Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1651

Experiment: D-Sorbitol (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1650 and Pf6N2E2_1651 are separated by 84 nucleotidesPf6N2E2_1651 and Pf6N2E2_1652 are separated by 48 nucleotidesPf6N2E2_1652 and Pf6N2E2_1653 are separated by 169 nucleotides Pf6N2E2_1650 - Sucrose alpha-glucosidase (EC 3.2.1.48) (from data), at 1,955,842 to 1,957,344 _1650 Pf6N2E2_1651 - Sucrose specific transcriptional regulator CscR, LacI family, at 1,957,429 to 1,958,412 _1651 Pf6N2E2_1652 - Transcriptional regulator, TetR family, at 1,958,461 to 1,959,048 _1652 Pf6N2E2_1653 - NADH:flavin oxidoreductase/NADH oxidase, at 1,959,218 to 1,960,312 _1653 Position (kb) 1957 1958 1959Strain fitness (log2 ratio) -3 -2 -1 0 1at 1956.508 kb on + strand, within Pf6N2E2_1650at 1956.508 kb on + strand, within Pf6N2E2_1650at 1956.509 kb on - strand, within Pf6N2E2_1650at 1956.563 kb on - strand, within Pf6N2E2_1650at 1956.563 kb on - strand, within Pf6N2E2_1650at 1956.592 kb on + strand, within Pf6N2E2_1650at 1956.610 kb on + strand, within Pf6N2E2_1650at 1956.611 kb on - strand, within Pf6N2E2_1650at 1957.060 kb on + strand, within Pf6N2E2_1650at 1957.061 kb on - strand, within Pf6N2E2_1650at 1957.063 kb on + strand, within Pf6N2E2_1650at 1957.176 kb on - strand, within Pf6N2E2_1650at 1957.243 kb on + strandat 1957.275 kb on - strandat 1958.410 kb on + strandat 1958.410 kb on + strandat 1958.410 kb on + strandat 1958.411 kb on - strandat 1958.439 kb on + strandat 1958.440 kb on - strandat 1958.462 kb on + strandat 1958.462 kb on + strandat 1958.796 kb on - strand, within Pf6N2E2_1652at 1958.796 kb on - strand, within Pf6N2E2_1652at 1958.796 kb on - strand, within Pf6N2E2_1652at 1958.816 kb on - strand, within Pf6N2E2_1652at 1959.245 kb on + strandat 1959.245 kb on + strandat 1959.246 kb on - strandat 1959.302 kb on - strandat 1959.347 kb on + strand, within Pf6N2E2_1653at 1959.348 kb on - strand, within Pf6N2E2_1653at 1959.350 kb on + strand, within Pf6N2E2_1653at 1959.351 kb on - strand, within Pf6N2E2_1653

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Sorbitol (C)
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1,956,508 + Pf6N2E2_1650 0.44 +0.4
1,956,508 + Pf6N2E2_1650 0.44 +1.3
1,956,509 - Pf6N2E2_1650 0.44 -0.2
1,956,563 - Pf6N2E2_1650 0.48 -0.1
1,956,563 - Pf6N2E2_1650 0.48 -0.6
1,956,592 + Pf6N2E2_1650 0.50 -0.1
1,956,610 + Pf6N2E2_1650 0.51 -1.3
1,956,611 - Pf6N2E2_1650 0.51 -2.3
1,957,060 + Pf6N2E2_1650 0.81 +0.7
1,957,061 - Pf6N2E2_1650 0.81 -0.0
1,957,063 + Pf6N2E2_1650 0.81 -0.2
1,957,176 - Pf6N2E2_1650 0.89 -0.3
1,957,243 + +1.1
1,957,275 - -0.1
1,958,410 + +0.4
1,958,410 + -3.3
1,958,410 + +0.0
1,958,411 - -0.1
1,958,439 + +0.4
1,958,440 - -0.3
1,958,462 + +0.5
1,958,462 + +0.1
1,958,796 - Pf6N2E2_1652 0.57 -0.0
1,958,796 - Pf6N2E2_1652 0.57 +0.5
1,958,796 - Pf6N2E2_1652 0.57 +0.7
1,958,816 - Pf6N2E2_1652 0.60 +1.6
1,959,245 + +0.5
1,959,245 + +0.6
1,959,246 - +1.2
1,959,302 - -0.3
1,959,347 + Pf6N2E2_1653 0.12 +1.2
1,959,348 - Pf6N2E2_1653 0.12 +0.0
1,959,350 + Pf6N2E2_1653 0.12 -1.3
1,959,351 - Pf6N2E2_1653 0.12 +0.0

Or see this region's nucleotide sequence