Experiment: MoYLS4 with Polymyxin B sulfate 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0420 and DvMF_0421 are separated by 153 nucleotides DvMF_0421 and DvMF_0422 are separated by 129 nucleotides DvMF_0422 and DvMF_0423 are separated by 4 nucleotides DvMF_0423 and DvMF_0424 are separated by 247 nucleotides
DvMF_0420: DvMF_0420 - putative manganese-dependent inorganic pyrophosphatase (RefSeq), at 495,720 to 496,640
_0420
DvMF_0421: DvMF_0421 - hypothetical protein (RefSeq), at 496,794 to 497,363
_0421
DvMF_0422: DvMF_0422 - PTS system sorbose subfamily IIB component (RefSeq), at 497,493 to 497,957
_0422
DvMF_0423: DvMF_0423 - PTS system fructose subfamily IIA component (RefSeq), at 497,962 to 498,480
_0423
DvMF_0424: DvMF_0424 - hypothetical protein (RefSeq), at 498,728 to 499,609
_0424
Position (kb) 497
498 Strain fitness (log2 ratio) -2
-1
0
1 at 496.913 kb on - strand, within DvMF_0421 at 497.089 kb on - strand, within DvMF_0421 at 497.305 kb on + strand, within DvMF_0421 at 497.308 kb on - strand at 497.773 kb on - strand, within DvMF_0422 at 498.096 kb on + strand, within DvMF_0423 at 498.096 kb on + strand, within DvMF_0423 at 498.270 kb on + strand, within DvMF_0423 at 498.286 kb on + strand, within DvMF_0423 at 498.418 kb on - strand, within DvMF_0423 at 498.438 kb on - strand at 498.438 kb on - strand at 498.455 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.125 mg/ml remove 496,913 - DvMF_0421 0.21 +1.4 497,089 - DvMF_0421 0.52 +0.3 497,305 + DvMF_0421 0.90 -0.2 497,308 - +0.5 497,773 - DvMF_0422 0.60 +1.1 498,096 + DvMF_0423 0.26 -2.4 498,096 + DvMF_0423 0.26 -2.2 498,270 + DvMF_0423 0.59 -0.9 498,286 + DvMF_0423 0.62 -1.9 498,418 - DvMF_0423 0.88 -1.6 498,438 - -2.4 498,438 - -1.6 498,455 + -2.7
Or see this region's nucleotide sequence