Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1960

Experiment: D-Sorbitol (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1959 and Pf6N2E2_1960 are separated by 57 nucleotidesPf6N2E2_1960 and Pf6N2E2_1961 are separated by 57 nucleotidesPf6N2E2_1961 and Pf6N2E2_1962 are separated by 10 nucleotides Pf6N2E2_1959 - D-sorbitol dehydrogenase (EC 1.1.1.14) (from data), at 2,323,967 to 2,324,740 _1959 Pf6N2E2_1960 - ABC transporter for D-sorbitol, ATPase component (from data), at 2,324,798 to 2,325,895 _1960 Pf6N2E2_1961 - ABC transporter for D-sorbitol, permease component 2 (from data), at 2,325,953 to 2,326,786 _1961 Pf6N2E2_1962 - ABC transporter for D-sorbitol, permease component 1 (from data), at 2,326,797 to 2,327,639 _1962 Position (kb) 2324 2325 2326Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2323.800 kb on + strandat 2323.804 kb on + strandat 2323.805 kb on - strandat 2323.805 kb on - strandat 2323.805 kb on - strandat 2323.824 kb on + strandat 2323.999 kb on - strandat 2324.016 kb on + strandat 2324.123 kb on + strand, within Pf6N2E2_1959at 2324.251 kb on + strand, within Pf6N2E2_1959at 2324.578 kb on - strand, within Pf6N2E2_1959at 2324.637 kb on - strand, within Pf6N2E2_1959at 2324.664 kb on + strandat 2324.664 kb on + strandat 2324.664 kb on + strandat 2324.665 kb on - strandat 2324.665 kb on - strandat 2324.665 kb on - strandat 2324.665 kb on - strandat 2324.722 kb on + strandat 2324.748 kb on - strandat 2324.881 kb on - strandat 2324.881 kb on - strandat 2325.232 kb on - strand, within Pf6N2E2_1960at 2325.232 kb on - strand, within Pf6N2E2_1960at 2325.330 kb on + strand, within Pf6N2E2_1960at 2325.331 kb on - strand, within Pf6N2E2_1960at 2325.331 kb on - strand, within Pf6N2E2_1960at 2325.355 kb on + strand, within Pf6N2E2_1960at 2325.387 kb on - strand, within Pf6N2E2_1960at 2325.502 kb on + strand, within Pf6N2E2_1960at 2325.502 kb on + strand, within Pf6N2E2_1960at 2325.503 kb on - strand, within Pf6N2E2_1960at 2325.645 kb on + strand, within Pf6N2E2_1960at 2325.645 kb on + strand, within Pf6N2E2_1960at 2325.645 kb on + strand, within Pf6N2E2_1960at 2325.646 kb on - strand, within Pf6N2E2_1960at 2325.646 kb on - strand, within Pf6N2E2_1960at 2325.646 kb on - strand, within Pf6N2E2_1960at 2325.900 kb on - strandat 2326.021 kb on + strandat 2326.021 kb on + strandat 2326.021 kb on + strandat 2326.022 kb on - strandat 2326.022 kb on - strandat 2326.022 kb on - strandat 2326.299 kb on + strand, within Pf6N2E2_1961at 2326.306 kb on - strand, within Pf6N2E2_1961at 2326.345 kb on - strand, within Pf6N2E2_1961at 2326.345 kb on - strand, within Pf6N2E2_1961at 2326.345 kb on - strand, within Pf6N2E2_1961at 2326.401 kb on + strand, within Pf6N2E2_1961at 2326.401 kb on + strand, within Pf6N2E2_1961at 2326.401 kb on + strand, within Pf6N2E2_1961at 2326.401 kb on + strand, within Pf6N2E2_1961at 2326.401 kb on + strand, within Pf6N2E2_1961at 2326.401 kb on + strand, within Pf6N2E2_1961at 2326.405 kb on + strand, within Pf6N2E2_1961at 2326.406 kb on - strand, within Pf6N2E2_1961at 2326.406 kb on - strand, within Pf6N2E2_1961at 2326.581 kb on + strand, within Pf6N2E2_1961at 2326.582 kb on - strand, within Pf6N2E2_1961at 2326.608 kb on + strand, within Pf6N2E2_1961at 2326.609 kb on - strand, within Pf6N2E2_1961at 2326.622 kb on - strand, within Pf6N2E2_1961at 2326.748 kb on + strandat 2326.774 kb on + strandat 2326.774 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Sorbitol (C)
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2,323,800 + -0.8
2,323,804 + +0.6
2,323,805 - +0.7
2,323,805 - -1.3
2,323,805 - +0.3
2,323,824 + +0.8
2,323,999 - -3.4
2,324,016 + -2.5
2,324,123 + Pf6N2E2_1959 0.20 -3.4
2,324,251 + Pf6N2E2_1959 0.37 -4.6
2,324,578 - Pf6N2E2_1959 0.79 -4.1
2,324,637 - Pf6N2E2_1959 0.87 -3.4
2,324,664 + -3.7
2,324,664 + -4.1
2,324,664 + -2.8
2,324,665 - -4.1
2,324,665 - -4.8
2,324,665 - -4.1
2,324,665 - -0.5
2,324,722 + -4.0
2,324,748 - -1.0
2,324,881 - -3.7
2,324,881 - -3.3
2,325,232 - Pf6N2E2_1960 0.40 -1.8
2,325,232 - Pf6N2E2_1960 0.40 -2.1
2,325,330 + Pf6N2E2_1960 0.48 +0.0
2,325,331 - Pf6N2E2_1960 0.49 -4.1
2,325,331 - Pf6N2E2_1960 0.49 -2.7
2,325,355 + Pf6N2E2_1960 0.51 -3.7
2,325,387 - Pf6N2E2_1960 0.54 -2.4
2,325,502 + Pf6N2E2_1960 0.64 -2.5
2,325,502 + Pf6N2E2_1960 0.64 -1.2
2,325,503 - Pf6N2E2_1960 0.64 -4.3
2,325,645 + Pf6N2E2_1960 0.77 -3.8
2,325,645 + Pf6N2E2_1960 0.77 -3.8
2,325,645 + Pf6N2E2_1960 0.77 -4.0
2,325,646 - Pf6N2E2_1960 0.77 -4.4
2,325,646 - Pf6N2E2_1960 0.77 -3.1
2,325,646 - Pf6N2E2_1960 0.77 -4.1
2,325,900 - -2.9
2,326,021 + -1.5
2,326,021 + -4.8
2,326,021 + -3.6
2,326,022 - -0.9
2,326,022 - -3.7
2,326,022 - -3.9
2,326,299 + Pf6N2E2_1961 0.41 -3.1
2,326,306 - Pf6N2E2_1961 0.42 -4.1
2,326,345 - Pf6N2E2_1961 0.47 -4.7
2,326,345 - Pf6N2E2_1961 0.47 -3.1
2,326,345 - Pf6N2E2_1961 0.47 -3.6
2,326,401 + Pf6N2E2_1961 0.54 -4.7
2,326,401 + Pf6N2E2_1961 0.54 -3.6
2,326,401 + Pf6N2E2_1961 0.54 -2.8
2,326,401 + Pf6N2E2_1961 0.54 -2.1
2,326,401 + Pf6N2E2_1961 0.54 -3.9
2,326,401 + Pf6N2E2_1961 0.54 -2.3
2,326,405 + Pf6N2E2_1961 0.54 -1.8
2,326,406 - Pf6N2E2_1961 0.54 -3.5
2,326,406 - Pf6N2E2_1961 0.54 -3.9
2,326,581 + Pf6N2E2_1961 0.75 -2.5
2,326,582 - Pf6N2E2_1961 0.75 -3.8
2,326,608 + Pf6N2E2_1961 0.79 -2.9
2,326,609 - Pf6N2E2_1961 0.79 -3.4
2,326,622 - Pf6N2E2_1961 0.80 -2.6
2,326,748 + -4.1
2,326,774 + -4.4
2,326,774 + -3.9

Or see this region's nucleotide sequence