Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00800 and MMJJ_RS00805 are separated by 67 nucleotides MMJJ_RS00805 and MMJJ_RS00810 are separated by 5 nucleotides MMJJ_RS00810 and MMJJ_RS00815 are separated by 51 nucleotides
MMJJ_RS00800: MMJJ_RS00800 - MFS transporter, at 151,424 to 152,728
_RS00800
MMJJ_RS00805: MMJJ_RS00805 - uroporphyrinogen decarboxylase, at 152,796 to 153,659
_RS00805
MMJJ_RS00810: MMJJ_RS00810 - GTP-binding protein, at 153,665 to 154,573
_RS00810
MMJJ_RS00815: MMJJ_RS00815 - ASKHA domain-containing protein, at 154,625 to 156,403
_RS00815
Position (kb) 153
154
155 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 152.665 kb on + strand at 152.727 kb on + strand at 152.769 kb on + strand at 152.779 kb on - strand at 152.780 kb on + strand at 152.780 kb on + strand at 152.796 kb on + strand at 152.885 kb on + strand, within MMJJ_RS00805 at 152.936 kb on - strand, within MMJJ_RS00805 at 152.937 kb on + strand, within MMJJ_RS00805 at 152.937 kb on + strand, within MMJJ_RS00805 at 152.937 kb on + strand, within MMJJ_RS00805 at 152.947 kb on - strand, within MMJJ_RS00805 at 152.948 kb on + strand, within MMJJ_RS00805 at 152.970 kb on + strand, within MMJJ_RS00805 at 152.970 kb on + strand, within MMJJ_RS00805 at 153.071 kb on + strand, within MMJJ_RS00805 at 153.185 kb on - strand, within MMJJ_RS00805 at 153.185 kb on - strand, within MMJJ_RS00805 at 153.185 kb on - strand, within MMJJ_RS00805 at 153.186 kb on + strand, within MMJJ_RS00805 at 153.186 kb on + strand, within MMJJ_RS00805 at 153.189 kb on + strand, within MMJJ_RS00805 at 153.189 kb on + strand, within MMJJ_RS00805 at 153.223 kb on + strand, within MMJJ_RS00805 at 153.289 kb on - strand, within MMJJ_RS00805 at 153.294 kb on - strand, within MMJJ_RS00805 at 153.295 kb on + strand, within MMJJ_RS00805 at 153.295 kb on + strand, within MMJJ_RS00805 at 153.295 kb on + strand, within MMJJ_RS00805 at 153.295 kb on + strand, within MMJJ_RS00805 at 153.364 kb on - strand, within MMJJ_RS00805 at 153.390 kb on - strand, within MMJJ_RS00805 at 153.390 kb on - strand, within MMJJ_RS00805 at 153.434 kb on + strand, within MMJJ_RS00805 at 153.449 kb on - strand, within MMJJ_RS00805 at 153.452 kb on - strand, within MMJJ_RS00805 at 153.452 kb on - strand, within MMJJ_RS00805 at 153.453 kb on + strand, within MMJJ_RS00805 at 153.537 kb on - strand, within MMJJ_RS00805 at 153.537 kb on - strand, within MMJJ_RS00805 at 153.538 kb on + strand, within MMJJ_RS00805 at 153.538 kb on + strand, within MMJJ_RS00805 at 153.559 kb on - strand, within MMJJ_RS00805 at 153.560 kb on + strand, within MMJJ_RS00805 at 153.602 kb on - strand at 153.602 kb on - strand at 153.603 kb on + strand at 153.603 kb on + strand at 153.603 kb on + strand at 153.604 kb on - strand at 153.618 kb on - strand at 153.620 kb on - strand at 153.621 kb on + strand at 153.621 kb on + strand at 153.658 kb on + strand at 153.661 kb on - strand at 153.661 kb on - strand at 153.662 kb on + strand at 153.673 kb on - strand at 153.673 kb on - strand at 153.673 kb on - strand at 153.677 kb on + strand at 153.680 kb on - strand at 153.690 kb on + strand at 153.720 kb on + strand at 153.780 kb on - strand, within MMJJ_RS00810 at 153.780 kb on - strand, within MMJJ_RS00810 at 153.781 kb on + strand, within MMJJ_RS00810 at 153.781 kb on + strand, within MMJJ_RS00810 at 153.812 kb on - strand, within MMJJ_RS00810 at 153.833 kb on - strand, within MMJJ_RS00810 at 153.834 kb on + strand, within MMJJ_RS00810 at 153.843 kb on - strand, within MMJJ_RS00810 at 153.856 kb on - strand, within MMJJ_RS00810 at 153.962 kb on - strand, within MMJJ_RS00810 at 153.962 kb on - strand, within MMJJ_RS00810 at 153.963 kb on + strand, within MMJJ_RS00810 at 154.021 kb on - strand, within MMJJ_RS00810 at 154.041 kb on + strand, within MMJJ_RS00810 at 154.041 kb on + strand, within MMJJ_RS00810 at 154.047 kb on + strand, within MMJJ_RS00810 at 154.054 kb on - strand, within MMJJ_RS00810 at 154.055 kb on + strand, within MMJJ_RS00810 at 154.061 kb on - strand, within MMJJ_RS00810 at 154.062 kb on + strand, within MMJJ_RS00810 at 154.076 kb on - strand, within MMJJ_RS00810 at 154.082 kb on + strand, within MMJJ_RS00810 at 154.109 kb on + strand, within MMJJ_RS00810 at 154.121 kb on + strand, within MMJJ_RS00810 at 154.127 kb on + strand, within MMJJ_RS00810 at 154.130 kb on - strand, within MMJJ_RS00810 at 154.131 kb on + strand, within MMJJ_RS00810 at 154.131 kb on + strand, within MMJJ_RS00810 at 154.211 kb on - strand, within MMJJ_RS00810 at 154.229 kb on - strand, within MMJJ_RS00810 at 154.230 kb on + strand, within MMJJ_RS00810 at 154.284 kb on + strand, within MMJJ_RS00810 at 154.284 kb on + strand, within MMJJ_RS00810 at 154.337 kb on - strand, within MMJJ_RS00810 at 154.407 kb on - strand, within MMJJ_RS00810 at 154.409 kb on - strand, within MMJJ_RS00810 at 154.409 kb on - strand, within MMJJ_RS00810 at 154.410 kb on + strand, within MMJJ_RS00810 at 154.410 kb on + strand, within MMJJ_RS00810 at 154.432 kb on - strand, within MMJJ_RS00810 at 154.481 kb on - strand, within MMJJ_RS00810 at 154.482 kb on + strand, within MMJJ_RS00810 at 154.506 kb on + strand at 154.517 kb on - strand at 154.518 kb on + strand at 154.520 kb on + strand at 154.553 kb on - strand at 154.554 kb on + strand at 154.576 kb on - strand at 154.586 kb on - strand at 154.607 kb on - strand at 154.683 kb on - strand at 154.684 kb on + strand at 154.686 kb on - strand at 154.687 kb on + strand at 154.687 kb on + strand at 154.687 kb on + strand at 154.687 kb on + strand at 154.691 kb on - strand at 154.691 kb on - strand at 154.693 kb on - strand at 154.693 kb on - strand at 154.694 kb on + strand at 154.694 kb on + strand at 154.694 kb on + strand at 154.778 kb on + strand at 154.778 kb on + strand at 154.779 kb on - strand at 154.779 kb on - strand at 154.779 kb on - strand at 154.779 kb on - strand at 154.779 kb on - strand at 154.780 kb on + strand at 154.780 kb on + strand at 154.780 kb on + strand at 154.780 kb on + strand at 154.809 kb on + strand, within MMJJ_RS00815 at 154.869 kb on + strand, within MMJJ_RS00815 at 154.869 kb on + strand, within MMJJ_RS00815 at 154.871 kb on - strand, within MMJJ_RS00815 at 154.934 kb on - strand, within MMJJ_RS00815 at 154.935 kb on + strand, within MMJJ_RS00815 at 154.935 kb on + strand, within MMJJ_RS00815 at 154.964 kb on - strand, within MMJJ_RS00815 at 154.964 kb on - strand, within MMJJ_RS00815 at 154.964 kb on - strand, within MMJJ_RS00815 at 154.965 kb on + strand, within MMJJ_RS00815 at 154.965 kb on + strand, within MMJJ_RS00815 at 154.982 kb on - strand, within MMJJ_RS00815 at 155.033 kb on - strand, within MMJJ_RS00815 at 155.036 kb on - strand, within MMJJ_RS00815 at 155.048 kb on + strand, within MMJJ_RS00815 at 155.056 kb on - strand, within MMJJ_RS00815 at 155.184 kb on + strand, within MMJJ_RS00815 at 155.252 kb on - strand, within MMJJ_RS00815 at 155.252 kb on - strand, within MMJJ_RS00815 at 155.306 kb on + strand, within MMJJ_RS00815 at 155.306 kb on + strand, within MMJJ_RS00815 at 155.325 kb on + strand, within MMJJ_RS00815 at 155.339 kb on + strand, within MMJJ_RS00815 at 155.390 kb on - strand, within MMJJ_RS00815 at 155.390 kb on - strand, within MMJJ_RS00815 at 155.419 kb on - strand, within MMJJ_RS00815 at 155.419 kb on - strand, within MMJJ_RS00815 at 155.422 kb on + strand, within MMJJ_RS00815 at 155.467 kb on - strand, within MMJJ_RS00815 at 155.519 kb on - strand, within MMJJ_RS00815 at 155.520 kb on + strand, within MMJJ_RS00815 at 155.523 kb on - strand, within MMJJ_RS00815 at 155.524 kb on + strand, within MMJJ_RS00815
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 152,665 + -0.8 152,727 + +1.2 152,769 + +0.2 152,779 - -0.6 152,780 + +0.9 152,780 + +0.6 152,796 + +1.3 152,885 + MMJJ_RS00805 0.10 +0.3 152,936 - MMJJ_RS00805 0.16 +0.4 152,937 + MMJJ_RS00805 0.16 -0.5 152,937 + MMJJ_RS00805 0.16 -0.7 152,937 + MMJJ_RS00805 0.16 -2.3 152,947 - MMJJ_RS00805 0.17 +0.6 152,948 + MMJJ_RS00805 0.18 -1.0 152,970 + MMJJ_RS00805 0.20 +0.7 152,970 + MMJJ_RS00805 0.20 -0.2 153,071 + MMJJ_RS00805 0.32 -0.5 153,185 - MMJJ_RS00805 0.45 +0.8 153,185 - MMJJ_RS00805 0.45 +0.6 153,185 - MMJJ_RS00805 0.45 -1.0 153,186 + MMJJ_RS00805 0.45 +0.4 153,186 + MMJJ_RS00805 0.45 -0.4 153,189 + MMJJ_RS00805 0.45 +0.4 153,189 + MMJJ_RS00805 0.45 -2.5 153,223 + MMJJ_RS00805 0.49 +0.3 153,289 - MMJJ_RS00805 0.57 +0.4 153,294 - MMJJ_RS00805 0.58 -0.3 153,295 + MMJJ_RS00805 0.58 +1.0 153,295 + MMJJ_RS00805 0.58 -2.2 153,295 + MMJJ_RS00805 0.58 -0.1 153,295 + MMJJ_RS00805 0.58 +0.8 153,364 - MMJJ_RS00805 0.66 +0.6 153,390 - MMJJ_RS00805 0.69 -0.3 153,390 - MMJJ_RS00805 0.69 -0.4 153,434 + MMJJ_RS00805 0.74 +0.5 153,449 - MMJJ_RS00805 0.76 +0.3 153,452 - MMJJ_RS00805 0.76 -1.5 153,452 - MMJJ_RS00805 0.76 +0.2 153,453 + MMJJ_RS00805 0.76 -1.7 153,537 - MMJJ_RS00805 0.86 +0.5 153,537 - MMJJ_RS00805 0.86 +0.6 153,538 + MMJJ_RS00805 0.86 -0.7 153,538 + MMJJ_RS00805 0.86 +0.5 153,559 - MMJJ_RS00805 0.88 +0.8 153,560 + MMJJ_RS00805 0.88 -0.9 153,602 - +1.0 153,602 - +0.2 153,603 + +0.0 153,603 + +0.0 153,603 + +0.4 153,604 - +1.2 153,618 - -0.1 153,620 - -0.8 153,621 + +0.3 153,621 + -0.1 153,658 + +0.3 153,661 - -0.6 153,661 - +0.1 153,662 + +1.1 153,673 - +0.5 153,673 - -1.4 153,673 - -0.6 153,677 + +0.4 153,680 - +0.0 153,690 + +0.2 153,720 + +1.0 153,780 - MMJJ_RS00810 0.13 -0.3 153,780 - MMJJ_RS00810 0.13 +1.3 153,781 + MMJJ_RS00810 0.13 -0.4 153,781 + MMJJ_RS00810 0.13 -0.5 153,812 - MMJJ_RS00810 0.16 +1.2 153,833 - MMJJ_RS00810 0.18 +0.3 153,834 + MMJJ_RS00810 0.19 -1.3 153,843 - MMJJ_RS00810 0.20 +1.1 153,856 - MMJJ_RS00810 0.21 +0.3 153,962 - MMJJ_RS00810 0.33 -0.7 153,962 - MMJJ_RS00810 0.33 +0.7 153,963 + MMJJ_RS00810 0.33 +0.7 154,021 - MMJJ_RS00810 0.39 -1.0 154,041 + MMJJ_RS00810 0.41 +0.9 154,041 + MMJJ_RS00810 0.41 -0.3 154,047 + MMJJ_RS00810 0.42 +0.4 154,054 - MMJJ_RS00810 0.43 -1.6 154,055 + MMJJ_RS00810 0.43 -0.4 154,061 - MMJJ_RS00810 0.44 -0.4 154,062 + MMJJ_RS00810 0.44 +0.8 154,076 - MMJJ_RS00810 0.45 +0.5 154,082 + MMJJ_RS00810 0.46 -0.1 154,109 + MMJJ_RS00810 0.49 +0.1 154,121 + MMJJ_RS00810 0.50 -0.6 154,127 + MMJJ_RS00810 0.51 +1.0 154,130 - MMJJ_RS00810 0.51 +0.4 154,131 + MMJJ_RS00810 0.51 +1.4 154,131 + MMJJ_RS00810 0.51 +1.4 154,211 - MMJJ_RS00810 0.60 +0.7 154,229 - MMJJ_RS00810 0.62 +0.7 154,230 + MMJJ_RS00810 0.62 -1.0 154,284 + MMJJ_RS00810 0.68 +1.4 154,284 + MMJJ_RS00810 0.68 -1.4 154,337 - MMJJ_RS00810 0.74 +0.6 154,407 - MMJJ_RS00810 0.82 +0.1 154,409 - MMJJ_RS00810 0.82 -0.9 154,409 - MMJJ_RS00810 0.82 -1.0 154,410 + MMJJ_RS00810 0.82 +0.7 154,410 + MMJJ_RS00810 0.82 -0.6 154,432 - MMJJ_RS00810 0.84 +0.7 154,481 - MMJJ_RS00810 0.90 -0.1 154,482 + MMJJ_RS00810 0.90 +0.7 154,506 + +0.8 154,517 - -0.5 154,518 + -0.4 154,520 + -0.4 154,553 - +0.0 154,554 + +0.6 154,576 - +0.7 154,586 - -0.9 154,607 - +0.3 154,683 - +1.3 154,684 + -0.6 154,686 - +0.5 154,687 + -0.3 154,687 + +2.0 154,687 + -0.2 154,687 + +1.5 154,691 - -0.1 154,691 - +0.0 154,693 - -3.0 154,693 - -1.1 154,694 + +0.2 154,694 + -1.3 154,694 + -0.6 154,778 + -1.4 154,778 + +0.1 154,779 - -0.7 154,779 - -0.3 154,779 - +0.3 154,779 - -0.8 154,779 - -0.5 154,780 + +0.4 154,780 + -0.8 154,780 + +0.7 154,780 + +0.5 154,809 + MMJJ_RS00815 0.10 +0.1 154,869 + MMJJ_RS00815 0.14 -0.4 154,869 + MMJJ_RS00815 0.14 -1.3 154,871 - MMJJ_RS00815 0.14 +0.3 154,934 - MMJJ_RS00815 0.17 -2.3 154,935 + MMJJ_RS00815 0.17 -0.6 154,935 + MMJJ_RS00815 0.17 +1.1 154,964 - MMJJ_RS00815 0.19 +1.3 154,964 - MMJJ_RS00815 0.19 +0.8 154,964 - MMJJ_RS00815 0.19 +0.5 154,965 + MMJJ_RS00815 0.19 +0.9 154,965 + MMJJ_RS00815 0.19 +0.2 154,982 - MMJJ_RS00815 0.20 -1.3 155,033 - MMJJ_RS00815 0.23 +0.7 155,036 - MMJJ_RS00815 0.23 -0.8 155,048 + MMJJ_RS00815 0.24 +0.2 155,056 - MMJJ_RS00815 0.24 -1.0 155,184 + MMJJ_RS00815 0.31 -1.3 155,252 - MMJJ_RS00815 0.35 +0.6 155,252 - MMJJ_RS00815 0.35 +0.5 155,306 + MMJJ_RS00815 0.38 -3.2 155,306 + MMJJ_RS00815 0.38 +0.2 155,325 + MMJJ_RS00815 0.39 -1.4 155,339 + MMJJ_RS00815 0.40 +0.3 155,390 - MMJJ_RS00815 0.43 -0.5 155,390 - MMJJ_RS00815 0.43 +1.4 155,419 - MMJJ_RS00815 0.45 +0.7 155,419 - MMJJ_RS00815 0.45 +0.7 155,422 + MMJJ_RS00815 0.45 -0.8 155,467 - MMJJ_RS00815 0.47 +0.6 155,519 - MMJJ_RS00815 0.50 -1.7 155,520 + MMJJ_RS00815 0.50 -1.3 155,523 - MMJJ_RS00815 0.50 +1.0 155,524 + MMJJ_RS00815 0.51 -2.8
Or see this region's nucleotide sequence