Experiment: Flask growth in 60 uE Light-Dark 8:16
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gpsA and Synpcc7942_2522 are separated by 57 nucleotides Synpcc7942_2522 and Synpcc7942_2523 overlap by 2 nucleotides Synpcc7942_2523 and tig are separated by 11 nucleotides
Synpcc7942_2521: gpsA - Nucleotide binding protein, PINc, at 2,602,698 to 2,604,026
gpsA
Synpcc7942_2522: Synpcc7942_2522 - Glycerol-3-phosphate dehydrogenase (NAD(P)+), at 2,604,084 to 2,605,010
_2522
Synpcc7942_2523: Synpcc7942_2523 - hypothetical protein, at 2,605,009 to 2,605,266
_2523
Synpcc7942_2524: tig - trigger factor, at 2,605,278 to 2,606,702
tig
Position (kb)
2605
2606 Strain fitness (log2 ratio)
-1
0
1 at 2604.036 kb on - strand at 2604.051 kb on + strand at 2604.051 kb on + strand at 2604.051 kb on + strand at 2604.051 kb on + strand at 2604.051 kb on + strand at 2604.051 kb on + strand at 2604.051 kb on + strand at 2604.051 kb on + strand at 2604.051 kb on + strand at 2604.064 kb on - strand at 2605.002 kb on - strand at 2605.012 kb on + strand at 2605.013 kb on + strand at 2605.013 kb on + strand at 2605.021 kb on - strand at 2605.026 kb on - strand at 2605.030 kb on + strand at 2605.070 kb on + strand, within Synpcc7942_2523 at 2605.070 kb on + strand, within Synpcc7942_2523 at 2605.083 kb on - strand, within Synpcc7942_2523 at 2605.280 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 8:16 remove 2,604,036 - -1.1 2,604,051 + -0.0 2,604,051 + +0.7 2,604,051 + +0.2 2,604,051 + +0.5 2,604,051 + +0.2 2,604,051 + +0.2 2,604,051 + -0.3 2,604,051 + +0.3 2,604,051 + -0.1 2,604,064 - +0.1 2,605,002 - +1.7 2,605,012 + -0.8 2,605,013 + +0.3 2,605,013 + -1.3 2,605,021 - +0.1 2,605,026 - +1.2 2,605,030 + +0.3 2,605,070 + Synpcc7942_2523 0.24 -0.4 2,605,070 + Synpcc7942_2523 0.24 -0.6 2,605,083 - Synpcc7942_2523 0.29 -1.3 2,605,280 - -0.2
Or see this region's nucleotide sequence