Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_B2615 and parB are separated by 102 nucleotides parB and parA overlap by 4 nucleotides parA and sepA2 are separated by 133 nucleotides
Synpcc7942_B2615: Synpcc7942_B2615 - hypothetical protein, at 65 to 394
_B2615
Synpcc7942_B2626: parB - hypothetical protein, at 497 to 1,321
parB
Synpcc7942_B2637: parA - ParA-like protein, at 1,318 to 1,929
parA
Synpcc7942_B2648: sepA2 - hypothetical protein, at 2,063 to 2,329
sepA2
Position (kb) 0
1
2 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 0.018 kb on + strand at 0.042 kb on + strand at 0.051 kb on - strand at 0.052 kb on + strand at 0.053 kb on + strand at 0.060 kb on - strand at 0.060 kb on - strand at 0.070 kb on - strand at 0.070 kb on - strand at 0.071 kb on - strand at 0.072 kb on + strand at 0.077 kb on + strand at 0.085 kb on - strand at 0.088 kb on + strand at 0.096 kb on - strand at 0.133 kb on + strand, within Synpcc7942_B2615 at 0.133 kb on + strand, within Synpcc7942_B2615 at 0.133 kb on + strand, within Synpcc7942_B2615 at 0.133 kb on + strand, within Synpcc7942_B2615 at 0.141 kb on - strand, within Synpcc7942_B2615 at 0.141 kb on - strand, within Synpcc7942_B2615 at 0.143 kb on + strand, within Synpcc7942_B2615 at 0.143 kb on + strand, within Synpcc7942_B2615 at 0.143 kb on + strand, within Synpcc7942_B2615 at 0.151 kb on - strand, within Synpcc7942_B2615 at 0.151 kb on - strand, within Synpcc7942_B2615 at 0.151 kb on - strand, within Synpcc7942_B2615 at 0.151 kb on - strand, within Synpcc7942_B2615 at 0.151 kb on - strand, within Synpcc7942_B2615 at 0.158 kb on + strand, within Synpcc7942_B2615 at 0.158 kb on + strand, within Synpcc7942_B2615 at 0.166 kb on - strand, within Synpcc7942_B2615 at 0.207 kb on - strand, within Synpcc7942_B2615 at 0.207 kb on - strand, within Synpcc7942_B2615 at 0.214 kb on + strand, within Synpcc7942_B2615 at 0.221 kb on + strand, within Synpcc7942_B2615 at 0.221 kb on + strand, within Synpcc7942_B2615 at 0.221 kb on + strand, within Synpcc7942_B2615 at 0.229 kb on - strand, within Synpcc7942_B2615 at 0.229 kb on - strand at 0.229 kb on - strand, within Synpcc7942_B2615 at 0.229 kb on - strand, within Synpcc7942_B2615 at 0.229 kb on - strand, within Synpcc7942_B2615 at 0.229 kb on - strand, within Synpcc7942_B2615 at 0.240 kb on + strand, within Synpcc7942_B2615 at 0.247 kb on + strand, within Synpcc7942_B2615 at 0.247 kb on + strand, within Synpcc7942_B2615 at 0.248 kb on - strand, within Synpcc7942_B2615 at 0.255 kb on - strand, within Synpcc7942_B2615 at 0.255 kb on - strand, within Synpcc7942_B2615 at 0.255 kb on - strand, within Synpcc7942_B2615 at 0.255 kb on - strand, within Synpcc7942_B2615 at 0.255 kb on - strand, within Synpcc7942_B2615 at 0.257 kb on + strand, within Synpcc7942_B2615 at 0.257 kb on + strand, within Synpcc7942_B2615 at 0.257 kb on + strand, within Synpcc7942_B2615 at 0.304 kb on - strand, within Synpcc7942_B2615 at 0.322 kb on + strand, within Synpcc7942_B2615 at 0.360 kb on + strand, within Synpcc7942_B2615 at 0.360 kb on + strand, within Synpcc7942_B2615 at 0.360 kb on + strand, within Synpcc7942_B2615 at 0.373 kb on + strand at 0.373 kb on + strand at 0.373 kb on + strand at 0.373 kb on + strand at 0.373 kb on + strand at 0.373 kb on + strand at 0.373 kb on + strand at 0.373 kb on + strand at 0.373 kb on + strand at 0.373 kb on + strand at 0.381 kb on - strand at 0.381 kb on - strand at 0.381 kb on - strand at 0.381 kb on - strand at 0.381 kb on - strand at 0.381 kb on - strand at 0.396 kb on + strand at 0.401 kb on + strand at 0.401 kb on + strand at 0.401 kb on + strand at 0.401 kb on + strand at 0.401 kb on + strand at 0.404 kb on - strand at 0.404 kb on - strand at 0.407 kb on - strand at 0.409 kb on - strand at 0.409 kb on - strand at 0.409 kb on - strand at 0.414 kb on - strand at 0.414 kb on - strand at 0.414 kb on - strand at 0.431 kb on + strand at 0.495 kb on + strand at 0.495 kb on + strand at 0.495 kb on + strand at 0.495 kb on + strand at 0.510 kb on + strand at 0.512 kb on + strand at 0.514 kb on + strand at 0.565 kb on - strand at 0.601 kb on - strand, within parB at 0.651 kb on + strand, within parB at 0.680 kb on + strand, within parB at 0.748 kb on + strand, within parB at 0.756 kb on - strand, within parB at 0.855 kb on + strand, within parB at 0.877 kb on - strand, within parB at 1.007 kb on + strand, within parB at 1.192 kb on - strand, within parB at 1.218 kb on + strand, within parB at 1.293 kb on - strand at 1.293 kb on - strand at 1.295 kb on + strand at 1.303 kb on - strand at 1.340 kb on - strand at 1.362 kb on - strand at 1.380 kb on + strand, within parA at 1.380 kb on + strand, within parA at 1.382 kb on - strand, within parA at 1.435 kb on + strand, within parA at 1.473 kb on + strand, within parA at 1.514 kb on + strand, within parA at 1.516 kb on - strand at 1.567 kb on + strand, within parA at 1.573 kb on - strand, within parA at 1.630 kb on + strand, within parA at 1.643 kb on - strand, within parA at 1.657 kb on - strand, within parA at 1.678 kb on - strand, within parA at 1.691 kb on - strand, within parA at 1.710 kb on - strand, within parA at 1.710 kb on - strand at 1.756 kb on + strand, within parA at 1.756 kb on + strand, within parA at 1.761 kb on + strand, within parA at 1.769 kb on - strand, within parA at 1.770 kb on + strand, within parA at 1.771 kb on - strand, within parA at 1.820 kb on + strand, within parA at 1.852 kb on + strand, within parA at 1.852 kb on + strand, within parA at 1.878 kb on + strand at 1.878 kb on + strand at 1.888 kb on + strand at 1.913 kb on + strand at 1.921 kb on - strand at 1.923 kb on + strand at 1.923 kb on + strand at 1.923 kb on + strand at 1.924 kb on + strand at 1.931 kb on - strand at 1.931 kb on - strand at 1.935 kb on + strand at 1.935 kb on + strand at 1.935 kb on + strand at 1.935 kb on + strand at 1.936 kb on + strand at 1.936 kb on + strand at 1.936 kb on + strand at 1.936 kb on + strand at 1.936 kb on + strand at 1.936 kb on + strand at 1.936 kb on + strand at 1.943 kb on - strand at 1.943 kb on - strand at 1.944 kb on - strand at 1.944 kb on - strand at 1.944 kb on - strand at 1.944 kb on - strand at 1.944 kb on - strand at 1.960 kb on + strand at 1.968 kb on - strand at 1.972 kb on + strand at 1.973 kb on + strand at 1.979 kb on + strand at 1.979 kb on + strand at 1.979 kb on + strand at 1.980 kb on - strand at 1.981 kb on - strand at 1.987 kb on - strand at 1.999 kb on + strand at 1.999 kb on + strand at 1.999 kb on + strand at 1.999 kb on + strand at 1.999 kb on + strand at 1.999 kb on + strand at 2.005 kb on - strand at 2.005 kb on - strand at 2.007 kb on - strand at 2.007 kb on - strand at 2.007 kb on - strand at 2.007 kb on - strand at 2.007 kb on - strand at 2.007 kb on - strand at 2.007 kb on - strand at 2.024 kb on + strand at 2.024 kb on + strand at 2.024 kb on + strand at 2.037 kb on + strand at 2.058 kb on - strand at 2.311 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 18 + -0.3 42 + +0.4 51 - +0.7 52 + +0.6 53 + +0.4 60 - +1.1 60 - -2.8 70 - +0.4 70 - +1.0 71 - -0.4 72 + -0.8 77 + +0.4 85 - +0.8 88 + +0.7 96 - +0.6 133 + Synpcc7942_B2615 0.21 -0.0 133 + Synpcc7942_B2615 0.21 +0.5 133 + Synpcc7942_B2615 0.21 -0.1 133 + Synpcc7942_B2615 0.21 +0.2 141 - Synpcc7942_B2615 0.23 +0.2 141 - Synpcc7942_B2615 0.23 +0.9 143 + Synpcc7942_B2615 0.24 +1.1 143 + Synpcc7942_B2615 0.24 -0.4 143 + Synpcc7942_B2615 0.24 +0.5 151 - Synpcc7942_B2615 0.26 +0.4 151 - Synpcc7942_B2615 0.26 +0.5 151 - Synpcc7942_B2615 0.26 -2.1 151 - Synpcc7942_B2615 0.26 -0.0 151 - Synpcc7942_B2615 0.26 +0.3 158 + Synpcc7942_B2615 0.28 -1.0 158 + Synpcc7942_B2615 0.28 +0.8 166 - Synpcc7942_B2615 0.31 +0.1 207 - Synpcc7942_B2615 0.43 +2.3 207 - Synpcc7942_B2615 0.43 -0.1 214 + Synpcc7942_B2615 0.45 -2.5 221 + Synpcc7942_B2615 0.47 +0.6 221 + Synpcc7942_B2615 0.47 -1.5 221 + Synpcc7942_B2615 0.47 -0.4 229 - Synpcc7942_B2615 0.50 -0.2 229 - -0.5 229 - Synpcc7942_B2615 0.50 +0.6 229 - Synpcc7942_B2615 0.50 +0.6 229 - Synpcc7942_B2615 0.50 +0.0 229 - Synpcc7942_B2615 0.50 -1.9 240 + Synpcc7942_B2615 0.53 +1.2 247 + Synpcc7942_B2615 0.55 +1.0 247 + Synpcc7942_B2615 0.55 +0.5 248 - Synpcc7942_B2615 0.55 -1.5 255 - Synpcc7942_B2615 0.58 -1.0 255 - Synpcc7942_B2615 0.58 +0.5 255 - Synpcc7942_B2615 0.58 +2.2 255 - Synpcc7942_B2615 0.58 +1.2 255 - Synpcc7942_B2615 0.58 +0.1 257 + Synpcc7942_B2615 0.58 +1.5 257 + Synpcc7942_B2615 0.58 +0.9 257 + Synpcc7942_B2615 0.58 +1.7 304 - Synpcc7942_B2615 0.72 -1.2 322 + Synpcc7942_B2615 0.78 +0.1 360 + Synpcc7942_B2615 0.89 +0.7 360 + Synpcc7942_B2615 0.89 +0.4 360 + Synpcc7942_B2615 0.89 -0.2 373 + +1.9 373 + +0.7 373 + +1.0 373 + +0.9 373 + +0.9 373 + +1.5 373 + +1.6 373 + +1.2 373 + +1.9 373 + +0.2 381 - +2.4 381 - +2.9 381 - +0.7 381 - +1.9 381 - +1.4 381 - +1.8 396 + +0.4 401 + +0.9 401 + +0.5 401 + -0.4 401 + -0.3 401 + +1.5 404 - +0.5 404 - -0.5 407 - -0.1 409 - -0.3 409 - -1.0 409 - +0.0 414 - +1.5 414 - -0.1 414 - -0.1 431 + +0.3 495 + -0.1 495 + +0.8 495 + +0.1 495 + -1.0 510 + +0.2 512 + -1.1 514 + +0.5 565 - -1.9 601 - parB Synpcc7942_B2626 0.13 -0.1 651 + parB Synpcc7942_B2626 0.19 -2.4 680 + parB Synpcc7942_B2626 0.22 -0.1 748 + parB Synpcc7942_B2626 0.30 -1.1 756 - parB Synpcc7942_B2626 0.31 -0.5 855 + parB Synpcc7942_B2626 0.43 -1.7 877 - parB Synpcc7942_B2626 0.46 -0.1 1,007 + parB Synpcc7942_B2626 0.62 -1.5 1,192 - parB Synpcc7942_B2626 0.84 +0.1 1,218 + parB Synpcc7942_B2626 0.87 -1.6 1,293 - -1.7 1,293 - -1.7 1,295 + +0.9 1,303 - +0.6 1,340 - +0.6 1,362 - +1.7 1,380 + parA Synpcc7942_B2637 0.10 -1.5 1,380 + parA Synpcc7942_B2637 0.10 -1.0 1,382 - parA Synpcc7942_B2637 0.10 -0.9 1,435 + parA Synpcc7942_B2637 0.19 -1.0 1,473 + parA Synpcc7942_B2637 0.25 -2.5 1,514 + parA Synpcc7942_B2637 0.32 -0.7 1,516 - -0.1 1,567 + parA Synpcc7942_B2637 0.41 +0.5 1,573 - parA Synpcc7942_B2637 0.42 -1.0 1,630 + parA Synpcc7942_B2637 0.51 -1.8 1,643 - parA Synpcc7942_B2637 0.53 -1.8 1,657 - parA Synpcc7942_B2637 0.55 -0.4 1,678 - parA Synpcc7942_B2637 0.59 -2.1 1,691 - parA Synpcc7942_B2637 0.61 -0.4 1,710 - parA Synpcc7942_B2637 0.64 -1.9 1,710 - +0.1 1,756 + parA Synpcc7942_B2637 0.72 -0.1 1,756 + parA Synpcc7942_B2637 0.72 -0.4 1,761 + parA Synpcc7942_B2637 0.72 +0.5 1,769 - parA Synpcc7942_B2637 0.74 -0.1 1,770 + parA Synpcc7942_B2637 0.74 -0.9 1,771 - parA Synpcc7942_B2637 0.74 -0.4 1,820 + parA Synpcc7942_B2637 0.82 +0.0 1,852 + parA Synpcc7942_B2637 0.87 -0.3 1,852 + parA Synpcc7942_B2637 0.87 -1.1 1,878 + -0.7 1,878 + -0.1 1,888 + +0.1 1,913 + -1.3 1,921 - +0.5 1,923 + -0.5 1,923 + -0.4 1,923 + +0.8 1,924 + -0.6 1,931 - +0.4 1,931 - -0.9 1,935 + -0.3 1,935 + -2.6 1,935 + -0.8 1,935 + -0.7 1,936 + -0.5 1,936 + -0.2 1,936 + -2.9 1,936 + +0.5 1,936 + +0.5 1,936 + -1.2 1,936 + -3.3 1,943 - -0.4 1,943 - +0.1 1,944 - -0.1 1,944 - +1.1 1,944 - -2.9 1,944 - -1.1 1,944 - -1.1 1,960 + -0.2 1,968 - +0.6 1,972 + -0.2 1,973 + +0.1 1,979 + -1.7 1,979 + +0.5 1,979 + -0.1 1,980 - -0.6 1,981 - -0.3 1,987 - -0.4 1,999 + -0.5 1,999 + -0.9 1,999 + -0.7 1,999 + -1.0 1,999 + -0.5 1,999 + +0.6 2,005 - +0.3 2,005 - +0.3 2,007 - -0.9 2,007 - -0.8 2,007 - +0.5 2,007 - +0.1 2,007 - -1.5 2,007 - +0.5 2,007 - -0.4 2,024 + -0.1 2,024 + +1.0 2,024 + -0.6 2,037 + -1.7 2,058 - -0.3 2,311 + +0.5
Or see this region's nucleotide sequence