Experiment: MoYLS4 with Polymyxin B sulfate 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt secD and DvMF_0542 are separated by 125 nucleotides DvMF_0542 and DvMF_0543 are separated by 224 nucleotides DvMF_0543 and DvMF_0544 are separated by 317 nucleotides
DvMF_0541: secD - preprotein translocase subunit SecD (RefSeq), at 636,283 to 637,881
secD
DvMF_0542: DvMF_0542 - preprotein translocase, YajC subunit (RefSeq), at 638,007 to 638,408
_0542
DvMF_0543: DvMF_0543 - sodium/hydrogen exchanger (RefSeq), at 638,633 to 640,528
_0543
DvMF_0544: DvMF_0544 - glutamate synthase alpha subunit domain protein (RefSeq), at 640,846 to 641,691
_0544
Position (kb) 638
639
640
641 Strain fitness (log2 ratio) -2
-1
0
1 at 638.167 kb on + strand, within DvMF_0542 at 638.220 kb on - strand, within DvMF_0542 at 638.522 kb on + strand at 638.553 kb on - strand at 638.675 kb on - strand at 638.755 kb on + strand at 639.086 kb on + strand, within DvMF_0543 at 639.126 kb on + strand, within DvMF_0543 at 639.131 kb on + strand, within DvMF_0543 at 639.182 kb on - strand, within DvMF_0543 at 639.512 kb on + strand, within DvMF_0543 at 639.531 kb on + strand, within DvMF_0543 at 639.531 kb on + strand, within DvMF_0543 at 639.602 kb on + strand, within DvMF_0543 at 639.734 kb on + strand, within DvMF_0543 at 639.885 kb on + strand, within DvMF_0543 at 639.939 kb on - strand, within DvMF_0543 at 639.954 kb on - strand, within DvMF_0543 at 640.185 kb on + strand, within DvMF_0543 at 640.196 kb on + strand, within DvMF_0543 at 640.469 kb on + strand at 640.534 kb on + strand at 640.544 kb on + strand at 640.544 kb on + strand at 640.621 kb on + strand at 640.801 kb on + strand at 640.809 kb on - strand at 640.886 kb on + strand at 640.900 kb on - strand at 641.064 kb on + strand, within DvMF_0544 at 641.106 kb on - strand, within DvMF_0544 at 641.341 kb on - strand, within DvMF_0544 at 641.346 kb on - strand, within DvMF_0544
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.0625 mg/ml remove 638,167 + DvMF_0542 0.40 -2.3 638,220 - DvMF_0542 0.53 -2.4 638,522 + -0.3 638,553 - +0.2 638,675 - -0.4 638,755 + -0.3 639,086 + DvMF_0543 0.24 -0.3 639,126 + DvMF_0543 0.26 -0.4 639,131 + DvMF_0543 0.26 -0.4 639,182 - DvMF_0543 0.29 -1.0 639,512 + DvMF_0543 0.46 -0.6 639,531 + DvMF_0543 0.47 +0.6 639,531 + DvMF_0543 0.47 -2.0 639,602 + DvMF_0543 0.51 +0.2 639,734 + DvMF_0543 0.58 -0.8 639,885 + DvMF_0543 0.66 -0.3 639,939 - DvMF_0543 0.69 -0.2 639,954 - DvMF_0543 0.70 -0.2 640,185 + DvMF_0543 0.82 -0.3 640,196 + DvMF_0543 0.82 -0.3 640,469 + -0.4 640,534 + -0.3 640,544 + -0.1 640,544 + -0.5 640,621 + -0.2 640,801 + -0.2 640,809 - -0.2 640,886 + +0.1 640,900 - -0.3 641,064 + DvMF_0544 0.26 -0.2 641,106 - DvMF_0544 0.31 -0.7 641,341 - DvMF_0544 0.59 +0.2 641,346 - DvMF_0544 0.59 -0.0
Or see this region's nucleotide sequence