Experiment: Flask growth in 60 uE Light-Dark 8:16
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phoU and psaM are separated by 123 nucleotides psaM and Synpcc7942_1913 are separated by 156 nucleotides Synpcc7942_1913 and Synpcc7942_1914 are separated by 35 nucleotides
Synpcc7942_1912: phoU - phosphate uptake regulator, PhoU, at 1,988,931 to 1,989,605
phoU
Synpcc7942_1912a: psaM - photosystem I reaction center subunit XII, at 1,989,729 to 1,989,818
psaM
Synpcc7942_1913: Synpcc7942_1913 - hypothetical protein, at 1,989,975 to 1,990,358
_1913
Synpcc7942_1914: Synpcc7942_1914 - hypothetical protein, at 1,990,394 to 1,990,852
_1914
Position (kb)
1989
1990 Strain fitness (log2 ratio)
-2
-1
0
1 at 1988.729 kb on - strand at 1988.772 kb on + strand at 1988.798 kb on - strand at 1988.827 kb on - strand at 1988.864 kb on + strand at 1988.864 kb on + strand at 1988.869 kb on + strand at 1988.880 kb on + strand at 1988.880 kb on + strand at 1988.882 kb on + strand at 1988.900 kb on + strand at 1988.900 kb on + strand at 1988.908 kb on + strand at 1988.908 kb on - strand at 1988.937 kb on - strand at 1989.592 kb on + strand at 1989.612 kb on + strand at 1989.612 kb on + strand at 1989.743 kb on + strand, within psaM at 1989.759 kb on + strand, within psaM at 1989.761 kb on - strand, within psaM at 1989.813 kb on + strand at 1989.829 kb on + strand at 1989.837 kb on - strand at 1989.869 kb on - strand at 1989.949 kb on + strand at 1990.073 kb on + strand, within Synpcc7942_1913 at 1990.075 kb on - strand, within Synpcc7942_1913 at 1990.359 kb on - strand at 1990.380 kb on - strand at 1990.419 kb on - strand at 1990.453 kb on - strand, within Synpcc7942_1914 at 1990.477 kb on + strand, within Synpcc7942_1914 at 1990.526 kb on + strand, within Synpcc7942_1914 at 1990.578 kb on + strand, within Synpcc7942_1914 at 1990.586 kb on - strand, within Synpcc7942_1914 at 1990.596 kb on + strand, within Synpcc7942_1914 at 1990.604 kb on - strand, within Synpcc7942_1914 at 1990.618 kb on - strand, within Synpcc7942_1914 at 1990.629 kb on - strand, within Synpcc7942_1914 at 1990.629 kb on - strand, within Synpcc7942_1914 at 1990.641 kb on - strand, within Synpcc7942_1914 at 1990.648 kb on + strand, within Synpcc7942_1914 at 1990.648 kb on + strand, within Synpcc7942_1914 at 1990.651 kb on - strand, within Synpcc7942_1914 at 1990.656 kb on - strand at 1990.656 kb on - strand, within Synpcc7942_1914 at 1990.680 kb on - strand, within Synpcc7942_1914 at 1990.680 kb on - strand, within Synpcc7942_1914 at 1990.713 kb on + strand, within Synpcc7942_1914 at 1990.735 kb on - strand, within Synpcc7942_1914 at 1990.788 kb on - strand, within Synpcc7942_1914 at 1990.803 kb on + strand, within Synpcc7942_1914 at 1990.811 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 8:16 remove 1,988,729 - +1.1 1,988,772 + -1.3 1,988,798 - -1.0 1,988,827 - -1.1 1,988,864 + -0.6 1,988,864 + +1.3 1,988,869 + -0.5 1,988,880 + -0.9 1,988,880 + +0.2 1,988,882 + -1.8 1,988,900 + -0.2 1,988,900 + -0.1 1,988,908 + -0.9 1,988,908 - -0.8 1,988,937 - -0.2 1,989,592 + +0.5 1,989,612 + +1.5 1,989,612 + +0.5 1,989,743 + psaM Synpcc7942_1912a 0.16 +0.2 1,989,759 + psaM Synpcc7942_1912a 0.33 -1.3 1,989,761 - psaM Synpcc7942_1912a 0.36 -0.2 1,989,813 + -2.1 1,989,829 + +0.2 1,989,837 - -0.6 1,989,869 - -0.2 1,989,949 + +0.1 1,990,073 + Synpcc7942_1913 0.26 -0.1 1,990,075 - Synpcc7942_1913 0.26 -0.8 1,990,359 - +0.1 1,990,380 - +0.3 1,990,419 - +0.2 1,990,453 - Synpcc7942_1914 0.13 -0.5 1,990,477 + Synpcc7942_1914 0.18 +0.2 1,990,526 + Synpcc7942_1914 0.29 +0.1 1,990,578 + Synpcc7942_1914 0.40 +0.3 1,990,586 - Synpcc7942_1914 0.42 -0.1 1,990,596 + Synpcc7942_1914 0.44 -0.1 1,990,604 - Synpcc7942_1914 0.46 -0.6 1,990,618 - Synpcc7942_1914 0.49 +0.1 1,990,629 - Synpcc7942_1914 0.51 -0.8 1,990,629 - Synpcc7942_1914 0.51 -1.7 1,990,641 - Synpcc7942_1914 0.54 +0.4 1,990,648 + Synpcc7942_1914 0.55 -0.3 1,990,648 + Synpcc7942_1914 0.55 -1.4 1,990,651 - Synpcc7942_1914 0.56 +0.7 1,990,656 - -0.0 1,990,656 - Synpcc7942_1914 0.57 -0.2 1,990,680 - Synpcc7942_1914 0.62 -0.3 1,990,680 - Synpcc7942_1914 0.62 -0.7 1,990,713 + Synpcc7942_1914 0.69 +0.4 1,990,735 - Synpcc7942_1914 0.74 -0.5 1,990,788 - Synpcc7942_1914 0.86 +0.1 1,990,803 + Synpcc7942_1914 0.89 -0.7 1,990,811 - -0.6
Or see this region's nucleotide sequence