Experiment: Flask growth in 60 uE Light-Dark 8:16
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiG and Synpcc7942_0577 are separated by 83 nucleotides Synpcc7942_0577 and sqdB are separated by 93 nucleotides sqdB and sqdX are separated by 15 nucleotides
Synpcc7942_0576: thiG - thiazole synthase, at 557,200 to 558,060
thiG
Synpcc7942_0577: Synpcc7942_0577 - hypothetical protein, at 558,144 to 558,314
_0577
Synpcc7942_0578: sqdB - UDP-sulfoquinovose synthase, at 558,408 to 559,616
sqdB
Synpcc7942_0579: sqdX - sulfolipid sulfoquinovosyldiacylglycerol biosynthesis protein, at 559,632 to 560,765
sqdX
Position (kb) 558
559
560 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 558.053 kb on + strand at 558.084 kb on + strand at 558.095 kb on + strand at 558.101 kb on - strand at 558.108 kb on - strand at 558.108 kb on - strand at 558.108 kb on - strand at 558.114 kb on - strand at 558.116 kb on + strand at 558.118 kb on + strand at 558.118 kb on + strand at 558.118 kb on + strand at 558.119 kb on - strand at 558.121 kb on + strand at 558.126 kb on - strand at 558.129 kb on - strand at 558.129 kb on - strand at 558.129 kb on - strand at 558.131 kb on - strand at 558.136 kb on - strand at 558.147 kb on + strand at 558.147 kb on + strand at 558.147 kb on + strand at 558.154 kb on - strand at 558.155 kb on - strand at 558.155 kb on - strand at 558.155 kb on - strand at 558.157 kb on - strand at 558.165 kb on + strand, within Synpcc7942_0577 at 558.173 kb on + strand, within Synpcc7942_0577 at 558.173 kb on - strand, within Synpcc7942_0577 at 558.202 kb on - strand, within Synpcc7942_0577 at 558.210 kb on + strand, within Synpcc7942_0577 at 558.220 kb on + strand, within Synpcc7942_0577 at 558.220 kb on + strand, within Synpcc7942_0577 at 558.223 kb on - strand, within Synpcc7942_0577 at 558.228 kb on - strand, within Synpcc7942_0577 at 558.228 kb on - strand at 558.256 kb on + strand, within Synpcc7942_0577 at 558.282 kb on + strand, within Synpcc7942_0577 at 558.311 kb on + strand at 558.321 kb on - strand at 558.324 kb on - strand at 558.332 kb on + strand at 558.378 kb on + strand at 558.383 kb on + strand at 558.406 kb on - strand at 558.545 kb on - strand, within sqdB at 558.650 kb on + strand, within sqdB at 558.725 kb on + strand, within sqdB at 558.766 kb on - strand, within sqdB at 558.791 kb on - strand, within sqdB at 558.849 kb on - strand, within sqdB at 558.978 kb on - strand, within sqdB at 559.146 kb on + strand, within sqdB at 559.154 kb on - strand, within sqdB at 559.217 kb on - strand, within sqdB at 559.311 kb on - strand, within sqdB at 559.332 kb on - strand, within sqdB at 559.470 kb on + strand, within sqdB at 559.720 kb on + strand at 559.822 kb on - strand, within sqdX at 559.882 kb on - strand, within sqdX at 559.911 kb on + strand, within sqdX at 559.943 kb on + strand, within sqdX at 560.000 kb on + strand, within sqdX at 560.077 kb on + strand, within sqdX at 560.129 kb on + strand, within sqdX at 560.260 kb on - strand, within sqdX at 560.309 kb on + strand, within sqdX at 560.329 kb on + strand, within sqdX at 560.358 kb on - strand, within sqdX at 560.429 kb on - strand, within sqdX at 560.615 kb on + strand, within sqdX
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 8:16 remove 558,053 + -0.1 558,084 + -0.2 558,095 + -0.5 558,101 - +0.2 558,108 - -1.0 558,108 - +0.3 558,108 - -0.9 558,114 - -0.0 558,116 + +0.1 558,118 + -2.9 558,118 + -0.5 558,118 + +0.5 558,119 - +0.1 558,121 + +0.3 558,126 - -1.3 558,129 - +0.5 558,129 - -0.5 558,129 - +0.8 558,131 - +0.4 558,136 - +0.1 558,147 + +0.0 558,147 + +0.4 558,147 + +0.2 558,154 - +0.4 558,155 - +0.2 558,155 - -0.1 558,155 - +0.7 558,157 - +0.1 558,165 + Synpcc7942_0577 0.12 -0.0 558,173 + Synpcc7942_0577 0.17 -0.4 558,173 - Synpcc7942_0577 0.17 +0.3 558,202 - Synpcc7942_0577 0.34 -0.3 558,210 + Synpcc7942_0577 0.39 +1.5 558,220 + Synpcc7942_0577 0.44 -0.1 558,220 + Synpcc7942_0577 0.44 +0.1 558,223 - Synpcc7942_0577 0.46 +0.6 558,228 - Synpcc7942_0577 0.49 +0.4 558,228 - +0.3 558,256 + Synpcc7942_0577 0.65 -0.3 558,282 + Synpcc7942_0577 0.81 -0.1 558,311 + -0.6 558,321 - +0.3 558,324 - -1.4 558,332 + +0.7 558,378 + -0.7 558,383 + -0.3 558,406 - -1.5 558,545 - sqdB Synpcc7942_0578 0.11 -2.0 558,650 + sqdB Synpcc7942_0578 0.20 -1.5 558,725 + sqdB Synpcc7942_0578 0.26 -0.9 558,766 - sqdB Synpcc7942_0578 0.30 -0.0 558,791 - sqdB Synpcc7942_0578 0.32 -1.6 558,849 - sqdB Synpcc7942_0578 0.36 -0.8 558,978 - sqdB Synpcc7942_0578 0.47 -2.4 559,146 + sqdB Synpcc7942_0578 0.61 -1.3 559,154 - sqdB Synpcc7942_0578 0.62 -0.3 559,217 - sqdB Synpcc7942_0578 0.67 -2.0 559,311 - sqdB Synpcc7942_0578 0.75 -0.5 559,332 - sqdB Synpcc7942_0578 0.76 -2.6 559,470 + sqdB Synpcc7942_0578 0.88 -1.3 559,720 + -2.6 559,822 - sqdX Synpcc7942_0579 0.17 -1.5 559,882 - sqdX Synpcc7942_0579 0.22 -0.1 559,911 + sqdX Synpcc7942_0579 0.25 -2.3 559,943 + sqdX Synpcc7942_0579 0.27 -1.9 560,000 + sqdX Synpcc7942_0579 0.32 -1.0 560,077 + sqdX Synpcc7942_0579 0.39 -0.0 560,129 + sqdX Synpcc7942_0579 0.44 -0.4 560,260 - sqdX Synpcc7942_0579 0.55 -2.1 560,309 + sqdX Synpcc7942_0579 0.60 -1.3 560,329 + sqdX Synpcc7942_0579 0.61 -1.1 560,358 - sqdX Synpcc7942_0579 0.64 -3.0 560,429 - sqdX Synpcc7942_0579 0.70 -1.4 560,615 + sqdX Synpcc7942_0579 0.87 -1.5
Or see this region's nucleotide sequence