Experiment: MoYLS4 with Polymyxin B sulfate 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0322 and DvMF_0323 overlap by 4 nucleotides DvMF_0323 and DvMF_0324 overlap by 4 nucleotides DvMF_0324 and DvMF_0325 overlap by 13 nucleotides
DvMF_0322: DvMF_0322 - heterodisulfide reductase, C subunit (RefSeq), at 375,516 to 376,076
_0322
DvMF_0323: DvMF_0323 - CoB--CoM heterodisulfide reductase (RefSeq), at 376,073 to 377,053
_0323
DvMF_0324: DvMF_0324 - 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq), at 377,050 to 379,008
_0324
DvMF_0325: DvMF_0325 - methyl-viologen-reducing hydrogenase delta subunit (RefSeq), at 378,996 to 380,501
_0325
Position (kb) 377
378
379
380 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 376.676 kb on - strand, within DvMF_0323 at 376.828 kb on - strand, within DvMF_0323 at 376.981 kb on + strand at 377.019 kb on - strand at 377.118 kb on + strand at 377.216 kb on + strand at 377.502 kb on + strand, within DvMF_0324 at 377.502 kb on + strand, within DvMF_0324 at 377.520 kb on - strand, within DvMF_0324 at 378.049 kb on - strand, within DvMF_0324 at 378.049 kb on - strand, within DvMF_0324 at 378.100 kb on - strand, within DvMF_0324 at 378.100 kb on - strand, within DvMF_0324 at 378.387 kb on - strand, within DvMF_0324 at 378.721 kb on - strand at 378.984 kb on + strand at 379.359 kb on + strand, within DvMF_0325 at 379.747 kb on + strand, within DvMF_0325 at 379.777 kb on + strand, within DvMF_0325
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.125 mg/ml remove 376,676 - DvMF_0323 0.61 +4.1 376,828 - DvMF_0323 0.77 +4.2 376,981 + +0.6 377,019 - +3.1 377,118 + +3.9 377,216 + +4.0 377,502 + DvMF_0324 0.23 +4.0 377,502 + DvMF_0324 0.23 +3.9 377,520 - DvMF_0324 0.24 +4.0 378,049 - DvMF_0324 0.51 +3.8 378,049 - DvMF_0324 0.51 +4.1 378,100 - DvMF_0324 0.54 +4.0 378,100 - DvMF_0324 0.54 +4.1 378,387 - DvMF_0324 0.68 +3.5 378,721 - +4.9 378,984 + +3.5 379,359 + DvMF_0325 0.24 +4.0 379,747 + DvMF_0325 0.50 +4.0 379,777 + DvMF_0325 0.52 +4.0
Or see this region's nucleotide sequence