Experiment: MoYLS4 with Polymyxin B sulfate 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0444 and DvMF_0445 are separated by 180 nucleotides DvMF_0445 and DvMF_0446 are separated by 6 nucleotides DvMF_0446 and DvMF_0447 are separated by 299 nucleotides
DvMF_0444: DvMF_0444 - hypothetical protein (RefSeq), at 520,184 to 520,546
_0444
DvMF_0445: DvMF_0445 - 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq), at 520,727 to 521,233
_0445
DvMF_0446: DvMF_0446 - putative glutamate synthase subunit beta (RefSeq), at 521,240 to 522,331
_0446
DvMF_0447: DvMF_0447 - amino acid-binding ACT domain protein (RefSeq), at 522,631 to 523,062
_0447
Position (kb) 521
522
523 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 520.906 kb on + strand, within DvMF_0445 at 521.055 kb on - strand, within DvMF_0445 at 521.122 kb on - strand, within DvMF_0445 at 521.317 kb on - strand at 521.323 kb on + strand at 521.898 kb on + strand, within DvMF_0446 at 521.906 kb on - strand, within DvMF_0446 at 521.906 kb on - strand, within DvMF_0446 at 522.000 kb on - strand, within DvMF_0446 at 522.165 kb on - strand, within DvMF_0446 at 522.179 kb on - strand, within DvMF_0446 at 522.321 kb on + strand at 522.534 kb on - strand at 522.792 kb on + strand, within DvMF_0447 at 522.818 kb on - strand, within DvMF_0447
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.125 mg/ml remove 520,906 + DvMF_0445 0.35 -3.3 521,055 - DvMF_0445 0.65 -0.1 521,122 - DvMF_0445 0.78 -0.3 521,317 - -0.6 521,323 + -0.1 521,898 + DvMF_0446 0.60 +0.1 521,906 - DvMF_0446 0.61 +0.0 521,906 - DvMF_0446 0.61 -1.0 522,000 - DvMF_0446 0.70 -0.8 522,165 - DvMF_0446 0.85 -0.4 522,179 - DvMF_0446 0.86 -0.2 522,321 + -0.9 522,534 - -0.7 522,792 + DvMF_0447 0.37 -0.1 522,818 - DvMF_0447 0.43 +0.0
Or see this region's nucleotide sequence