Experiment: MoYLS4 with Polymyxin B sulfate 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2063 and DvMF_2064 are separated by 173 nucleotides DvMF_2064 and DvMF_2065 are separated by 227 nucleotides DvMF_2065 and DvMF_2066 are separated by 109 nucleotides
DvMF_2063: DvMF_2063 - protein of unknown function DUF214 (RefSeq), at 2,558,427 to 2,559,620
_2063
DvMF_2064: DvMF_2064 - hypothetical protein (RefSeq), at 2,559,794 to 2,560,255
_2064
DvMF_2065: DvMF_2065 - ybaK/ebsC protein (RefSeq), at 2,560,483 to 2,560,947
_2065
DvMF_2066: DvMF_2066 - heavy metal translocating P-type ATPase (RefSeq), at 2,561,057 to 2,563,573
_2066
Position (kb)
2559
2560
2561 Strain fitness (log2 ratio)
-2
-1
0
1 at 2558.841 kb on - strand, within DvMF_2063 at 2558.866 kb on + strand, within DvMF_2063 at 2558.866 kb on + strand, within DvMF_2063 at 2558.874 kb on - strand, within DvMF_2063 at 2558.959 kb on - strand, within DvMF_2063 at 2559.210 kb on + strand, within DvMF_2063 at 2559.270 kb on - strand, within DvMF_2063 at 2559.614 kb on - strand at 2559.924 kb on + strand, within DvMF_2064 at 2559.983 kb on - strand, within DvMF_2064 at 2560.352 kb on - strand at 2560.401 kb on - strand at 2560.512 kb on + strand at 2560.608 kb on - strand, within DvMF_2065 at 2560.809 kb on + strand, within DvMF_2065 at 2560.898 kb on - strand, within DvMF_2065 at 2561.054 kb on + strand at 2561.142 kb on - strand at 2561.142 kb on - strand at 2561.172 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.125 mg/ml remove 2,558,841 - DvMF_2063 0.35 -0.0 2,558,866 + DvMF_2063 0.37 -0.7 2,558,866 + DvMF_2063 0.37 +0.3 2,558,874 - DvMF_2063 0.37 -0.5 2,558,959 - DvMF_2063 0.45 -0.4 2,559,210 + DvMF_2063 0.66 -1.2 2,559,270 - DvMF_2063 0.71 -0.5 2,559,614 - -0.4 2,559,924 + DvMF_2064 0.28 -0.7 2,559,983 - DvMF_2064 0.41 -1.1 2,560,352 - -0.1 2,560,401 - +0.2 2,560,512 + -0.9 2,560,608 - DvMF_2065 0.27 -0.7 2,560,809 + DvMF_2065 0.70 +0.4 2,560,898 - DvMF_2065 0.89 -0.5 2,561,054 + -2.7 2,561,142 - +0.3 2,561,142 - -0.3 2,561,172 - -0.3
Or see this region's nucleotide sequence