Experiment: Flask growth in 60 uE Light-Dark 8:16
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0528 and nagB overlap by 36 nucleotides nagB and Synpcc7942_0530 are separated by 3 nucleotides Synpcc7942_0530 and Synpcc7942_0531 are separated by 24 nucleotides
Synpcc7942_0528: Synpcc7942_0528 - Lipopolysaccharide biosynthesis proteins LPS, at 512,589 to 513,578
_0528
Synpcc7942_0529: nagB - 6-phosphogluconolactonase, at 513,543 to 514,331
nagB
Synpcc7942_0530: Synpcc7942_0530 - 4Fe-4S cluster binding, at 514,335 to 515,288
_0530
Synpcc7942_0531: Synpcc7942_0531 - TPR repeat, at 515,313 to 516,176
_0531
Position (kb) 513
514
515 Strain fitness (log2 ratio) -2
-1
0
1
2 at 512.563 kb on + strand at 512.569 kb on + strand at 512.578 kb on + strand at 512.599 kb on - strand at 512.601 kb on + strand at 512.607 kb on + strand at 512.607 kb on + strand at 512.607 kb on + strand at 512.609 kb on - strand at 512.609 kb on - strand at 512.610 kb on - strand at 512.610 kb on - strand at 512.610 kb on - strand at 512.626 kb on - strand at 512.629 kb on - strand at 512.658 kb on + strand at 512.725 kb on - strand, within Synpcc7942_0528 at 512.757 kb on + strand, within Synpcc7942_0528 at 512.799 kb on - strand, within Synpcc7942_0528 at 512.812 kb on + strand, within Synpcc7942_0528 at 512.814 kb on + strand, within Synpcc7942_0528 at 512.879 kb on - strand, within Synpcc7942_0528 at 512.921 kb on + strand, within Synpcc7942_0528 at 512.943 kb on + strand, within Synpcc7942_0528 at 512.951 kb on - strand, within Synpcc7942_0528 at 512.957 kb on - strand, within Synpcc7942_0528 at 512.962 kb on + strand, within Synpcc7942_0528 at 512.970 kb on - strand, within Synpcc7942_0528 at 512.973 kb on + strand, within Synpcc7942_0528 at 512.983 kb on + strand, within Synpcc7942_0528 at 513.024 kb on + strand, within Synpcc7942_0528 at 513.029 kb on - strand, within Synpcc7942_0528 at 513.049 kb on + strand, within Synpcc7942_0528 at 513.160 kb on - strand, within Synpcc7942_0528 at 513.162 kb on - strand, within Synpcc7942_0528 at 513.171 kb on - strand, within Synpcc7942_0528 at 513.182 kb on + strand, within Synpcc7942_0528 at 513.203 kb on + strand, within Synpcc7942_0528 at 513.249 kb on + strand, within Synpcc7942_0528 at 513.251 kb on + strand, within Synpcc7942_0528 at 513.257 kb on - strand, within Synpcc7942_0528 at 513.262 kb on - strand, within Synpcc7942_0528 at 513.378 kb on + strand, within Synpcc7942_0528 at 513.386 kb on - strand, within Synpcc7942_0528 at 513.386 kb on - strand, within Synpcc7942_0528 at 513.386 kb on - strand, within Synpcc7942_0528 at 513.386 kb on - strand, within Synpcc7942_0528 at 513.416 kb on + strand, within Synpcc7942_0528 at 513.418 kb on - strand, within Synpcc7942_0528 at 513.447 kb on + strand, within Synpcc7942_0528 at 513.460 kb on - strand, within Synpcc7942_0528 at 513.476 kb on - strand, within Synpcc7942_0528 at 513.488 kb on + strand at 513.496 kb on - strand at 513.499 kb on + strand at 513.512 kb on + strand at 513.516 kb on + strand at 513.516 kb on + strand at 513.519 kb on - strand at 513.520 kb on - strand at 513.524 kb on - strand at 513.524 kb on - strand at 513.533 kb on + strand at 513.547 kb on - strand at 513.559 kb on - strand at 513.561 kb on + strand at 513.564 kb on - strand at 513.564 kb on - strand at 513.575 kb on + strand at 513.581 kb on + strand at 513.583 kb on - strand at 513.593 kb on - strand at 513.593 kb on - strand at 513.596 kb on + strand at 513.596 kb on + strand at 513.605 kb on - strand at 513.630 kb on + strand, within nagB at 513.671 kb on - strand, within nagB at 513.693 kb on - strand, within nagB at 513.723 kb on - strand, within nagB at 513.735 kb on + strand, within nagB at 513.754 kb on - strand, within nagB at 513.765 kb on + strand, within nagB at 513.766 kb on - strand, within nagB at 513.775 kb on + strand, within nagB at 513.799 kb on + strand, within nagB at 513.814 kb on - strand, within nagB at 513.816 kb on + strand, within nagB at 513.856 kb on + strand, within nagB at 513.858 kb on - strand, within nagB at 513.908 kb on + strand, within nagB at 513.916 kb on - strand, within nagB at 513.940 kb on - strand, within nagB at 513.990 kb on + strand, within nagB at 513.990 kb on + strand, within nagB at 514.004 kb on - strand, within nagB at 514.019 kb on - strand, within nagB at 514.027 kb on + strand, within nagB at 514.067 kb on - strand, within nagB at 514.080 kb on + strand, within nagB at 514.088 kb on - strand, within nagB at 514.088 kb on - strand, within nagB at 514.094 kb on + strand, within nagB at 514.247 kb on - strand, within nagB at 514.272 kb on - strand at 514.289 kb on + strand at 514.297 kb on - strand at 514.330 kb on + strand at 514.358 kb on + strand at 514.361 kb on - strand at 514.361 kb on - strand at 514.366 kb on - strand at 514.366 kb on - strand at 514.366 kb on - strand at 514.366 kb on - strand at 514.366 kb on - strand at 514.366 kb on - strand at 514.392 kb on - strand at 514.458 kb on + strand, within Synpcc7942_0530 at 514.458 kb on + strand, within Synpcc7942_0530 at 514.458 kb on + strand, within Synpcc7942_0530 at 514.458 kb on + strand, within Synpcc7942_0530 at 514.458 kb on + strand, within Synpcc7942_0530 at 514.466 kb on - strand, within Synpcc7942_0530 at 514.466 kb on - strand, within Synpcc7942_0530 at 514.466 kb on - strand, within Synpcc7942_0530 at 514.466 kb on - strand, within Synpcc7942_0530 at 514.466 kb on - strand, within Synpcc7942_0530 at 514.527 kb on + strand, within Synpcc7942_0530 at 514.540 kb on + strand, within Synpcc7942_0530 at 514.552 kb on - strand, within Synpcc7942_0530 at 514.559 kb on + strand, within Synpcc7942_0530 at 514.561 kb on + strand, within Synpcc7942_0530 at 514.562 kb on - strand, within Synpcc7942_0530 at 514.567 kb on - strand, within Synpcc7942_0530 at 514.579 kb on + strand, within Synpcc7942_0530 at 514.587 kb on - strand, within Synpcc7942_0530 at 514.618 kb on + strand, within Synpcc7942_0530 at 514.655 kb on - strand, within Synpcc7942_0530 at 514.670 kb on + strand, within Synpcc7942_0530 at 514.679 kb on + strand, within Synpcc7942_0530 at 514.715 kb on + strand, within Synpcc7942_0530 at 514.718 kb on + strand, within Synpcc7942_0530 at 514.736 kb on - strand, within Synpcc7942_0530 at 514.746 kb on - strand, within Synpcc7942_0530 at 514.764 kb on + strand, within Synpcc7942_0530 at 514.764 kb on + strand, within Synpcc7942_0530 at 514.807 kb on + strand, within Synpcc7942_0530 at 514.845 kb on + strand, within Synpcc7942_0530 at 514.853 kb on - strand, within Synpcc7942_0530 at 514.876 kb on - strand, within Synpcc7942_0530 at 514.886 kb on - strand, within Synpcc7942_0530 at 514.903 kb on + strand, within Synpcc7942_0530 at 514.903 kb on + strand, within Synpcc7942_0530 at 514.903 kb on + strand, within Synpcc7942_0530 at 514.911 kb on - strand, within Synpcc7942_0530 at 514.920 kb on + strand, within Synpcc7942_0530 at 514.928 kb on - strand, within Synpcc7942_0530 at 514.937 kb on + strand, within Synpcc7942_0530 at 514.937 kb on + strand, within Synpcc7942_0530 at 514.937 kb on + strand, within Synpcc7942_0530 at 514.945 kb on - strand, within Synpcc7942_0530 at 514.945 kb on - strand, within Synpcc7942_0530 at 514.945 kb on - strand, within Synpcc7942_0530 at 514.966 kb on - strand, within Synpcc7942_0530 at 514.968 kb on + strand, within Synpcc7942_0530 at 514.980 kb on + strand, within Synpcc7942_0530 at 514.998 kb on + strand, within Synpcc7942_0530 at 515.025 kb on + strand, within Synpcc7942_0530 at 515.033 kb on - strand, within Synpcc7942_0530 at 515.056 kb on + strand, within Synpcc7942_0530 at 515.076 kb on + strand, within Synpcc7942_0530 at 515.135 kb on - strand, within Synpcc7942_0530 at 515.145 kb on - strand, within Synpcc7942_0530 at 515.145 kb on - strand, within Synpcc7942_0530 at 515.145 kb on - strand, within Synpcc7942_0530 at 515.150 kb on - strand, within Synpcc7942_0530 at 515.151 kb on + strand, within Synpcc7942_0530 at 515.161 kb on - strand at 515.163 kb on + strand, within Synpcc7942_0530 at 515.174 kb on + strand, within Synpcc7942_0530 at 515.190 kb on + strand, within Synpcc7942_0530 at 515.197 kb on + strand at 515.197 kb on - strand at 515.212 kb on + strand at 515.217 kb on - strand at 515.245 kb on + strand at 515.268 kb on + strand at 515.318 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 8:16 remove 512,563 + -0.9 512,569 + -0.3 512,578 + +0.5 512,599 - -0.3 512,601 + +0.1 512,607 + -0.1 512,607 + +0.8 512,607 + +0.2 512,609 - -0.4 512,609 - -0.1 512,610 - +0.3 512,610 - +1.0 512,610 - -0.2 512,626 - -0.2 512,629 - +0.5 512,658 + +0.0 512,725 - Synpcc7942_0528 0.14 -0.2 512,757 + Synpcc7942_0528 0.17 -0.3 512,799 - Synpcc7942_0528 0.21 -0.3 512,812 + Synpcc7942_0528 0.23 -0.5 512,814 + Synpcc7942_0528 0.23 +0.5 512,879 - Synpcc7942_0528 0.29 -0.4 512,921 + Synpcc7942_0528 0.34 -0.1 512,943 + Synpcc7942_0528 0.36 +0.2 512,951 - Synpcc7942_0528 0.37 +0.2 512,957 - Synpcc7942_0528 0.37 +0.1 512,962 + Synpcc7942_0528 0.38 -0.1 512,970 - Synpcc7942_0528 0.38 -0.7 512,973 + Synpcc7942_0528 0.39 +0.5 512,983 + Synpcc7942_0528 0.40 -0.2 513,024 + Synpcc7942_0528 0.44 +0.2 513,029 - Synpcc7942_0528 0.44 -1.3 513,049 + Synpcc7942_0528 0.46 -0.6 513,160 - Synpcc7942_0528 0.58 -0.9 513,162 - Synpcc7942_0528 0.58 +0.2 513,171 - Synpcc7942_0528 0.59 -0.0 513,182 + Synpcc7942_0528 0.60 -0.3 513,203 + Synpcc7942_0528 0.62 -1.6 513,249 + Synpcc7942_0528 0.67 +0.4 513,251 + Synpcc7942_0528 0.67 -0.6 513,257 - Synpcc7942_0528 0.67 -0.8 513,262 - Synpcc7942_0528 0.68 -0.1 513,378 + Synpcc7942_0528 0.80 -0.9 513,386 - Synpcc7942_0528 0.81 -1.0 513,386 - Synpcc7942_0528 0.81 -0.0 513,386 - Synpcc7942_0528 0.81 -0.3 513,386 - Synpcc7942_0528 0.81 -0.1 513,416 + Synpcc7942_0528 0.84 -0.4 513,418 - Synpcc7942_0528 0.84 -1.3 513,447 + Synpcc7942_0528 0.87 -0.2 513,460 - Synpcc7942_0528 0.88 -0.2 513,476 - Synpcc7942_0528 0.90 -0.0 513,488 + -1.0 513,496 - +0.3 513,499 + +0.0 513,512 + +0.2 513,516 + -0.3 513,516 + +0.8 513,519 - +0.2 513,520 - +0.2 513,524 - -0.6 513,524 - +0.6 513,533 + -0.5 513,547 - -0.1 513,559 - +0.2 513,561 + -0.5 513,564 - +0.5 513,564 - +0.0 513,575 + -1.5 513,581 + -0.3 513,583 - -0.6 513,593 - -1.3 513,593 - -0.3 513,596 + +1.8 513,596 + -2.5 513,605 - -0.4 513,630 + nagB Synpcc7942_0529 0.11 -0.6 513,671 - nagB Synpcc7942_0529 0.16 -0.8 513,693 - nagB Synpcc7942_0529 0.19 -0.4 513,723 - nagB Synpcc7942_0529 0.23 -1.2 513,735 + nagB Synpcc7942_0529 0.24 -1.2 513,754 - nagB Synpcc7942_0529 0.27 -0.6 513,765 + nagB Synpcc7942_0529 0.28 -0.7 513,766 - nagB Synpcc7942_0529 0.28 -1.1 513,775 + nagB Synpcc7942_0529 0.29 -0.4 513,799 + nagB Synpcc7942_0529 0.32 -1.6 513,814 - nagB Synpcc7942_0529 0.34 -0.3 513,816 + nagB Synpcc7942_0529 0.35 -1.3 513,856 + nagB Synpcc7942_0529 0.40 -0.7 513,858 - nagB Synpcc7942_0529 0.40 -1.7 513,908 + nagB Synpcc7942_0529 0.46 -1.1 513,916 - nagB Synpcc7942_0529 0.47 -1.0 513,940 - nagB Synpcc7942_0529 0.50 -0.7 513,990 + nagB Synpcc7942_0529 0.57 +0.2 513,990 + nagB Synpcc7942_0529 0.57 -0.4 514,004 - nagB Synpcc7942_0529 0.58 -0.2 514,019 - nagB Synpcc7942_0529 0.60 -0.6 514,027 + nagB Synpcc7942_0529 0.61 -1.5 514,067 - nagB Synpcc7942_0529 0.66 -0.6 514,080 + nagB Synpcc7942_0529 0.68 -2.6 514,088 - nagB Synpcc7942_0529 0.69 -0.8 514,088 - nagB Synpcc7942_0529 0.69 -0.8 514,094 + nagB Synpcc7942_0529 0.70 -1.0 514,247 - nagB Synpcc7942_0529 0.89 -0.5 514,272 - +0.0 514,289 + -1.0 514,297 - -2.1 514,330 + +0.2 514,358 + -1.1 514,361 - +0.0 514,361 - -0.3 514,366 - -0.2 514,366 - +0.7 514,366 - -1.0 514,366 - +0.1 514,366 - +0.1 514,366 - -0.1 514,392 - -0.3 514,458 + Synpcc7942_0530 0.13 -0.0 514,458 + Synpcc7942_0530 0.13 +0.1 514,458 + Synpcc7942_0530 0.13 +0.2 514,458 + Synpcc7942_0530 0.13 +0.7 514,458 + Synpcc7942_0530 0.13 +0.6 514,466 - Synpcc7942_0530 0.14 +0.3 514,466 - Synpcc7942_0530 0.14 -0.2 514,466 - Synpcc7942_0530 0.14 +0.4 514,466 - Synpcc7942_0530 0.14 +0.2 514,466 - Synpcc7942_0530 0.14 +0.3 514,527 + Synpcc7942_0530 0.20 -0.3 514,540 + Synpcc7942_0530 0.21 -0.4 514,552 - Synpcc7942_0530 0.23 -0.1 514,559 + Synpcc7942_0530 0.23 -0.6 514,561 + Synpcc7942_0530 0.24 +0.3 514,562 - Synpcc7942_0530 0.24 -0.8 514,567 - Synpcc7942_0530 0.24 -0.3 514,579 + Synpcc7942_0530 0.26 -0.6 514,587 - Synpcc7942_0530 0.26 -1.1 514,618 + Synpcc7942_0530 0.30 -0.1 514,655 - Synpcc7942_0530 0.34 +0.2 514,670 + Synpcc7942_0530 0.35 -0.6 514,679 + Synpcc7942_0530 0.36 -0.1 514,715 + Synpcc7942_0530 0.40 -0.2 514,718 + Synpcc7942_0530 0.40 +0.4 514,736 - Synpcc7942_0530 0.42 +0.2 514,746 - Synpcc7942_0530 0.43 +0.0 514,764 + Synpcc7942_0530 0.45 -0.6 514,764 + Synpcc7942_0530 0.45 +1.2 514,807 + Synpcc7942_0530 0.49 -1.4 514,845 + Synpcc7942_0530 0.53 -0.2 514,853 - Synpcc7942_0530 0.54 +0.1 514,876 - Synpcc7942_0530 0.57 +0.3 514,886 - Synpcc7942_0530 0.58 -0.3 514,903 + Synpcc7942_0530 0.60 +0.0 514,903 + Synpcc7942_0530 0.60 -0.7 514,903 + Synpcc7942_0530 0.60 +0.2 514,911 - Synpcc7942_0530 0.60 -0.4 514,920 + Synpcc7942_0530 0.61 -0.5 514,928 - Synpcc7942_0530 0.62 +0.6 514,937 + Synpcc7942_0530 0.63 -0.3 514,937 + Synpcc7942_0530 0.63 -2.0 514,937 + Synpcc7942_0530 0.63 +0.1 514,945 - Synpcc7942_0530 0.64 -0.2 514,945 - Synpcc7942_0530 0.64 +0.1 514,945 - Synpcc7942_0530 0.64 -0.6 514,966 - Synpcc7942_0530 0.66 +0.4 514,968 + Synpcc7942_0530 0.66 -0.0 514,980 + Synpcc7942_0530 0.68 -1.5 514,998 + Synpcc7942_0530 0.69 +0.1 515,025 + Synpcc7942_0530 0.72 -0.6 515,033 - Synpcc7942_0530 0.73 -0.2 515,056 + Synpcc7942_0530 0.76 -0.1 515,076 + Synpcc7942_0530 0.78 -0.1 515,135 - Synpcc7942_0530 0.84 -0.9 515,145 - Synpcc7942_0530 0.85 +0.2 515,145 - Synpcc7942_0530 0.85 +0.6 515,145 - Synpcc7942_0530 0.85 -0.6 515,150 - Synpcc7942_0530 0.85 +0.2 515,151 + Synpcc7942_0530 0.86 -0.3 515,161 - +1.1 515,163 + Synpcc7942_0530 0.87 -0.1 515,174 + Synpcc7942_0530 0.88 +1.4 515,190 + Synpcc7942_0530 0.90 +0.0 515,197 + -0.7 515,197 - +0.2 515,212 + +0.3 515,217 - -0.3 515,245 + +0.2 515,268 + -0.9 515,318 - -0.2
Or see this region's nucleotide sequence