Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00820 and MMP_RS00825 are separated by 27 nucleotides MMP_RS00825 and MMP_RS00830 are separated by 15 nucleotides MMP_RS00830 and MMP_RS00835 are separated by 31 nucleotides
MMP_RS00820: MMP_RS00820 - DUF2117 domain-containing protein, at 153,055 to 154,170
_RS00820
MMP_RS00825: MMP_RS00825 - fumarylacetoacetate hydrolase family protein, at 154,198 to 154,806
_RS00825
MMP_RS00830: MMP_RS00830 - purine phosphoribosyltransferase family protein, at 154,822 to 155,379
_RS00830
MMP_RS00835: MMP_RS00835 - FAD-dependent oxidoreductase, at 155,411 to 156,817
_RS00835
Position (kb) 154
155 Strain fitness (log2 ratio) -1
0
1 at 153.213 kb on - strand at 153.327 kb on - strand at 153.345 kb on - strand at 153.355 kb on - strand at 153.562 kb on + strand at 153.562 kb on + strand at 153.574 kb on + strand at 153.592 kb on - strand at 154.681 kb on + strand, within MMP_RS00825 at 154.684 kb on - strand, within MMP_RS00825 at 154.684 kb on - strand, within MMP_RS00825 at 154.711 kb on - strand, within MMP_RS00825 at 154.740 kb on - strand, within MMP_RS00825 at 154.798 kb on - strand at 155.145 kb on + strand, within MMP_RS00830 at 155.309 kb on + strand, within MMP_RS00830 at 155.443 kb on + strand at 155.616 kb on - strand at 155.638 kb on - strand at 155.663 kb on + strand at 155.777 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 153,213 - +0.2 153,327 - -0.6 153,345 - +0.3 153,355 - +0.9 153,562 + +0.8 153,562 + +1.1 153,574 + +0.2 153,592 - +0.5 154,681 + MMP_RS00825 0.79 -0.4 154,684 - MMP_RS00825 0.80 +0.4 154,684 - MMP_RS00825 0.80 +0.0 154,711 - MMP_RS00825 0.84 -0.5 154,740 - MMP_RS00825 0.89 -0.6 154,798 - +1.7 155,145 + MMP_RS00830 0.58 +0.0 155,309 + MMP_RS00830 0.87 +0.2 155,443 + +0.7 155,616 - +1.4 155,638 - +1.1 155,663 + +0.8 155,777 + +0.6
Or see this region's nucleotide sequence