Experiment: Flask growth in 60 uE Light-Dark 8:16
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt slt and manB are separated by 293 nucleotides manB and Synpcc7942_2133 are separated by 2 nucleotides Synpcc7942_2133 and amiA are separated by 182 nucleotides
Synpcc7942_2131: slt - probable soluble lytic transglycosylase, at 2,209,183 to 2,211,255
slt
Synpcc7942_2132: manB - phosphoglucosamine mutase, at 2,211,549 to 2,212,967
manB
Synpcc7942_2133: Synpcc7942_2133 - hypothetical protein, at 2,212,970 to 2,213,476
_2133
Synpcc7942_2134: amiA - Cell wall hydrolase/autolysin, at 2,213,659 to 2,214,708
amiA
Position (kb)
2211
2212
2213 Strain fitness (log2 ratio)
-2
-1
0
1 at 2210.556 kb on - strand, within slt at 2210.559 kb on + strand, within slt at 2210.583 kb on + strand, within slt at 2210.600 kb on + strand, within slt at 2210.620 kb on - strand, within slt at 2210.639 kb on + strand, within slt at 2210.727 kb on + strand, within slt at 2210.735 kb on - strand, within slt at 2210.787 kb on + strand, within slt at 2210.787 kb on + strand, within slt at 2210.797 kb on - strand, within slt at 2210.804 kb on + strand, within slt at 2210.812 kb on - strand, within slt at 2210.814 kb on + strand, within slt at 2210.814 kb on + strand, within slt at 2210.817 kb on - strand, within slt at 2210.834 kb on + strand, within slt at 2210.834 kb on + strand, within slt at 2210.834 kb on + strand, within slt at 2210.834 kb on + strand, within slt at 2210.842 kb on - strand, within slt at 2210.848 kb on + strand, within slt at 2210.872 kb on + strand, within slt at 2210.899 kb on + strand, within slt at 2210.899 kb on + strand, within slt at 2210.899 kb on + strand, within slt at 2210.909 kb on - strand, within slt at 2210.910 kb on + strand, within slt at 2210.915 kb on - strand, within slt at 2210.923 kb on - strand, within slt at 2210.927 kb on + strand, within slt at 2210.932 kb on + strand, within slt at 2210.933 kb on - strand, within slt at 2210.937 kb on + strand, within slt at 2210.937 kb on + strand, within slt at 2210.945 kb on - strand, within slt at 2210.945 kb on - strand, within slt at 2210.945 kb on - strand, within slt at 2210.955 kb on - strand, within slt at 2210.966 kb on + strand, within slt at 2210.971 kb on + strand, within slt at 2210.976 kb on + strand, within slt at 2210.976 kb on + strand, within slt at 2210.976 kb on + strand, within slt at 2210.979 kb on - strand, within slt at 2210.984 kb on - strand, within slt at 2210.984 kb on - strand, within slt at 2210.989 kb on + strand, within slt at 2211.013 kb on + strand, within slt at 2211.031 kb on + strand, within slt at 2211.055 kb on + strand at 2211.055 kb on + strand at 2211.065 kb on + strand at 2211.096 kb on + strand at 2211.096 kb on + strand at 2211.263 kb on + strand at 2211.263 kb on + strand at 2211.271 kb on - strand at 2211.304 kb on - strand at 2211.307 kb on - strand at 2211.310 kb on + strand at 2211.374 kb on - strand at 2211.395 kb on - strand at 2211.395 kb on - strand at 2211.422 kb on + strand at 2211.422 kb on + strand at 2211.423 kb on - strand at 2211.423 kb on - strand at 2211.442 kb on + strand at 2211.450 kb on - strand at 2211.453 kb on - strand at 2211.465 kb on + strand at 2211.495 kb on - strand at 2211.534 kb on + strand at 2211.540 kb on - strand at 2211.540 kb on - strand at 2211.542 kb on - strand at 2211.547 kb on - strand at 2211.591 kb on + strand at 2211.599 kb on - strand at 2211.626 kb on + strand at 2211.730 kb on - strand, within manB at 2211.730 kb on - strand, within manB at 2211.799 kb on + strand, within manB at 2211.891 kb on - strand, within manB at 2212.053 kb on + strand, within manB at 2212.204 kb on + strand, within manB at 2212.370 kb on + strand, within manB at 2212.605 kb on - strand, within manB at 2212.794 kb on - strand, within manB at 2212.799 kb on - strand, within manB at 2212.964 kb on - strand at 2212.968 kb on + strand at 2212.969 kb on - strand at 2212.969 kb on - strand at 2212.997 kb on + strand at 2213.007 kb on + strand at 2213.013 kb on + strand at 2213.015 kb on - strand at 2213.057 kb on + strand, within Synpcc7942_2133 at 2213.060 kb on - strand, within Synpcc7942_2133 at 2213.063 kb on + strand, within Synpcc7942_2133 at 2213.071 kb on - strand, within Synpcc7942_2133 at 2213.093 kb on + strand, within Synpcc7942_2133 at 2213.175 kb on + strand, within Synpcc7942_2133 at 2213.175 kb on + strand, within Synpcc7942_2133 at 2213.183 kb on - strand, within Synpcc7942_2133 at 2213.195 kb on - strand, within Synpcc7942_2133 at 2213.203 kb on - strand, within Synpcc7942_2133 at 2213.203 kb on - strand, within Synpcc7942_2133 at 2213.207 kb on - strand, within Synpcc7942_2133 at 2213.246 kb on + strand, within Synpcc7942_2133 at 2213.259 kb on - strand, within Synpcc7942_2133 at 2213.269 kb on - strand, within Synpcc7942_2133 at 2213.272 kb on - strand, within Synpcc7942_2133 at 2213.276 kb on - strand, within Synpcc7942_2133 at 2213.281 kb on - strand, within Synpcc7942_2133 at 2213.375 kb on + strand, within Synpcc7942_2133 at 2213.375 kb on + strand, within Synpcc7942_2133 at 2213.375 kb on + strand, within Synpcc7942_2133 at 2213.375 kb on + strand, within Synpcc7942_2133 at 2213.375 kb on + strand, within Synpcc7942_2133 at 2213.380 kb on + strand, within Synpcc7942_2133 at 2213.383 kb on - strand, within Synpcc7942_2133 at 2213.383 kb on - strand, within Synpcc7942_2133 at 2213.383 kb on - strand, within Synpcc7942_2133 at 2213.384 kb on + strand, within Synpcc7942_2133 at 2213.385 kb on + strand, within Synpcc7942_2133 at 2213.385 kb on + strand, within Synpcc7942_2133 at 2213.393 kb on - strand, within Synpcc7942_2133 at 2213.393 kb on - strand, within Synpcc7942_2133 at 2213.393 kb on - strand, within Synpcc7942_2133 at 2213.405 kb on + strand, within Synpcc7942_2133 at 2213.423 kb on - strand, within Synpcc7942_2133 at 2213.425 kb on + strand, within Synpcc7942_2133 at 2213.437 kb on - strand at 2213.444 kb on - strand at 2213.507 kb on - strand at 2213.507 kb on - strand at 2213.525 kb on - strand at 2213.545 kb on + strand at 2213.590 kb on + strand at 2213.590 kb on - strand at 2213.598 kb on - strand at 2213.605 kb on - strand at 2213.624 kb on - strand at 2213.624 kb on - strand at 2213.639 kb on + strand at 2213.639 kb on + strand at 2213.647 kb on - strand at 2213.647 kb on - strand at 2213.647 kb on - strand at 2213.650 kb on + strand at 2213.652 kb on - strand at 2213.682 kb on - strand at 2213.709 kb on - strand at 2213.752 kb on - strand at 2213.753 kb on - strand at 2213.787 kb on - strand, within amiA at 2213.842 kb on - strand, within amiA at 2213.854 kb on + strand, within amiA at 2213.854 kb on + strand, within amiA at 2213.862 kb on - strand, within amiA at 2213.877 kb on - strand, within amiA at 2213.877 kb on - strand, within amiA at 2213.877 kb on - strand, within amiA at 2213.877 kb on - strand, within amiA at 2213.897 kb on - strand, within amiA at 2213.918 kb on + strand, within amiA at 2213.922 kb on - strand, within amiA at 2213.929 kb on + strand, within amiA at 2213.934 kb on + strand, within amiA at 2213.953 kb on - strand, within amiA
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 8:16 remove 2,210,556 - slt Synpcc7942_2131 0.66 +0.3 2,210,559 + slt Synpcc7942_2131 0.66 -0.5 2,210,583 + slt Synpcc7942_2131 0.68 +0.6 2,210,600 + slt Synpcc7942_2131 0.68 +0.2 2,210,620 - slt Synpcc7942_2131 0.69 -1.1 2,210,639 + slt Synpcc7942_2131 0.70 +0.7 2,210,727 + slt Synpcc7942_2131 0.74 -0.2 2,210,735 - slt Synpcc7942_2131 0.75 +0.1 2,210,787 + slt Synpcc7942_2131 0.77 -0.5 2,210,787 + slt Synpcc7942_2131 0.77 -0.6 2,210,797 - slt Synpcc7942_2131 0.78 +0.2 2,210,804 + slt Synpcc7942_2131 0.78 +1.3 2,210,812 - slt Synpcc7942_2131 0.79 -0.2 2,210,814 + slt Synpcc7942_2131 0.79 +0.2 2,210,814 + slt Synpcc7942_2131 0.79 -0.2 2,210,817 - slt Synpcc7942_2131 0.79 -0.4 2,210,834 + slt Synpcc7942_2131 0.80 +0.6 2,210,834 + slt Synpcc7942_2131 0.80 +0.0 2,210,834 + slt Synpcc7942_2131 0.80 -0.3 2,210,834 + slt Synpcc7942_2131 0.80 +0.5 2,210,842 - slt Synpcc7942_2131 0.80 +0.8 2,210,848 + slt Synpcc7942_2131 0.80 -0.5 2,210,872 + slt Synpcc7942_2131 0.81 +0.1 2,210,899 + slt Synpcc7942_2131 0.83 -1.0 2,210,899 + slt Synpcc7942_2131 0.83 -0.2 2,210,899 + slt Synpcc7942_2131 0.83 +0.6 2,210,909 - slt Synpcc7942_2131 0.83 +0.5 2,210,910 + slt Synpcc7942_2131 0.83 +0.8 2,210,915 - slt Synpcc7942_2131 0.84 -1.3 2,210,923 - slt Synpcc7942_2131 0.84 +0.0 2,210,927 + slt Synpcc7942_2131 0.84 -0.9 2,210,932 + slt Synpcc7942_2131 0.84 -1.1 2,210,933 - slt Synpcc7942_2131 0.84 +0.7 2,210,937 + slt Synpcc7942_2131 0.85 +0.3 2,210,937 + slt Synpcc7942_2131 0.85 +0.7 2,210,945 - slt Synpcc7942_2131 0.85 +0.2 2,210,945 - slt Synpcc7942_2131 0.85 -0.6 2,210,945 - slt Synpcc7942_2131 0.85 -0.3 2,210,955 - slt Synpcc7942_2131 0.85 +0.1 2,210,966 + slt Synpcc7942_2131 0.86 +0.0 2,210,971 + slt Synpcc7942_2131 0.86 -0.2 2,210,976 + slt Synpcc7942_2131 0.86 -1.3 2,210,976 + slt Synpcc7942_2131 0.86 +0.0 2,210,976 + slt Synpcc7942_2131 0.86 -0.3 2,210,979 - slt Synpcc7942_2131 0.87 +0.5 2,210,984 - slt Synpcc7942_2131 0.87 -0.1 2,210,984 - slt Synpcc7942_2131 0.87 -0.4 2,210,989 + slt Synpcc7942_2131 0.87 -1.6 2,211,013 + slt Synpcc7942_2131 0.88 +0.1 2,211,031 + slt Synpcc7942_2131 0.89 +0.1 2,211,055 + -0.1 2,211,055 + -0.9 2,211,065 + +0.4 2,211,096 + -0.2 2,211,096 + +0.3 2,211,263 + -0.0 2,211,263 + +0.3 2,211,271 - -1.0 2,211,304 - -0.2 2,211,307 - -0.9 2,211,310 + +0.4 2,211,374 - -0.5 2,211,395 - +0.2 2,211,395 - +0.1 2,211,422 + +0.1 2,211,422 + +0.1 2,211,423 - +0.1 2,211,423 - -0.2 2,211,442 + -0.7 2,211,450 - -0.4 2,211,453 - -0.2 2,211,465 + -0.6 2,211,495 - -0.1 2,211,534 + +0.2 2,211,540 - -0.5 2,211,540 - -0.5 2,211,542 - -0.2 2,211,547 - +0.0 2,211,591 + -0.5 2,211,599 - -0.3 2,211,626 + -0.1 2,211,730 - manB Synpcc7942_2132 0.13 +0.1 2,211,730 - manB Synpcc7942_2132 0.13 -1.7 2,211,799 + manB Synpcc7942_2132 0.18 -0.5 2,211,891 - manB Synpcc7942_2132 0.24 +0.1 2,212,053 + manB Synpcc7942_2132 0.36 -1.1 2,212,204 + manB Synpcc7942_2132 0.46 -0.3 2,212,370 + manB Synpcc7942_2132 0.58 -2.5 2,212,605 - manB Synpcc7942_2132 0.74 -0.7 2,212,794 - manB Synpcc7942_2132 0.88 -1.4 2,212,799 - manB Synpcc7942_2132 0.88 -0.9 2,212,964 - +0.6 2,212,968 + +0.3 2,212,969 - +0.5 2,212,969 - -0.0 2,212,997 + +0.4 2,213,007 + +0.7 2,213,013 + +0.4 2,213,015 - +0.1 2,213,057 + Synpcc7942_2133 0.17 +0.1 2,213,060 - Synpcc7942_2133 0.18 -0.6 2,213,063 + Synpcc7942_2133 0.18 -0.2 2,213,071 - Synpcc7942_2133 0.20 +0.9 2,213,093 + Synpcc7942_2133 0.24 +0.3 2,213,175 + Synpcc7942_2133 0.40 +0.4 2,213,175 + Synpcc7942_2133 0.40 -0.4 2,213,183 - Synpcc7942_2133 0.42 -2.1 2,213,195 - Synpcc7942_2133 0.44 +0.9 2,213,203 - Synpcc7942_2133 0.46 -1.2 2,213,203 - Synpcc7942_2133 0.46 +0.3 2,213,207 - Synpcc7942_2133 0.47 -0.2 2,213,246 + Synpcc7942_2133 0.54 -0.2 2,213,259 - Synpcc7942_2133 0.57 +0.7 2,213,269 - Synpcc7942_2133 0.59 +0.5 2,213,272 - Synpcc7942_2133 0.60 +0.3 2,213,276 - Synpcc7942_2133 0.60 +0.9 2,213,281 - Synpcc7942_2133 0.61 +0.0 2,213,375 + Synpcc7942_2133 0.80 -0.6 2,213,375 + Synpcc7942_2133 0.80 +0.2 2,213,375 + Synpcc7942_2133 0.80 -0.4 2,213,375 + Synpcc7942_2133 0.80 +0.3 2,213,375 + Synpcc7942_2133 0.80 +0.7 2,213,380 + Synpcc7942_2133 0.81 +0.7 2,213,383 - Synpcc7942_2133 0.81 -0.3 2,213,383 - Synpcc7942_2133 0.81 -1.0 2,213,383 - Synpcc7942_2133 0.81 -0.6 2,213,384 + Synpcc7942_2133 0.82 +0.5 2,213,385 + Synpcc7942_2133 0.82 +0.2 2,213,385 + Synpcc7942_2133 0.82 -0.3 2,213,393 - Synpcc7942_2133 0.83 +0.3 2,213,393 - Synpcc7942_2133 0.83 +0.7 2,213,393 - Synpcc7942_2133 0.83 -0.4 2,213,405 + Synpcc7942_2133 0.86 +0.8 2,213,423 - Synpcc7942_2133 0.89 +0.4 2,213,425 + Synpcc7942_2133 0.90 +0.4 2,213,437 - -0.3 2,213,444 - +0.3 2,213,507 - +0.3 2,213,507 - +0.1 2,213,525 - -0.8 2,213,545 + -0.5 2,213,590 + +1.1 2,213,590 - -0.3 2,213,598 - +0.3 2,213,605 - +0.8 2,213,624 - -0.1 2,213,624 - +0.7 2,213,639 + -0.3 2,213,639 + +0.4 2,213,647 - -0.1 2,213,647 - +1.3 2,213,647 - +0.8 2,213,650 + +0.0 2,213,652 - +1.2 2,213,682 - +0.4 2,213,709 - +1.0 2,213,752 - +0.3 2,213,753 - -1.1 2,213,787 - amiA Synpcc7942_2134 0.12 -0.4 2,213,842 - amiA Synpcc7942_2134 0.17 +0.8 2,213,854 + amiA Synpcc7942_2134 0.19 +0.2 2,213,854 + amiA Synpcc7942_2134 0.19 +0.0 2,213,862 - amiA Synpcc7942_2134 0.19 -0.0 2,213,877 - amiA Synpcc7942_2134 0.21 +0.0 2,213,877 - amiA Synpcc7942_2134 0.21 +0.4 2,213,877 - amiA Synpcc7942_2134 0.21 +0.5 2,213,877 - amiA Synpcc7942_2134 0.21 +0.3 2,213,897 - amiA Synpcc7942_2134 0.23 +0.2 2,213,918 + amiA Synpcc7942_2134 0.25 +0.5 2,213,922 - amiA Synpcc7942_2134 0.25 +0.2 2,213,929 + amiA Synpcc7942_2134 0.26 +0.1 2,213,934 + amiA Synpcc7942_2134 0.26 -0.2 2,213,953 - amiA Synpcc7942_2134 0.28 -0.3
Or see this region's nucleotide sequence