Experiment: MoYLS4 with Polymyxin B sulfate 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0321 and DvMF_0322 are separated by 279 nucleotides DvMF_0322 and DvMF_0323 overlap by 4 nucleotides DvMF_0323 and DvMF_0324 overlap by 4 nucleotides
DvMF_0321: DvMF_0321 - iron-containing alcohol dehydrogenase (RefSeq), at 374,055 to 375,236
_0321
DvMF_0322: DvMF_0322 - heterodisulfide reductase, C subunit (RefSeq), at 375,516 to 376,076
_0322
DvMF_0323: DvMF_0323 - CoB--CoM heterodisulfide reductase (RefSeq), at 376,073 to 377,053
_0323
DvMF_0324: DvMF_0324 - 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq), at 377,050 to 379,008
_0324
Position (kb) 375
376
377 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 374.657 kb on - strand, within DvMF_0321 at 374.657 kb on - strand, within DvMF_0321 at 374.708 kb on - strand, within DvMF_0321 at 374.720 kb on + strand, within DvMF_0321 at 374.810 kb on + strand, within DvMF_0321 at 374.855 kb on + strand, within DvMF_0321 at 375.058 kb on + strand, within DvMF_0321 at 375.580 kb on + strand, within DvMF_0322 at 375.713 kb on - strand, within DvMF_0322 at 376.676 kb on - strand, within DvMF_0323 at 376.828 kb on - strand, within DvMF_0323 at 376.981 kb on + strand at 377.019 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.125 mg/ml remove 374,657 - DvMF_0321 0.51 -0.3 374,657 - DvMF_0321 0.51 -2.0 374,708 - DvMF_0321 0.55 -0.7 374,720 + DvMF_0321 0.56 -0.8 374,810 + DvMF_0321 0.64 -1.0 374,855 + DvMF_0321 0.68 -0.2 375,058 + DvMF_0321 0.85 -0.8 375,580 + DvMF_0322 0.11 +4.0 375,713 - DvMF_0322 0.35 +4.1 376,676 - DvMF_0323 0.61 +4.1 376,828 - DvMF_0323 0.77 +4.2 376,981 + +0.6 377,019 - +3.1
Or see this region's nucleotide sequence