Experiment: D-Sorbitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1963 and Pf6N2E2_1964 are separated by 244 nucleotides Pf6N2E2_1964 and Pf6N2E2_1965 are separated by 54 nucleotides
Pf6N2E2_1963 - ABC transporter for D-sorbitol, periplasmic substrate-binding component (from data) , at 2,327,783 to 2,329,093
_1963
Pf6N2E2_1964 - Transcriptional regulator of various polyols utilization, AraC family, at 2,329,338 to 2,330,336
_1964
Pf6N2E2_1965 - Fructokinase (EC 2.7.1.4) (from data) , at 2,330,391 to 2,331,311
_1965
Position (kb)
2329
2330
2331 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2328.381 kb on - strand, within Pf6N2E2_1963 at 2328.410 kb on - strand, within Pf6N2E2_1963 at 2328.410 kb on - strand, within Pf6N2E2_1963 at 2328.418 kb on - strand, within Pf6N2E2_1963 at 2328.512 kb on - strand, within Pf6N2E2_1963 at 2328.675 kb on + strand, within Pf6N2E2_1963 at 2328.675 kb on + strand, within Pf6N2E2_1963 at 2328.676 kb on - strand, within Pf6N2E2_1963 at 2328.676 kb on - strand, within Pf6N2E2_1963 at 2328.697 kb on - strand, within Pf6N2E2_1963 at 2328.711 kb on + strand, within Pf6N2E2_1963 at 2328.711 kb on + strand, within Pf6N2E2_1963 at 2328.718 kb on + strand, within Pf6N2E2_1963 at 2328.812 kb on - strand, within Pf6N2E2_1963 at 2328.835 kb on - strand, within Pf6N2E2_1963 at 2328.835 kb on - strand, within Pf6N2E2_1963 at 2328.921 kb on - strand, within Pf6N2E2_1963 at 2329.063 kb on + strand at 2329.127 kb on + strand at 2329.128 kb on - strand at 2329.128 kb on - strand at 2329.130 kb on + strand at 2329.193 kb on + strand at 2329.194 kb on - strand at 2329.304 kb on + strand at 2329.304 kb on + strand at 2329.305 kb on - strand at 2329.338 kb on - strand at 2329.338 kb on - strand at 2329.364 kb on + strand at 2329.368 kb on + strand at 2329.370 kb on + strand at 2329.370 kb on + strand at 2329.371 kb on - strand at 2329.371 kb on - strand at 2329.371 kb on - strand at 2329.473 kb on + strand, within Pf6N2E2_1964 at 2329.524 kb on + strand, within Pf6N2E2_1964 at 2329.525 kb on - strand, within Pf6N2E2_1964 at 2329.647 kb on - strand, within Pf6N2E2_1964 at 2329.730 kb on - strand, within Pf6N2E2_1964 at 2329.738 kb on + strand, within Pf6N2E2_1964 at 2329.739 kb on - strand, within Pf6N2E2_1964 at 2329.756 kb on + strand, within Pf6N2E2_1964 at 2329.880 kb on - strand, within Pf6N2E2_1964 at 2329.974 kb on - strand, within Pf6N2E2_1964 at 2329.974 kb on - strand, within Pf6N2E2_1964 at 2329.974 kb on - strand, within Pf6N2E2_1964 at 2329.989 kb on + strand, within Pf6N2E2_1964 at 2329.989 kb on + strand, within Pf6N2E2_1964 at 2330.146 kb on + strand, within Pf6N2E2_1964 at 2330.191 kb on + strand, within Pf6N2E2_1964 at 2330.192 kb on - strand, within Pf6N2E2_1964 at 2330.267 kb on + strand at 2330.268 kb on - strand at 2330.365 kb on - strand at 2330.380 kb on + strand at 2330.381 kb on - strand at 2330.396 kb on + strand at 2330.396 kb on + strand at 2330.397 kb on - strand at 2330.397 kb on - strand at 2330.648 kb on - strand, within Pf6N2E2_1965 at 2330.651 kb on + strand, within Pf6N2E2_1965 at 2330.652 kb on - strand, within Pf6N2E2_1965 at 2330.652 kb on - strand, within Pf6N2E2_1965 at 2330.680 kb on - strand, within Pf6N2E2_1965 at 2330.704 kb on + strand, within Pf6N2E2_1965 at 2330.704 kb on + strand, within Pf6N2E2_1965 at 2330.771 kb on + strand, within Pf6N2E2_1965 at 2330.853 kb on + strand, within Pf6N2E2_1965 at 2330.853 kb on + strand, within Pf6N2E2_1965 at 2330.854 kb on - strand, within Pf6N2E2_1965 at 2330.854 kb on - strand, within Pf6N2E2_1965 at 2330.854 kb on - strand, within Pf6N2E2_1965 at 2330.854 kb on - strand, within Pf6N2E2_1965 at 2330.965 kb on + strand, within Pf6N2E2_1965 at 2330.965 kb on + strand, within Pf6N2E2_1965 at 2330.966 kb on - strand, within Pf6N2E2_1965 at 2330.966 kb on - strand, within Pf6N2E2_1965 at 2330.967 kb on + strand, within Pf6N2E2_1965 at 2330.967 kb on + strand, within Pf6N2E2_1965 at 2331.057 kb on - strand, within Pf6N2E2_1965 at 2331.066 kb on + strand, within Pf6N2E2_1965 at 2331.066 kb on + strand, within Pf6N2E2_1965 at 2331.066 kb on + strand, within Pf6N2E2_1965 at 2331.067 kb on - strand, within Pf6N2E2_1965 at 2331.173 kb on - strand, within Pf6N2E2_1965
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol (C) remove 2,328,381 - Pf6N2E2_1963 0.46 -3.9 2,328,410 - Pf6N2E2_1963 0.48 -2.3 2,328,410 - Pf6N2E2_1963 0.48 -3.1 2,328,418 - Pf6N2E2_1963 0.48 -1.5 2,328,512 - Pf6N2E2_1963 0.56 -2.8 2,328,675 + Pf6N2E2_1963 0.68 -2.7 2,328,675 + Pf6N2E2_1963 0.68 -2.3 2,328,676 - Pf6N2E2_1963 0.68 -3.8 2,328,676 - Pf6N2E2_1963 0.68 -4.3 2,328,697 - Pf6N2E2_1963 0.70 -3.3 2,328,711 + Pf6N2E2_1963 0.71 -3.9 2,328,711 + Pf6N2E2_1963 0.71 -3.7 2,328,718 + Pf6N2E2_1963 0.71 -4.3 2,328,812 - Pf6N2E2_1963 0.78 -0.2 2,328,835 - Pf6N2E2_1963 0.80 -3.5 2,328,835 - Pf6N2E2_1963 0.80 -4.7 2,328,921 - Pf6N2E2_1963 0.87 -4.1 2,329,063 + -2.5 2,329,127 + -4.7 2,329,128 - -4.6 2,329,128 - -0.8 2,329,130 + +0.5 2,329,193 + -2.6 2,329,194 - -2.5 2,329,304 + -1.5 2,329,304 + -0.5 2,329,305 - -0.4 2,329,338 - +0.9 2,329,338 - -2.1 2,329,364 + +1.2 2,329,368 + +0.1 2,329,370 + +1.4 2,329,370 + -2.7 2,329,371 - -0.1 2,329,371 - +0.3 2,329,371 - -0.4 2,329,473 + Pf6N2E2_1964 0.14 -0.2 2,329,524 + Pf6N2E2_1964 0.19 -0.4 2,329,525 - Pf6N2E2_1964 0.19 -0.6 2,329,647 - Pf6N2E2_1964 0.31 +0.5 2,329,730 - Pf6N2E2_1964 0.39 +0.9 2,329,738 + Pf6N2E2_1964 0.40 +0.0 2,329,739 - Pf6N2E2_1964 0.40 +0.3 2,329,756 + Pf6N2E2_1964 0.42 -0.8 2,329,880 - Pf6N2E2_1964 0.54 -0.8 2,329,974 - Pf6N2E2_1964 0.64 -3.8 2,329,974 - Pf6N2E2_1964 0.64 -2.7 2,329,974 - Pf6N2E2_1964 0.64 -1.9 2,329,989 + Pf6N2E2_1964 0.65 -2.1 2,329,989 + Pf6N2E2_1964 0.65 -2.2 2,330,146 + Pf6N2E2_1964 0.81 -2.1 2,330,191 + Pf6N2E2_1964 0.85 -0.5 2,330,192 - Pf6N2E2_1964 0.85 -1.7 2,330,267 + +0.4 2,330,268 - -3.3 2,330,365 - -2.8 2,330,380 + -0.2 2,330,381 - -1.5 2,330,396 + -1.2 2,330,396 + -2.7 2,330,397 - -3.9 2,330,397 - -1.5 2,330,648 - Pf6N2E2_1965 0.28 -3.8 2,330,651 + Pf6N2E2_1965 0.28 -2.7 2,330,652 - Pf6N2E2_1965 0.28 -4.1 2,330,652 - Pf6N2E2_1965 0.28 -2.5 2,330,680 - Pf6N2E2_1965 0.31 -3.1 2,330,704 + Pf6N2E2_1965 0.34 -1.6 2,330,704 + Pf6N2E2_1965 0.34 -2.9 2,330,771 + Pf6N2E2_1965 0.41 -1.8 2,330,853 + Pf6N2E2_1965 0.50 -1.1 2,330,853 + Pf6N2E2_1965 0.50 -2.1 2,330,854 - Pf6N2E2_1965 0.50 -1.7 2,330,854 - Pf6N2E2_1965 0.50 -2.9 2,330,854 - Pf6N2E2_1965 0.50 -2.4 2,330,854 - Pf6N2E2_1965 0.50 -2.7 2,330,965 + Pf6N2E2_1965 0.62 -3.2 2,330,965 + Pf6N2E2_1965 0.62 -3.1 2,330,966 - Pf6N2E2_1965 0.62 -0.5 2,330,966 - Pf6N2E2_1965 0.62 -1.8 2,330,967 + Pf6N2E2_1965 0.63 -2.3 2,330,967 + Pf6N2E2_1965 0.63 -2.7 2,331,057 - Pf6N2E2_1965 0.72 -3.2 2,331,066 + Pf6N2E2_1965 0.73 -3.8 2,331,066 + Pf6N2E2_1965 0.73 -4.2 2,331,066 + Pf6N2E2_1965 0.73 -3.4 2,331,067 - Pf6N2E2_1965 0.73 -3.2 2,331,173 - Pf6N2E2_1965 0.85 -3.6
Or see this region's nucleotide sequence