Experiment: Flask growth in 60 uE Light-Dark 8:16
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrS and xthA are separated by 86 nucleotides xthA and Synpcc7942_2608 are separated by 100 nucleotides Synpcc7942_2608 and Synpcc7942_2609 are separated by 201 nucleotides Synpcc7942_2609 and cobA overlap by 4 nucleotides
Synpcc7942_2606: thrS - threonyl-tRNA synthetase, at 2,688,305 to 2,690,119
thrS
Synpcc7942_2607: xthA - Exodeoxyribonuclease III, at 2,690,206 to 2,691,003
xthA
Synpcc7942_2608: Synpcc7942_2608 - hypothetical protein, at 2,691,104 to 2,691,424
_2608
Synpcc7942_2609: Synpcc7942_2609 - hypothetical protein, at 2,691,626 to 2,692,156
_2609
Synpcc7942_2610: cobA - uroporphyrin-III C-methyltransferase, at 2,692,153 to 2,692,950
cobA
Position (kb)
2691
2692 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2690.192 kb on + strand at 2690.192 kb on + strand at 2690.200 kb on - strand at 2690.200 kb on - strand at 2690.229 kb on + strand at 2690.236 kb on + strand at 2690.237 kb on - strand at 2690.253 kb on + strand at 2690.253 kb on + strand at 2690.261 kb on - strand at 2690.276 kb on - strand at 2690.276 kb on - strand at 2690.317 kb on - strand, within xthA at 2690.331 kb on - strand, within xthA at 2690.363 kb on - strand, within xthA at 2690.381 kb on - strand, within xthA at 2690.410 kb on - strand, within xthA at 2690.412 kb on + strand, within xthA at 2690.412 kb on + strand at 2690.412 kb on + strand, within xthA at 2690.459 kb on + strand, within xthA at 2690.474 kb on - strand, within xthA at 2690.474 kb on - strand, within xthA at 2690.480 kb on + strand at 2690.534 kb on + strand, within xthA at 2690.562 kb on - strand, within xthA at 2690.576 kb on + strand, within xthA at 2690.586 kb on + strand, within xthA at 2690.612 kb on + strand at 2690.630 kb on - strand, within xthA at 2690.630 kb on - strand, within xthA at 2690.644 kb on + strand, within xthA at 2690.644 kb on + strand, within xthA at 2690.649 kb on + strand, within xthA at 2690.649 kb on + strand, within xthA at 2690.649 kb on + strand, within xthA at 2690.657 kb on - strand, within xthA at 2690.657 kb on - strand, within xthA at 2690.667 kb on - strand, within xthA at 2690.724 kb on - strand, within xthA at 2690.740 kb on + strand, within xthA at 2690.781 kb on - strand, within xthA at 2690.788 kb on - strand, within xthA at 2690.790 kb on + strand, within xthA at 2690.791 kb on - strand, within xthA at 2690.796 kb on - strand, within xthA at 2690.824 kb on + strand, within xthA at 2690.841 kb on + strand, within xthA at 2690.851 kb on + strand, within xthA at 2690.853 kb on - strand, within xthA at 2690.854 kb on - strand, within xthA at 2690.865 kb on - strand, within xthA at 2690.877 kb on - strand, within xthA at 2690.877 kb on - strand, within xthA at 2690.912 kb on - strand, within xthA at 2690.934 kb on + strand at 2690.953 kb on - strand at 2691.005 kb on - strand at 2691.030 kb on + strand at 2691.077 kb on - strand at 2691.086 kb on + strand at 2691.128 kb on - strand at 2691.136 kb on + strand, within Synpcc7942_2608 at 2691.149 kb on - strand, within Synpcc7942_2608 at 2691.169 kb on - strand, within Synpcc7942_2608 at 2691.204 kb on + strand, within Synpcc7942_2608 at 2691.212 kb on - strand, within Synpcc7942_2608 at 2691.212 kb on - strand, within Synpcc7942_2608 at 2691.348 kb on - strand, within Synpcc7942_2608 at 2691.396 kb on - strand at 2691.396 kb on - strand at 2691.429 kb on - strand at 2691.434 kb on + strand at 2691.434 kb on - strand at 2691.442 kb on - strand at 2691.443 kb on - strand at 2691.443 kb on - strand at 2691.455 kb on - strand at 2691.465 kb on - strand at 2691.498 kb on - strand at 2691.506 kb on + strand at 2691.507 kb on - strand at 2691.507 kb on - strand at 2691.509 kb on - strand at 2691.547 kb on - strand at 2691.567 kb on + strand at 2691.576 kb on + strand at 2691.609 kb on - strand at 2691.609 kb on - strand at 2691.617 kb on + strand at 2692.118 kb on + strand at 2692.123 kb on + strand at 2692.123 kb on + strand at 2692.145 kb on - strand at 2692.145 kb on - strand at 2692.145 kb on - strand at 2692.146 kb on + strand at 2692.146 kb on + strand at 2692.146 kb on + strand at 2692.216 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 8:16 remove 2,690,192 + +0.3 2,690,192 + -0.3 2,690,200 - +0.5 2,690,200 - -0.1 2,690,229 + -0.3 2,690,236 + -0.2 2,690,237 - -0.2 2,690,253 + +0.2 2,690,253 + -0.1 2,690,261 - +0.5 2,690,276 - +0.4 2,690,276 - +0.6 2,690,317 - xthA Synpcc7942_2607 0.14 +0.5 2,690,331 - xthA Synpcc7942_2607 0.16 -0.1 2,690,363 - xthA Synpcc7942_2607 0.20 +0.0 2,690,381 - xthA Synpcc7942_2607 0.22 -0.9 2,690,410 - xthA Synpcc7942_2607 0.26 +0.3 2,690,412 + xthA Synpcc7942_2607 0.26 +0.0 2,690,412 + -0.9 2,690,412 + xthA Synpcc7942_2607 0.26 -0.4 2,690,459 + xthA Synpcc7942_2607 0.32 +0.6 2,690,474 - xthA Synpcc7942_2607 0.34 -0.2 2,690,474 - xthA Synpcc7942_2607 0.34 -0.8 2,690,480 + -2.0 2,690,534 + xthA Synpcc7942_2607 0.41 +0.5 2,690,562 - xthA Synpcc7942_2607 0.45 +0.1 2,690,576 + xthA Synpcc7942_2607 0.46 -0.5 2,690,586 + xthA Synpcc7942_2607 0.48 +0.3 2,690,612 + +0.1 2,690,630 - xthA Synpcc7942_2607 0.53 +0.9 2,690,630 - xthA Synpcc7942_2607 0.53 +0.2 2,690,644 + xthA Synpcc7942_2607 0.55 -0.5 2,690,644 + xthA Synpcc7942_2607 0.55 +0.2 2,690,649 + xthA Synpcc7942_2607 0.56 +0.5 2,690,649 + xthA Synpcc7942_2607 0.56 +1.8 2,690,649 + xthA Synpcc7942_2607 0.56 +0.5 2,690,657 - xthA Synpcc7942_2607 0.57 +0.0 2,690,657 - xthA Synpcc7942_2607 0.57 -0.2 2,690,667 - xthA Synpcc7942_2607 0.58 -0.0 2,690,724 - xthA Synpcc7942_2607 0.65 -0.8 2,690,740 + xthA Synpcc7942_2607 0.67 -0.1 2,690,781 - xthA Synpcc7942_2607 0.72 -0.1 2,690,788 - xthA Synpcc7942_2607 0.73 +0.0 2,690,790 + xthA Synpcc7942_2607 0.73 +0.4 2,690,791 - xthA Synpcc7942_2607 0.73 -0.7 2,690,796 - xthA Synpcc7942_2607 0.74 +0.5 2,690,824 + xthA Synpcc7942_2607 0.77 -0.0 2,690,841 + xthA Synpcc7942_2607 0.80 +0.1 2,690,851 + xthA Synpcc7942_2607 0.81 -0.6 2,690,853 - xthA Synpcc7942_2607 0.81 -1.1 2,690,854 - xthA Synpcc7942_2607 0.81 -0.3 2,690,865 - xthA Synpcc7942_2607 0.83 -0.1 2,690,877 - xthA Synpcc7942_2607 0.84 -0.7 2,690,877 - xthA Synpcc7942_2607 0.84 -0.3 2,690,912 - xthA Synpcc7942_2607 0.88 +0.1 2,690,934 + +0.2 2,690,953 - -0.2 2,691,005 - +0.7 2,691,030 + -1.0 2,691,077 - -0.9 2,691,086 + +0.7 2,691,128 - -0.7 2,691,136 + Synpcc7942_2608 0.10 +0.3 2,691,149 - Synpcc7942_2608 0.14 -0.8 2,691,169 - Synpcc7942_2608 0.20 +1.0 2,691,204 + Synpcc7942_2608 0.31 +0.1 2,691,212 - Synpcc7942_2608 0.34 -1.5 2,691,212 - Synpcc7942_2608 0.34 -1.3 2,691,348 - Synpcc7942_2608 0.76 -1.5 2,691,396 - -0.4 2,691,396 - -0.3 2,691,429 - -1.0 2,691,434 + +0.5 2,691,434 - +1.0 2,691,442 - +0.1 2,691,443 - +0.3 2,691,443 - +0.5 2,691,455 - +0.3 2,691,465 - +0.8 2,691,498 - +0.4 2,691,506 + -0.0 2,691,507 - -0.7 2,691,507 - +0.7 2,691,509 - +0.1 2,691,547 - +0.4 2,691,567 + +0.3 2,691,576 + -0.1 2,691,609 - -0.1 2,691,609 - -1.2 2,691,617 + +0.2 2,692,118 + -0.2 2,692,123 + -0.2 2,692,123 + +0.1 2,692,145 - -0.1 2,692,145 - +0.1 2,692,145 - -0.3 2,692,146 + -0.1 2,692,146 + +0.4 2,692,146 + +0.4 2,692,216 - -1.2
Or see this region's nucleotide sequence