Experiment: MoYLS4 with Polymyxin B sulfate 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2617 and DvMF_2618 are separated by 299 nucleotides DvMF_2618 and DvMF_2619 are separated by 24 nucleotides DvMF_2619 and DvMF_2620 are separated by 158 nucleotides
DvMF_2617: DvMF_2617 - nitrogenase molybdenum-iron protein beta chain (RefSeq), at 3,305,945 to 3,307,321
_2617
DvMF_2618: DvMF_2618 - Dinitrogenase iron-molybdenum cofactor biosynthesis protein (RefSeq), at 3,307,621 to 3,308,880
_2618
DvMF_2619: DvMF_2619 - ferredoxin, 2Fe-2S (RefSeq), at 3,308,905 to 3,309,207
_2619
DvMF_2620: DvMF_2620 - nitrogenase MoFe cofactor biosynthesis protein NifE (RefSeq), at 3,309,366 to 3,310,790
_2620
Position (kb)
3307
3308
3309 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3306.634 kb on - strand, within DvMF_2617 at 3306.634 kb on - strand, within DvMF_2617 at 3306.668 kb on - strand, within DvMF_2617 at 3306.729 kb on + strand, within DvMF_2617 at 3306.739 kb on + strand, within DvMF_2617 at 3306.858 kb on - strand, within DvMF_2617 at 3307.114 kb on + strand, within DvMF_2617 at 3307.228 kb on - strand at 3307.230 kb on - strand at 3307.574 kb on - strand at 3307.585 kb on - strand at 3307.828 kb on + strand, within DvMF_2618 at 3307.836 kb on - strand, within DvMF_2618 at 3307.836 kb on - strand, within DvMF_2618 at 3308.016 kb on + strand, within DvMF_2618 at 3308.031 kb on + strand, within DvMF_2618 at 3308.153 kb on - strand, within DvMF_2618 at 3308.717 kb on - strand, within DvMF_2618 at 3308.848 kb on + strand at 3308.964 kb on - strand, within DvMF_2619 at 3309.124 kb on + strand, within DvMF_2619 at 3309.302 kb on - strand at 3309.504 kb on - strand at 3309.549 kb on + strand, within DvMF_2620 at 3309.551 kb on + strand, within DvMF_2620 at 3309.571 kb on - strand, within DvMF_2620 at 3309.585 kb on - strand, within DvMF_2620 at 3309.606 kb on + strand, within DvMF_2620
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.125 mg/ml remove 3,306,634 - DvMF_2617 0.50 -0.7 3,306,634 - DvMF_2617 0.50 -0.5 3,306,668 - DvMF_2617 0.53 -0.3 3,306,729 + DvMF_2617 0.57 -0.3 3,306,739 + DvMF_2617 0.58 -0.5 3,306,858 - DvMF_2617 0.66 -0.4 3,307,114 + DvMF_2617 0.85 -0.0 3,307,228 - -0.4 3,307,230 - -0.1 3,307,574 - -0.6 3,307,585 - -0.9 3,307,828 + DvMF_2618 0.16 -0.5 3,307,836 - DvMF_2618 0.17 +0.1 3,307,836 - DvMF_2618 0.17 -1.1 3,308,016 + DvMF_2618 0.31 -0.9 3,308,031 + DvMF_2618 0.33 -0.7 3,308,153 - DvMF_2618 0.42 -0.8 3,308,717 - DvMF_2618 0.87 -0.4 3,308,848 + -0.4 3,308,964 - DvMF_2619 0.19 -0.5 3,309,124 + DvMF_2619 0.72 -0.4 3,309,302 - -0.2 3,309,504 - -0.7 3,309,549 + DvMF_2620 0.13 -3.7 3,309,551 + DvMF_2620 0.13 -0.5 3,309,571 - DvMF_2620 0.14 -0.6 3,309,585 - DvMF_2620 0.15 -0.4 3,309,606 + DvMF_2620 0.17 +0.1
Or see this region's nucleotide sequence