Experiment: D-Sorbitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5000 and Pf6N2E2_5001 are separated by 168 nucleotides Pf6N2E2_5001 and Pf6N2E2_5002 are separated by 81 nucleotides Pf6N2E2_5002 and Pf6N2E2_5003 overlap by 1 nucleotides
Pf6N2E2_5000 - Rare lipoprotein A precursor, at 5,766,197 to 5,767,222
_5000
Pf6N2E2_5001 - D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4), at 5,767,391 to 5,768,548
_5001
Pf6N2E2_5002 - Proposed lipoate regulatory protein YbeD, at 5,768,630 to 5,768,905
_5002
Pf6N2E2_5003 - Octanoate-[acyl-carrier-protein]-protein-N-octan oyltransferase, at 5,768,905 to 5,769,552
_5003
Position (kb)
5767
5768
5769 Strain fitness (log2 ratio)
-2
-1
0
1 at 5767.275 kb on + strand at 5767.275 kb on + strand at 5767.311 kb on + strand at 5767.311 kb on + strand at 5767.311 kb on + strand at 5767.312 kb on - strand at 5767.312 kb on - strand at 5767.602 kb on - strand, within Pf6N2E2_5001 at 5767.904 kb on + strand, within Pf6N2E2_5001 at 5768.231 kb on + strand, within Pf6N2E2_5001 at 5768.491 kb on + strand at 5768.531 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol (C) remove 5,767,275 + -0.2 5,767,275 + +0.6 5,767,311 + +0.6 5,767,311 + +0.4 5,767,311 + -0.1 5,767,312 - -0.2 5,767,312 - -1.9 5,767,602 - Pf6N2E2_5001 0.18 -0.5 5,767,904 + Pf6N2E2_5001 0.44 -1.2 5,768,231 + Pf6N2E2_5001 0.73 +0.9 5,768,491 + -0.7 5,768,531 + -1.1
Or see this region's nucleotide sequence