Experiment: Flask growth in 60 uE Light-Dark 8:16
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt carB and Synpcc7942_0712 are separated by 175 nucleotides Synpcc7942_0712 and ispG are separated by 99 nucleotides
Synpcc7942_0711: carB - carbamoyl phosphate synthase large subunit, at 701,604 to 704,897
carB
Synpcc7942_0712: Synpcc7942_0712 - C-terminal processing peptidase-2. Serine peptidase. MEROPS family S41A, at 705,073 to 706,350
_0712
Synpcc7942_0713: ispG - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, at 706,450 to 707,673
ispG
Position (kb) 705
706
707 Strain fitness (log2 ratio) -1
0
1 at 704.938 kb on - strand at 705.135 kb on - strand at 705.186 kb on + strand at 705.193 kb on + strand at 705.254 kb on + strand, within Synpcc7942_0712 at 705.354 kb on - strand, within Synpcc7942_0712 at 705.354 kb on - strand, within Synpcc7942_0712 at 705.354 kb on - strand, within Synpcc7942_0712 at 705.447 kb on + strand, within Synpcc7942_0712 at 705.455 kb on - strand, within Synpcc7942_0712 at 705.462 kb on - strand, within Synpcc7942_0712 at 705.553 kb on + strand, within Synpcc7942_0712 at 705.618 kb on + strand, within Synpcc7942_0712 at 705.945 kb on + strand, within Synpcc7942_0712 at 705.969 kb on - strand, within Synpcc7942_0712 at 706.004 kb on - strand, within Synpcc7942_0712 at 706.078 kb on + strand, within Synpcc7942_0712 at 706.086 kb on - strand, within Synpcc7942_0712 at 706.086 kb on - strand, within Synpcc7942_0712 at 706.091 kb on + strand, within Synpcc7942_0712 at 706.099 kb on - strand, within Synpcc7942_0712 at 706.101 kb on - strand, within Synpcc7942_0712 at 706.151 kb on + strand, within Synpcc7942_0712 at 706.248 kb on + strand at 706.282 kb on + strand at 706.352 kb on + strand at 706.360 kb on - strand at 706.387 kb on + strand at 706.387 kb on + strand at 706.387 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 8:16 remove 704,938 - -0.6 705,135 - -0.5 705,186 + -0.4 705,193 + -0.2 705,254 + Synpcc7942_0712 0.14 -0.8 705,354 - Synpcc7942_0712 0.22 -0.2 705,354 - Synpcc7942_0712 0.22 -0.8 705,354 - Synpcc7942_0712 0.22 -0.3 705,447 + Synpcc7942_0712 0.29 -0.4 705,455 - Synpcc7942_0712 0.30 -1.3 705,462 - Synpcc7942_0712 0.30 -1.0 705,553 + Synpcc7942_0712 0.38 -0.6 705,618 + Synpcc7942_0712 0.43 -0.8 705,945 + Synpcc7942_0712 0.68 -0.6 705,969 - Synpcc7942_0712 0.70 -0.8 706,004 - Synpcc7942_0712 0.73 -1.5 706,078 + Synpcc7942_0712 0.79 -0.8 706,086 - Synpcc7942_0712 0.79 -0.3 706,086 - Synpcc7942_0712 0.79 -0.4 706,091 + Synpcc7942_0712 0.80 -1.0 706,099 - Synpcc7942_0712 0.80 -0.5 706,101 - Synpcc7942_0712 0.80 +0.7 706,151 + Synpcc7942_0712 0.84 -0.3 706,248 + -1.2 706,282 + +0.0 706,352 + +0.4 706,360 - -0.7 706,387 + -0.1 706,387 + +0.2 706,387 + +0.1
Or see this region's nucleotide sequence