Experiment: MoYLS4 with Polymyxin B sulfate 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0601 and DvMF_0602 are separated by 517 nucleotides DvMF_0602 and DvMF_0603 are separated by 4 nucleotides DvMF_0603 and DvMF_0604 are separated by 7 nucleotides DvMF_0604 and DvMF_0605 overlap by 4 nucleotides
DvMF_0601: DvMF_0601 - hypothetical protein (RefSeq), at 714,475 to 714,708
_0601
DvMF_0602: DvMF_0602 - 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq), at 715,226 to 715,552
_0602
DvMF_0603: DvMF_0603 - 2-oxoglutarate ferredoxin oxidoreductase subunit alpha (RefSeq), at 715,557 to 716,705
_0603
DvMF_0604: DvMF_0604 - 2-oxoglutarate ferredoxin oxidoreductase subunit beta (RefSeq), at 716,713 to 717,546
_0604
DvMF_0605: DvMF_0605 - pyruvate ferredoxin/flavodoxin oxidoreductase (RefSeq), at 717,543 to 718,166
_0605
Position (kb)
715
716
717 Strain fitness (log2 ratio)
-1
0
1 at 714.594 kb on + strand, within DvMF_0601 at 714.602 kb on - strand, within DvMF_0601 at 714.888 kb on + strand at 715.034 kb on + strand at 715.309 kb on - strand, within DvMF_0602 at 715.401 kb on + strand, within DvMF_0602 at 715.404 kb on - strand, within DvMF_0602 at 715.541 kb on + strand at 715.985 kb on + strand, within DvMF_0603 at 716.127 kb on - strand, within DvMF_0603 at 716.697 kb on + strand at 716.918 kb on - strand, within DvMF_0604 at 717.096 kb on + strand, within DvMF_0604 at 717.096 kb on + strand, within DvMF_0604 at 717.128 kb on - strand, within DvMF_0604 at 717.204 kb on + strand, within DvMF_0604 at 717.204 kb on + strand, within DvMF_0604 at 717.332 kb on - strand, within DvMF_0604 at 717.401 kb on - strand, within DvMF_0604
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.0625 mg/ml remove 714,594 + DvMF_0601 0.51 -0.2 714,602 - DvMF_0601 0.54 -0.3 714,888 + -0.3 715,034 + +0.2 715,309 - DvMF_0602 0.25 -0.7 715,401 + DvMF_0602 0.54 -0.1 715,404 - DvMF_0602 0.54 -1.0 715,541 + -0.8 715,985 + DvMF_0603 0.37 -1.2 716,127 - DvMF_0603 0.50 -0.5 716,697 + -0.1 716,918 - DvMF_0604 0.25 -0.3 717,096 + DvMF_0604 0.46 -0.3 717,096 + DvMF_0604 0.46 -0.1 717,128 - DvMF_0604 0.50 -1.0 717,204 + DvMF_0604 0.59 -0.6 717,204 + DvMF_0604 0.59 -0.3 717,332 - DvMF_0604 0.74 -0.9 717,401 - DvMF_0604 0.82 -0.4
Or see this region's nucleotide sequence