Strain Fitness in Shewanella loihica PV-4 around Shew_1080

Experiment: L-Malic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthrA and Shew_1080 overlap by 4 nucleotidesShew_1080 and Shew_1081 are separated by 13 nucleotides Shew_1079: thrA - bifunctional aspartokinase I/homeserine dehydrogenase I (RefSeq), at 1,241,698 to 1,244,166 thrA Shew_1080: Shew_1080 - homoserine kinase (RefSeq), at 1,244,163 to 1,245,113 _1080 Shew_1081: Shew_1081 - threonine synthase (RefSeq), at 1,245,127 to 1,246,410 _1081 Position (kb) 1244 1245 1246Strain fitness (log2 ratio) -8 -7 -6 -5 -4 -3 -2 -1 0 1at 1243.338 kb on + strand, within thrAat 1243.338 kb on + strand, within thrAat 1243.346 kb on - strand, within thrAat 1243.727 kb on + strand, within thrAat 1243.735 kb on - strand, within thrAat 1243.735 kb on - strand, within thrAat 1244.135 kb on + strandat 1244.159 kb on - strandat 1244.415 kb on + strand, within Shew_1080at 1244.689 kb on - strand, within Shew_1080at 1245.327 kb on + strand, within Shew_1081at 1245.386 kb on - strand, within Shew_1081at 1245.518 kb on + strand, within Shew_1081at 1245.564 kb on + strand, within Shew_1081at 1246.027 kb on - strand, within Shew_1081

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C)
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1,243,338 + thrA Shew_1079 0.66 -5.0
1,243,338 + thrA Shew_1079 0.66 -4.4
1,243,346 - thrA Shew_1079 0.67 -4.8
1,243,727 + thrA Shew_1079 0.82 -5.9
1,243,735 - thrA Shew_1079 0.83 -5.0
1,243,735 - thrA Shew_1079 0.83 -3.3
1,244,135 + -6.2
1,244,159 - -5.2
1,244,415 + Shew_1080 0.26 -4.3
1,244,689 - Shew_1080 0.55 -3.3
1,245,327 + Shew_1081 0.16 -8.7
1,245,386 - Shew_1081 0.20 -2.6
1,245,518 + Shew_1081 0.30 -6.8
1,245,564 + Shew_1081 0.34 -4.5
1,246,027 - Shew_1081 0.70 -6.8

Or see this region's nucleotide sequence