882 kb on - strand, within MMJJ_RS04400 at 822.888 kb on + strand, within MMJJ_RS04400 at 822.926 kb on - strand, within MMJJ_RS04400 at 822.926 kb on - strand, within MMJJ_RS04400 at 822.926 kb on - strand, within MMJJ_RS04400 at 822.926 kb on - strand, within MMJJ_RS04400 at 822.926 kb on - strand, within MMJJ_RS04400 at 822.927 kb on + strand, within MMJJ_RS04400 at 822.927 kb on + strand, within MMJJ_RS04400 at 822.950 kb on + strand, within MMJJ_RS04400 at 822.974 kb on + strand, within MMJJ_RS04400 at 822.976 kb on - strand, within MMJJ_RS04400 at 822.976 kb on - strand, within MMJJ_RS04400 at 822.976 kb on - strand, within MMJJ_RS04400 at 822.977 kb on + strand, within MMJJ_RS04400 at 822.982 kb on - strand, within MMJJ_RS04400 at 822.982 kb on - strand, within MMJJ_RS04400 at 822.982 kb on - strand, within MMJJ_RS04400 at 822.982 kb on - strand, within MMJJ_RS04400 at 822.997 kb on - strand, within MMJJ_RS04400 at 823.003 kb on - strand, within MMJJ_RS04400 at 823.030 kb on - strand, within MMJJ_RS04400 at 823.031 kb on + strand, within MMJJ_RS04400 at 823.031 kb on + strand, within MMJJ_RS04400 at 823.033 kb on + strand, within MMJJ_RS04400 at 823.033 kb on + strand, within MMJJ_RS04400 at 823.036 kb on - strand, within MMJJ_RS04400 at 823.036 kb on - strand, within MMJJ_RS04400 at 823.037 kb on + strand, within MMJJ_RS04400 at 823.046 kb on + strand, within MMJJ_RS04400 at 823.076 kb on + strand, within MMJJ_RS04400 at 823.085 kb on - strand at 823.086 kb on + strand at 823.086 kb on + strand at 823.089 kb on - strand at 823.089 kb on - strand at 823.089 kb on - strand at 823.136 kb on + strand at 823.139 kb on + strand at 823.157 kb on - strand at 823.174 kb on - strand at 823.174 kb on - strand at 823.175 kb on + strand at 823.175 kb on + strand at 823.175 kb on + strand at 823.176 kb on - strand at 823.201 kb on - strand at 823.205 kb on - strand at 823.206 kb on + strand at 823.217 kb on - strand at 823.217 kb on - strand at 823.234 kb on - strand at 823.234 kb on - strand at 823.237 kb on - strand at 823.237 kb on - strand at 823.237 kb on - strand at 823.237 kb on - strand at 823.238 kb on + strand at 823.238 kb on + strand at 823.238 kb on + strand at 823.241 kb on - strand at 823.241 kb on - strand at 823.242 kb on + strand at 823.256 kb on - strand at 823.257 kb on + strand at 823.291 kb on + strand at 823.292 kb on - strand at 823.292 kb on - strand at 823.296 kb on - strand at 823.296 kb on - strand at 823.296 kb on - strand at 823.297 kb on + strand at 823.301 kb on - strand at 823.302 kb on + strand at 823.306 kb on - strand at 823.307 kb on + strand at 823.317 kb on - strand at 823.317 kb on - strand at 823.317 kb on - strand at 823.318 kb on + strand at 823.318 kb on + strand at 823.318 kb on + strand at 823.368 kb on - strand at 823.393 kb on + strand at 823.417 kb on + strand, within MMJJ_RS04405 at 823.498 kb on - strand, within MMJJ_RS04405 at 823.498 kb on - strand, within MMJJ_RS04405 at 823.498 kb on - strand, within MMJJ_RS04405 at 823.504 kb on - strand, within MMJJ_RS04405 at 823.504 kb on - strand, within MMJJ_RS04405 at 823.504 kb on - strand, within MMJJ_RS04405 at 823.504 kb on - strand, within MMJJ_RS04405 at 823.504 kb on - strand, within MMJJ_RS04405 at 823.504 kb on - strand, within MMJJ_RS04405 at 823.505 kb on + strand, within MMJJ_RS04405 at 823.505 kb on + strand, within MMJJ_RS04405 at 823.505 kb on + strand, within MMJJ_RS04405 at 823.515 kb on + strand, within MMJJ_RS04405 at 823.515 kb on + strand, within MMJJ_RS04405 at 823.535 kb on - strand, within MMJJ_RS04405 at 823.535 kb on - strand, within MMJJ_RS04405 at 823.567 kb on - strand, within MMJJ_RS04405 at 823.572 kb on + strand, within MMJJ_RS04405 at 823.595 kb on - strand, within MMJJ_RS04405 at 823.595 kb on - strand, within MMJJ_RS04405 at 823.596 kb on + strand, within MMJJ_RS04405 at 823.608 kb on + strand, within MMJJ_RS04405 at 823.712 kb on - strand at 823.712 kb on - strand at 823.712 kb on - strand at 823.713 kb on + strand at 823.713 kb on + strand at 823.787 kb on + strand at 823.788 kb on - strand at 823.801 kb on - strand at 823.850 kb on + strand at 823.853 kb on - strand at 823.853 kb on - strand at 823.903 kb on - strand, within MMJJ_RS04410 at 824.026 kb on - strand, within MMJJ_RS04410 at 824.026 kb on - strand, within MMJJ_RS04410 at 824.069 kb on - strand, within MMJJ_RS04410 at 824.090 kb on - strand, within MMJJ_RS04410 at 824.118 kb on - strand, within MMJJ_RS04410 at 824.120 kb on - strand, within MMJJ_RS04410 at 824.121 kb on + strand, within MMJJ_RS04410 at 824.278 kb on - strand at 824.279 kb on + strand at 824.294 kb on - strand at 824.294 kb on - strand at 824.295 kb on + strand at 824.296 kb on - strand at 824.296 kb on - strand at 824.301 kb on - strand at 824.333 kb on - strand at 824.333 kb on - strand at 824.334 kb on + strand at 824.403 kb on + strand, within MMJJ_RS04415 at 824.445 kb on - strand, within MMJJ_RS04415 at 824.497 kb on + strand, within MMJJ_RS04415 at 824.497 kb on + strand, within MMJJ_RS04415 at 824.578 kb on - strand at 824.646 kb on - strand at 824.698 kb on + strand at 824.754 kb on - strand at 824.754 kb on - strand at 824.755 kb on + strand at 824.755 kb on + strand at 824.757 kb on - strand at 824.757 kb on - strand at 824.757 kb on - strand at 824.758 kb on + strand at 824.758 kb on + strand at 824.764 kb on - strand at 824.764 kb on - strand at 824.765 kb on + strand at 824.765 kb on + strand at 824.786 kb on + strand at 824.786 kb on + strand at 824.786 kb on + strand at 824.788 kb on - strand at 824.788 kb on - strand at 824.788 kb on - strand at 824.788 kb on - strand at 824.789 kb on + strand at 824.789 kb on + strand at 824.789 kb on + strand at 824.808 kb on - strand at 824.823 kb on + strand at 824.825 kb on - strand at 824.844 kb on + strand at 824.851 kb on - strand at 824.852 kb on + strand at 824.857 kb on - strand at 824.857 kb on - strand at 824.857 kb on - strand at 824.857 kb on - strand at 824.857 kb on - strand at 824.858 kb on + strand at 824.858 kb on + strand at 824.858 kb on + strand at 824.858 kb on + strand at 824.858 kb on + strand at 824.859 kb on - strand at 824.859 kb on - strand at 824.860 kb on + strand at 824.860 kb on + strand at 824.860 kb on + strand at 824.860 kb on + strand at 824.893 kb on + strand at 824.899 kb on + strand at 824.899 kb on + strand at 824.899 kb on + strand at 824.998 kb on - strand at 825.017 kb on + strand at 825.028 kb on - strand at 825.045 kb on - strand at 825.086 kb on - strand at 825.121 kb on - strand at 825.148 kb on + strand at 825.154 kb on - strand, within MMJJ_RS04420 at 825.154 kb on - strand, within MMJJ_RS04420 at 825.154 kb on - strand, within MMJJ_RS04420 at 825.155 kb on + strand, within MMJJ_RS04420 at 825.155 kb on + strand, within MMJJ_RS04420 at 825.155 kb on + strand, within MMJJ_RS04420 at 825.155 kb on + strand, within MMJJ_RS04420 at 825.156 kb on - strand, within MMJJ_RS04420 at 825.156 kb on - strand, within MMJJ_RS04420 at 825.156 kb on - strand, within MMJJ_RS04420 at 825.157 kb on + strand, within MMJJ_RS04420 at 825.157 kb on + strand, within MMJJ_RS04420 at 825.157 kb on + strand, within MMJJ_RS04420 at 825.182 kb on - strand, within MMJJ_RS04420 at 825.183 kb on + strand, within MMJJ_RS04420 at 825.183 kb on + strand, within MMJJ_RS04420 at 825.186 kb on - strand, within MMJJ_RS04420 at 825.187 kb on + strand, within MMJJ_RS04420 at 825.187 kb on + strand, within MMJJ_RS04420 at 825.187 kb on + strand, within MMJJ_RS04420 at 825.187 kb on + strand, within MMJJ_RS04420 at 825.189 kb on - strand, within MMJJ_RS04420 at 825.190 kb on + strand, within MMJJ_RS04420 at 825.192 kb on + strand, within MMJJ_RS04420 at 825.194 kb on - strand, within MMJJ_RS04420 at 825.194 kb on - strand, within MMJJ_RS04420 at 825.210 kb on + strand, within MMJJ_RS04420 at 825.212 kb on + strand, within MMJJ_RS04420
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 822,824 + MMJJ_RS04400 0.76 -0.8 822,824 + MMJJ_RS04400 0.76 +0.4 822,827 + MMJJ_RS04400 0.76 +1.1 822,827 + MMJJ_RS04400 0.76 +0.5 822,830 + MMJJ_RS04400 0.76 +0.9 822,830 + MMJJ_RS04400 0.76 -2.1 822,830 + MMJJ_RS04400 0.76 -0.1 822,830 + MMJJ_RS04400 0.76 +0.8 822,830 + MMJJ_RS04400 0.76 -0.6 822,830 + MMJJ_RS04400 0.76 -0.2 822,839 + MMJJ_RS04400 0.77 +0.5 822,844 - MMJJ_RS04400 0.77 +0.1 822,845 + MMJJ_RS04400 0.77 -0.4 822,846 - MMJJ_RS04400 0.77 +0.8 822,864 - MMJJ_RS04400 0.78 +1.5 822,864 - MMJJ_RS04400 0.78 -0.9 822,865 + MMJJ_RS04400 0.78 +0.1 822,868 - MMJJ_RS04400 0.78 -1.0 822,880 - MMJJ_RS04400 0.79 -1.0 822,881 + MMJJ_RS04400 0.79 +0.3 822,881 + MMJJ_RS04400 0.79 +0.2 822,882 - MMJJ_RS04400 0.79 +1.0 822,882 - MMJJ_RS04400 0.79 +0.5 822,888 + MMJJ_RS04400 0.79 -1.2 822,926 - MMJJ_RS04400 0.82 -0.3 822,926 - MMJJ_RS04400 0.82 +0.2 822,926 - MMJJ_RS04400 0.82 +0.2 822,926 - MMJJ_RS04400 0.82 +0.7 822,926 - MMJJ_RS04400 0.82 -0.2 822,927 + MMJJ_RS04400 0.82 +0.7 822,927 + MMJJ_RS04400 0.82 +0.8 822,950 + MMJJ_RS04400 0.83 -0.5 822,974 + MMJJ_RS04400 0.84 +0.1 822,976 - MMJJ_RS04400 0.84 -0.7 822,976 - MMJJ_RS04400 0.84 +0.5 822,976 - MMJJ_RS04400 0.84 +1.3 822,977 + MMJJ_RS04400 0.84 -1.0 822,982 - MMJJ_RS04400 0.85 -0.1 822,982 - MMJJ_RS04400 0.85 -0.2 822,982 - MMJJ_RS04400 0.85 -0.6 822,982 - MMJJ_RS04400 0.85 -1.7 822,997 - MMJJ_RS04400 0.85 -1.4 823,003 - MMJJ_RS04400 0.86 +0.1 823,030 - MMJJ_RS04400 0.87 +0.2 823,031 + MMJJ_RS04400 0.87 +0.5 823,031 + MMJJ_RS04400 0.87 -0.5 823,033 + MMJJ_RS04400 0.87 +0.7 823,033 + MMJJ_RS04400 0.87 +0.6 823,036 - MMJJ_RS04400 0.88 +0.5 823,036 - MMJJ_RS04400 0.88 +0.7 823,037 + MMJJ_RS04400 0.88 +0.6 823,046 + MMJJ_RS04400 0.88 -1.5 823,076 + MMJJ_RS04400 0.90 +0.6 823,085 - +1.0 823,086 + +0.4 823,086 + -0.6 823,089 - +0.6 823,089 - +0.9 823,089 - -0.3 823,136 + +0.5 823,139 + +0.2 823,157 - -0.0 823,174 - -0.5 823,174 - -0.6 823,175 + +0.1 823,175 + -0.6 823,175 + +0.8 823,176 - -2.1 823,201 - -2.0 823,205 - -0.6 823,206 + -0.8 823,217 - -1.0 823,217 - -1.3 823,234 - -0.5 823,234 - -1.1 823,237 - +0.6 823,237 - -0.1 823,237 - -0.6 823,237 - +0.2 823,238 + +0.0 823,238 + -0.9 823,238 + -0.3 823,241 - +0.9 823,241 - -2.0 823,242 + -1.2 823,256 - +0.3 823,257 + -1.0 823,291 + +0.3 823,292 - +0.6 823,292 - +0.5 823,296 - -0.5 823,296 - -0.6 823,296 - -0.1 823,297 + +0.7 823,301 - +0.6 823,302 + +0.4 823,306 - +0.5 823,307 + +0.4 823,317 - -0.4 823,317 - -1.0 823,317 - +0.8 823,318 + +0.2 823,318 + +0.3 823,318 + -0.6 823,368 - -0.0 823,393 + -1.5 823,417 + MMJJ_RS04405 0.14 -0.0 823,498 - MMJJ_RS04405 0.36 +0.1 823,498 - MMJJ_RS04405 0.36 +0.6 823,498 - MMJJ_RS04405 0.36 +0.5 823,504 - MMJJ_RS04405 0.37 +0.6 823,504 - MMJJ_RS04405 0.37 -0.9 823,504 - MMJJ_RS04405 0.37 -0.5 823,504 - MMJJ_RS04405 0.37 -0.7 823,504 - MMJJ_RS04405 0.37 +1.0 823,504 - MMJJ_RS04405 0.37 -0.1 823,505 + MMJJ_RS04405 0.37 +0.7 823,505 + MMJJ_RS04405 0.37 +0.8 823,505 + MMJJ_RS04405 0.37 -0.9 823,515 + MMJJ_RS04405 0.40 +1.0 823,515 + MMJJ_RS04405 0.40 -0.2 823,535 - MMJJ_RS04405 0.46 -0.0 823,535 - MMJJ_RS04405 0.46 -0.6 823,567 - MMJJ_RS04405 0.54 -0.2 823,572 + MMJJ_RS04405 0.56 -0.7 823,595 - MMJJ_RS04405 0.62 +0.6 823,595 - MMJJ_RS04405 0.62 -1.0 823,596 + MMJJ_RS04405 0.62 +0.9 823,608 + MMJJ_RS04405 0.65 -1.0 823,712 - +0.6 823,712 - -0.0 823,712 - -0.6 823,713 + +1.2 823,713 + -0.3 823,787 + +0.3 823,788 - +0.2 823,801 - +0.3 823,850 + +0.8 823,853 - +0.3 823,853 - +0.7 823,903 - MMJJ_RS04410 0.20 -0.8 824,026 - MMJJ_RS04410 0.50 +0.6 824,026 - MMJJ_RS04410 0.50 +0.7 824,069 - MMJJ_RS04410 0.61 -0.1 824,090 - MMJJ_RS04410 0.66 +0.7 824,118 - MMJJ_RS04410 0.73 +0.4 824,120 - MMJJ_RS04410 0.73 -0.5 824,121 + MMJJ_RS04410 0.73 +0.8 824,278 - -0.2 824,279 + -1.2 824,294 - -0.7 824,294 - +1.6 824,295 + +0.2 824,296 - +0.2 824,296 - +0.7 824,301 - -3.1 824,333 - +2.3 824,333 - +0.8 824,334 + +1.7 824,403 + MMJJ_RS04415 0.24 +1.5 824,445 - MMJJ_RS04415 0.46 +1.7 824,497 + MMJJ_RS04415 0.72 +1.5 824,497 + MMJJ_RS04415 0.72 +1.4 824,578 - -0.1 824,646 - +1.4 824,698 + +0.1 824,754 - -0.8 824,754 - +0.3 824,755 + +0.9 824,755 + +0.9 824,757 - -1.2 824,757 - +1.0 824,757 - -0.0 824,758 + +0.9 824,758 + -1.1 824,764 - -0.2 824,764 - +0.2 824,765 + -0.3 824,765 + -0.9 824,786 + -2.5 824,786 + -0.3 824,786 + +0.6 824,788 - +0.5 824,788 - -1.3 824,788 - +0.4 824,788 - +0.3 824,789 + +0.8 824,789 + +0.3 824,789 + -0.1 824,808 - +0.7 824,823 + +0.4 824,825 - +1.1 824,844 + +0.4 824,851 - -0.1 824,852 + +0.5 824,857 - -0.8 824,857 - +0.4 824,857 - +0.6 824,857 - -0.4 824,857 - +0.6 824,858 + -1.4 824,858 + +0.3 824,858 + +0.8 824,858 + +0.1 824,858 + -0.4 824,859 - +0.1 824,859 - +0.5 824,860 + +0.1 824,860 + +1.5 824,860 + -1.2 824,860 + +0.7 824,893 + +0.4 824,899 + -0.8 824,899 + -0.1 824,899 + -0.0 824,998 - -0.7 825,017 + +0.6 825,028 - -0.2 825,045 - +0.9 825,086 - -0.6 825,121 - +1.1 825,148 + -0.6 825,154 - MMJJ_RS04420 0.12 +0.8 825,154 - MMJJ_RS04420 0.12 +0.4 825,154 - MMJJ_RS04420 0.12 +0.3 825,155 + MMJJ_RS04420 0.12 +0.4 825,155 + MMJJ_RS04420 0.12 +0.6 825,155 + MMJJ_RS04420 0.12 +0.4 825,155 + MMJJ_RS04420 0.12 -0.1 825,156 - MMJJ_RS04420 0.12 -0.6 825,156 - MMJJ_RS04420 0.12 +0.8 825,156 - MMJJ_RS04420 0.12 -0.5 825,157 + MMJJ_RS04420 0.13 +0.3 825,157 + MMJJ_RS04420 0.13 +0.6 825,157 + MMJJ_RS04420 0.13 +0.6 825,182 - MMJJ_RS04420 0.22 +0.4 825,183 + MMJJ_RS04420 0.22 -1.8 825,183 + MMJJ_RS04420 0.22 -0.8 825,186 - MMJJ_RS04420 0.23 -1.4 825,187 + MMJJ_RS04420 0.23 +0.8 825,187 + MMJJ_RS04420 0.23 +0.7 825,187 + MMJJ_RS04420 0.23 +0.8 825,187 + MMJJ_RS04420 0.23 -0.1 825,189 - MMJJ_RS04420 0.24 +1.0 825,190 + MMJJ_RS04420 0.24 -0.7 825,192 + MMJJ_RS04420 0.25 +0.3 825,194 - MMJJ_RS04420 0.26 +1.3 825,194 - MMJJ_RS04420 0.26 -0.9 825,210 + MMJJ_RS04420 0.31 -0.1 825,212 + MMJJ_RS04420 0.32 +1.1
Or see this region's nucleotide sequence