Experiment: D-Sorbitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1961 and Pf6N2E2_1962 are separated by 10 nucleotides Pf6N2E2_1962 and Pf6N2E2_1963 are separated by 143 nucleotides Pf6N2E2_1963 and Pf6N2E2_1964 are separated by 244 nucleotides
Pf6N2E2_1961 - ABC transporter for D-sorbitol, permease component 2 (from data) , at 2,325,953 to 2,326,786
_1961
Pf6N2E2_1962 - ABC transporter for D-sorbitol, permease component 1 (from data) , at 2,326,797 to 2,327,639
_1962
Pf6N2E2_1963 - ABC transporter for D-sorbitol, periplasmic substrate-binding component (from data) , at 2,327,783 to 2,329,093
_1963
Pf6N2E2_1964 - Transcriptional regulator of various polyols utilization, AraC family, at 2,329,338 to 2,330,336
_1964
Position (kb)
2327
2328
2329
2330 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2326.927 kb on + strand, within Pf6N2E2_1962 at 2327.337 kb on - strand, within Pf6N2E2_1962 at 2327.708 kb on + strand at 2327.727 kb on + strand at 2327.732 kb on - strand at 2327.773 kb on - strand at 2327.784 kb on + strand at 2327.785 kb on - strand at 2327.897 kb on + strand at 2327.937 kb on + strand, within Pf6N2E2_1963 at 2327.938 kb on - strand, within Pf6N2E2_1963 at 2327.953 kb on - strand, within Pf6N2E2_1963 at 2328.011 kb on + strand, within Pf6N2E2_1963 at 2328.040 kb on - strand, within Pf6N2E2_1963 at 2328.051 kb on + strand, within Pf6N2E2_1963 at 2328.104 kb on - strand, within Pf6N2E2_1963 at 2328.107 kb on + strand, within Pf6N2E2_1963 at 2328.108 kb on - strand, within Pf6N2E2_1963 at 2328.108 kb on - strand, within Pf6N2E2_1963 at 2328.108 kb on - strand, within Pf6N2E2_1963 at 2328.108 kb on - strand, within Pf6N2E2_1963 at 2328.108 kb on - strand, within Pf6N2E2_1963 at 2328.117 kb on + strand, within Pf6N2E2_1963 at 2328.118 kb on - strand, within Pf6N2E2_1963 at 2328.201 kb on + strand, within Pf6N2E2_1963 at 2328.202 kb on - strand, within Pf6N2E2_1963 at 2328.254 kb on + strand, within Pf6N2E2_1963 at 2328.381 kb on - strand, within Pf6N2E2_1963 at 2328.410 kb on - strand, within Pf6N2E2_1963 at 2328.410 kb on - strand, within Pf6N2E2_1963 at 2328.418 kb on - strand, within Pf6N2E2_1963 at 2328.512 kb on - strand, within Pf6N2E2_1963 at 2328.675 kb on + strand, within Pf6N2E2_1963 at 2328.675 kb on + strand, within Pf6N2E2_1963 at 2328.676 kb on - strand, within Pf6N2E2_1963 at 2328.676 kb on - strand, within Pf6N2E2_1963 at 2328.697 kb on - strand, within Pf6N2E2_1963 at 2328.711 kb on + strand, within Pf6N2E2_1963 at 2328.711 kb on + strand, within Pf6N2E2_1963 at 2328.718 kb on + strand, within Pf6N2E2_1963 at 2328.812 kb on - strand, within Pf6N2E2_1963 at 2328.835 kb on - strand, within Pf6N2E2_1963 at 2328.835 kb on - strand, within Pf6N2E2_1963 at 2328.921 kb on - strand, within Pf6N2E2_1963 at 2329.063 kb on + strand at 2329.127 kb on + strand at 2329.128 kb on - strand at 2329.128 kb on - strand at 2329.130 kb on + strand at 2329.193 kb on + strand at 2329.194 kb on - strand at 2329.304 kb on + strand at 2329.304 kb on + strand at 2329.305 kb on - strand at 2329.338 kb on - strand at 2329.338 kb on - strand at 2329.364 kb on + strand at 2329.368 kb on + strand at 2329.370 kb on + strand at 2329.370 kb on + strand at 2329.371 kb on - strand at 2329.371 kb on - strand at 2329.371 kb on - strand at 2329.473 kb on + strand, within Pf6N2E2_1964 at 2329.524 kb on + strand, within Pf6N2E2_1964 at 2329.525 kb on - strand, within Pf6N2E2_1964 at 2329.647 kb on - strand, within Pf6N2E2_1964 at 2329.730 kb on - strand, within Pf6N2E2_1964 at 2329.738 kb on + strand, within Pf6N2E2_1964 at 2329.739 kb on - strand, within Pf6N2E2_1964 at 2329.756 kb on + strand, within Pf6N2E2_1964 at 2329.880 kb on - strand, within Pf6N2E2_1964 at 2329.974 kb on - strand, within Pf6N2E2_1964 at 2329.974 kb on - strand, within Pf6N2E2_1964 at 2329.974 kb on - strand, within Pf6N2E2_1964 at 2329.989 kb on + strand, within Pf6N2E2_1964 at 2329.989 kb on + strand, within Pf6N2E2_1964
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol (C) remove 2,326,927 + Pf6N2E2_1962 0.15 -4.3 2,327,337 - Pf6N2E2_1962 0.64 -2.5 2,327,708 + -4.7 2,327,727 + -3.9 2,327,732 - -2.3 2,327,773 - -3.3 2,327,784 + -3.5 2,327,785 - -2.9 2,327,897 + -2.1 2,327,937 + Pf6N2E2_1963 0.12 -3.9 2,327,938 - Pf6N2E2_1963 0.12 -2.4 2,327,953 - Pf6N2E2_1963 0.13 -4.6 2,328,011 + Pf6N2E2_1963 0.17 -3.2 2,328,040 - Pf6N2E2_1963 0.20 -3.7 2,328,051 + Pf6N2E2_1963 0.20 -3.7 2,328,104 - Pf6N2E2_1963 0.24 -3.3 2,328,107 + Pf6N2E2_1963 0.25 -2.7 2,328,108 - Pf6N2E2_1963 0.25 -1.8 2,328,108 - Pf6N2E2_1963 0.25 -1.7 2,328,108 - Pf6N2E2_1963 0.25 -3.7 2,328,108 - Pf6N2E2_1963 0.25 -3.6 2,328,108 - Pf6N2E2_1963 0.25 -2.9 2,328,117 + Pf6N2E2_1963 0.25 -3.4 2,328,118 - Pf6N2E2_1963 0.26 -3.5 2,328,201 + Pf6N2E2_1963 0.32 -2.1 2,328,202 - Pf6N2E2_1963 0.32 -4.2 2,328,254 + Pf6N2E2_1963 0.36 -4.1 2,328,381 - Pf6N2E2_1963 0.46 -3.9 2,328,410 - Pf6N2E2_1963 0.48 -2.3 2,328,410 - Pf6N2E2_1963 0.48 -3.1 2,328,418 - Pf6N2E2_1963 0.48 -1.5 2,328,512 - Pf6N2E2_1963 0.56 -2.8 2,328,675 + Pf6N2E2_1963 0.68 -2.7 2,328,675 + Pf6N2E2_1963 0.68 -2.3 2,328,676 - Pf6N2E2_1963 0.68 -3.8 2,328,676 - Pf6N2E2_1963 0.68 -4.3 2,328,697 - Pf6N2E2_1963 0.70 -3.3 2,328,711 + Pf6N2E2_1963 0.71 -3.9 2,328,711 + Pf6N2E2_1963 0.71 -3.7 2,328,718 + Pf6N2E2_1963 0.71 -4.3 2,328,812 - Pf6N2E2_1963 0.78 -0.2 2,328,835 - Pf6N2E2_1963 0.80 -3.5 2,328,835 - Pf6N2E2_1963 0.80 -4.7 2,328,921 - Pf6N2E2_1963 0.87 -4.1 2,329,063 + -2.5 2,329,127 + -4.7 2,329,128 - -4.6 2,329,128 - -0.8 2,329,130 + +0.5 2,329,193 + -2.6 2,329,194 - -2.5 2,329,304 + -1.5 2,329,304 + -0.5 2,329,305 - -0.4 2,329,338 - +0.9 2,329,338 - -2.1 2,329,364 + +1.2 2,329,368 + +0.1 2,329,370 + +1.4 2,329,370 + -2.7 2,329,371 - -0.1 2,329,371 - +0.3 2,329,371 - -0.4 2,329,473 + Pf6N2E2_1964 0.14 -0.2 2,329,524 + Pf6N2E2_1964 0.19 -0.4 2,329,525 - Pf6N2E2_1964 0.19 -0.6 2,329,647 - Pf6N2E2_1964 0.31 +0.5 2,329,730 - Pf6N2E2_1964 0.39 +0.9 2,329,738 + Pf6N2E2_1964 0.40 +0.0 2,329,739 - Pf6N2E2_1964 0.40 +0.3 2,329,756 + Pf6N2E2_1964 0.42 -0.8 2,329,880 - Pf6N2E2_1964 0.54 -0.8 2,329,974 - Pf6N2E2_1964 0.64 -3.8 2,329,974 - Pf6N2E2_1964 0.64 -2.7 2,329,974 - Pf6N2E2_1964 0.64 -1.9 2,329,989 + Pf6N2E2_1964 0.65 -2.1 2,329,989 + Pf6N2E2_1964 0.65 -2.2
Or see this region's nucleotide sequence