Experiment: MoYLS4 with Polymyxin B sulfate 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2295 and DvMF_2296 are separated by 256 nucleotides DvMF_2296 and DvMF_2297 are separated by 39 nucleotides DvMF_2297 and DvMF_2298 are separated by 386 nucleotides
DvMF_2295: DvMF_2295 - chaperone protein DnaJ (RefSeq), at 2,883,088 to 2,884,212
_2295
DvMF_2296: DvMF_2296 - hypothetical protein (RefSeq), at 2,884,469 to 2,885,122
_2296
DvMF_2297: DvMF_2297 - hypothetical protein (RefSeq), at 2,885,162 to 2,885,677
_2297
DvMF_2298: DvMF_2298 - outer membrane efflux protein (RefSeq), at 2,886,064 to 2,887,641
_2298
Position (kb)
2884
2885
2886 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2883.647 kb on + strand, within DvMF_2295 at 2883.855 kb on + strand, within DvMF_2295 at 2884.034 kb on + strand, within DvMF_2295 at 2884.088 kb on + strand, within DvMF_2295 at 2884.554 kb on + strand, within DvMF_2296 at 2884.640 kb on - strand, within DvMF_2296 at 2884.668 kb on - strand, within DvMF_2296 at 2884.702 kb on + strand, within DvMF_2296 at 2884.735 kb on + strand, within DvMF_2296 at 2884.951 kb on - strand, within DvMF_2296 at 2885.067 kb on + strand at 2885.236 kb on - strand, within DvMF_2297 at 2885.340 kb on - strand, within DvMF_2297 at 2885.454 kb on - strand, within DvMF_2297 at 2885.488 kb on - strand, within DvMF_2297 at 2885.786 kb on - strand at 2885.914 kb on + strand at 2886.043 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.125 mg/ml remove 2,883,647 + DvMF_2295 0.50 +1.8 2,883,855 + DvMF_2295 0.68 +1.7 2,884,034 + DvMF_2295 0.84 +2.9 2,884,088 + DvMF_2295 0.89 +3.6 2,884,554 + DvMF_2296 0.13 -1.4 2,884,640 - DvMF_2296 0.26 -1.4 2,884,668 - DvMF_2296 0.30 -0.2 2,884,702 + DvMF_2296 0.36 -1.3 2,884,735 + DvMF_2296 0.41 -0.7 2,884,951 - DvMF_2296 0.74 -0.7 2,885,067 + -1.1 2,885,236 - DvMF_2297 0.14 -1.7 2,885,340 - DvMF_2297 0.34 -2.7 2,885,454 - DvMF_2297 0.57 -0.4 2,885,488 - DvMF_2297 0.63 -0.6 2,885,786 - -0.5 2,885,914 + -1.3 2,886,043 - -1.0
Or see this region's nucleotide sequence