Experiment: MoYLS4 with Polymyxin B sulfate 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_3126 and DvMF_3127 are separated by 360 nucleotides DvMF_3127 and DvMF_3128 overlap by 11 nucleotides DvMF_3128 and DvMF_3129 are separated by 29 nucleotides DvMF_3129 and DvMF_3130 are separated by 83 nucleotides
DvMF_3126: DvMF_3126 - inosine-5'-monophosphate dehydrogenase (RefSeq), at 3,946,094 to 3,947,554
_3126
DvMF_3127: DvMF_3127 - Tetratricopeptide TPR_2 repeat protein (RefSeq), at 3,947,915 to 3,948,535
_3127
DvMF_3128: DvMF_3128 - hypothetical protein (RefSeq), at 3,948,525 to 3,948,665
_3128
DvMF_3129: DvMF_3129 - cytochrome c assembly protein (RefSeq), at 3,948,695 to 3,949,381
_3129
DvMF_3130: DvMF_3130 - cytochrome c-type biogenesis protein CcmB (RefSeq), at 3,949,465 to 3,950,142
_3130
Position (kb)
3947
3948
3949 Strain fitness (log2 ratio)
-1
0
1 at 3947.774 kb on + strand at 3947.784 kb on + strand at 3947.922 kb on + strand at 3948.021 kb on + strand, within DvMF_3127 at 3948.029 kb on - strand, within DvMF_3127 at 3948.029 kb on - strand, within DvMF_3127 at 3948.132 kb on - strand, within DvMF_3127 at 3948.280 kb on - strand, within DvMF_3127 at 3948.285 kb on - strand, within DvMF_3127 at 3948.350 kb on + strand, within DvMF_3127 at 3948.377 kb on - strand, within DvMF_3127 at 3948.428 kb on + strand, within DvMF_3127 at 3948.460 kb on + strand, within DvMF_3127 at 3949.462 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.125 mg/ml remove 3,947,774 + -0.4 3,947,784 + -0.1 3,947,922 + -0.2 3,948,021 + DvMF_3127 0.17 +0.0 3,948,029 - DvMF_3127 0.18 -0.1 3,948,029 - DvMF_3127 0.18 -0.1 3,948,132 - DvMF_3127 0.35 -0.2 3,948,280 - DvMF_3127 0.59 -1.2 3,948,285 - DvMF_3127 0.60 -1.0 3,948,350 + DvMF_3127 0.70 -0.8 3,948,377 - DvMF_3127 0.74 +0.3 3,948,428 + DvMF_3127 0.83 -0.7 3,948,460 + DvMF_3127 0.88 -1.1 3,949,462 + -0.4
Or see this region's nucleotide sequence