Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_2515 and tRNA-Ala are separated by 124 nucleotides tRNA-Ala and Synpcc7942_2516 are separated by 62 nucleotides Synpcc7942_2516 and Synpcc7942_2517 are separated by 72 nucleotides Synpcc7942_2517 and glgA are separated by 21 nucleotides
Synpcc7942_2515: Synpcc7942_2515 - putative DNA helicase, at 2,594,192 to 2,596,459
_2515
Synpcc7942_R0018: tRNA-Ala - tRNA-Ala, at 2,596,584 to 2,596,656
tRNA-Ala
Synpcc7942_2516: Synpcc7942_2516 - hypothetical protein, at 2,596,719 to 2,596,958
_2516
Synpcc7942_2517: Synpcc7942_2517 - hypothetical protein, at 2,597,031 to 2,597,729
_2517
Synpcc7942_2518: glgA - glycogen synthase, at 2,597,751 to 2,599,148
glgA
Position (kb)
2597
2598 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2596.065 kb on - strand, within Synpcc7942_2515 at 2596.088 kb on + strand, within Synpcc7942_2515 at 2596.103 kb on - strand, within Synpcc7942_2515 at 2596.112 kb on - strand, within Synpcc7942_2515 at 2596.122 kb on - strand, within Synpcc7942_2515 at 2596.125 kb on + strand, within Synpcc7942_2515 at 2596.151 kb on + strand, within Synpcc7942_2515 at 2596.157 kb on + strand, within Synpcc7942_2515 at 2596.158 kb on - strand, within Synpcc7942_2515 at 2596.159 kb on - strand, within Synpcc7942_2515 at 2596.159 kb on - strand, within Synpcc7942_2515 at 2596.165 kb on - strand, within Synpcc7942_2515 at 2596.174 kb on - strand, within Synpcc7942_2515 at 2596.225 kb on + strand, within Synpcc7942_2515 at 2596.225 kb on + strand, within Synpcc7942_2515 at 2596.225 kb on + strand, within Synpcc7942_2515 at 2596.225 kb on + strand, within Synpcc7942_2515 at 2596.225 kb on + strand, within Synpcc7942_2515 at 2596.225 kb on + strand, within Synpcc7942_2515 at 2596.225 kb on + strand, within Synpcc7942_2515 at 2596.225 kb on + strand, within Synpcc7942_2515 at 2596.225 kb on + strand, within Synpcc7942_2515 at 2596.233 kb on - strand at 2596.233 kb on - strand at 2596.233 kb on - strand at 2596.233 kb on - strand at 2596.233 kb on - strand at 2596.233 kb on - strand at 2596.233 kb on - strand at 2596.247 kb on + strand at 2596.257 kb on + strand at 2596.257 kb on - strand at 2596.267 kb on + strand at 2596.291 kb on + strand at 2596.291 kb on + strand at 2596.299 kb on - strand at 2596.299 kb on - strand at 2596.340 kb on + strand at 2596.345 kb on + strand at 2596.348 kb on - strand at 2596.449 kb on - strand at 2596.483 kb on - strand at 2596.485 kb on + strand at 2596.485 kb on - strand at 2596.485 kb on - strand at 2596.503 kb on - strand at 2596.517 kb on - strand at 2596.522 kb on - strand at 2596.594 kb on - strand, within tRNA-Ala at 2596.728 kb on + strand at 2596.823 kb on + strand, within Synpcc7942_2516 at 2596.834 kb on + strand, within Synpcc7942_2516 at 2596.862 kb on - strand, within Synpcc7942_2516 at 2596.869 kb on + strand, within Synpcc7942_2516 at 2596.887 kb on - strand, within Synpcc7942_2516 at 2596.901 kb on + strand, within Synpcc7942_2516 at 2596.982 kb on - strand at 2596.985 kb on + strand at 2596.986 kb on + strand at 2596.993 kb on - strand at 2596.993 kb on - strand at 2596.993 kb on - strand at 2596.993 kb on - strand at 2597.001 kb on + strand at 2597.001 kb on - strand at 2597.008 kb on + strand at 2597.008 kb on + strand at 2597.008 kb on + strand at 2597.013 kb on + strand at 2597.018 kb on + strand at 2597.021 kb on - strand at 2597.077 kb on + strand at 2597.141 kb on - strand, within Synpcc7942_2517 at 2597.144 kb on + strand, within Synpcc7942_2517 at 2597.183 kb on + strand, within Synpcc7942_2517 at 2597.183 kb on + strand, within Synpcc7942_2517 at 2597.216 kb on + strand, within Synpcc7942_2517 at 2597.272 kb on - strand, within Synpcc7942_2517 at 2597.287 kb on + strand, within Synpcc7942_2517 at 2597.427 kb on + strand, within Synpcc7942_2517 at 2597.454 kb on + strand at 2597.459 kb on + strand, within Synpcc7942_2517 at 2597.467 kb on - strand, within Synpcc7942_2517 at 2597.478 kb on + strand, within Synpcc7942_2517 at 2597.535 kb on + strand, within Synpcc7942_2517 at 2597.535 kb on + strand, within Synpcc7942_2517 at 2597.535 kb on + strand, within Synpcc7942_2517 at 2597.543 kb on - strand, within Synpcc7942_2517 at 2597.543 kb on - strand, within Synpcc7942_2517 at 2597.554 kb on + strand, within Synpcc7942_2517 at 2597.558 kb on - strand, within Synpcc7942_2517 at 2597.599 kb on - strand, within Synpcc7942_2517 at 2597.724 kb on + strand at 2597.732 kb on - strand at 2597.732 kb on - strand at 2597.748 kb on + strand at 2597.762 kb on + strand at 2597.778 kb on - strand at 2597.783 kb on - strand at 2597.794 kb on + strand at 2597.794 kb on + strand at 2597.794 kb on + strand at 2597.794 kb on + strand at 2597.794 kb on + strand at 2597.794 kb on + strand at 2597.795 kb on - strand at 2597.802 kb on - strand at 2597.811 kb on + strand at 2597.916 kb on + strand, within glgA at 2598.471 kb on + strand, within glgA at 2598.589 kb on + strand, within glgA at 2598.707 kb on - strand, within glgA
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 2,596,065 - Synpcc7942_2515 0.83 +0.9 2,596,088 + Synpcc7942_2515 0.84 +0.6 2,596,103 - Synpcc7942_2515 0.84 -1.8 2,596,112 - Synpcc7942_2515 0.85 +0.7 2,596,122 - Synpcc7942_2515 0.85 -1.2 2,596,125 + Synpcc7942_2515 0.85 -1.3 2,596,151 + Synpcc7942_2515 0.86 +1.5 2,596,157 + Synpcc7942_2515 0.87 +2.1 2,596,158 - Synpcc7942_2515 0.87 -0.2 2,596,159 - Synpcc7942_2515 0.87 -0.7 2,596,159 - Synpcc7942_2515 0.87 -1.0 2,596,165 - Synpcc7942_2515 0.87 +1.1 2,596,174 - Synpcc7942_2515 0.87 -0.4 2,596,225 + Synpcc7942_2515 0.90 -0.5 2,596,225 + Synpcc7942_2515 0.90 -1.8 2,596,225 + Synpcc7942_2515 0.90 -1.3 2,596,225 + Synpcc7942_2515 0.90 +0.2 2,596,225 + Synpcc7942_2515 0.90 -0.4 2,596,225 + Synpcc7942_2515 0.90 -0.0 2,596,225 + Synpcc7942_2515 0.90 -0.2 2,596,225 + Synpcc7942_2515 0.90 +0.5 2,596,225 + Synpcc7942_2515 0.90 -0.2 2,596,233 - +0.6 2,596,233 - -0.7 2,596,233 - +0.3 2,596,233 - +0.4 2,596,233 - -0.2 2,596,233 - -0.2 2,596,233 - +1.3 2,596,247 + -0.3 2,596,257 + -0.4 2,596,257 - +0.5 2,596,267 + +0.8 2,596,291 + -0.2 2,596,291 + -0.2 2,596,299 - -0.4 2,596,299 - -2.1 2,596,340 + -0.5 2,596,345 + +0.7 2,596,348 - +0.4 2,596,449 - +1.1 2,596,483 - +0.5 2,596,485 + +0.4 2,596,485 - +0.7 2,596,485 - +0.4 2,596,503 - +0.5 2,596,517 - +0.8 2,596,522 - +0.7 2,596,594 - tRNA-Ala Synpcc7942_R0018 0.14 +1.3 2,596,728 + +0.3 2,596,823 + Synpcc7942_2516 0.43 +0.9 2,596,834 + Synpcc7942_2516 0.48 -0.1 2,596,862 - Synpcc7942_2516 0.60 -1.0 2,596,869 + Synpcc7942_2516 0.62 -0.2 2,596,887 - Synpcc7942_2516 0.70 -0.0 2,596,901 + Synpcc7942_2516 0.76 -0.1 2,596,982 - +0.4 2,596,985 + -0.5 2,596,986 + -0.4 2,596,993 - -0.3 2,596,993 - +0.3 2,596,993 - -1.1 2,596,993 - -1.3 2,597,001 + -0.9 2,597,001 - -1.9 2,597,008 + +0.9 2,597,008 + -0.1 2,597,008 + +0.3 2,597,013 + -0.2 2,597,018 + +1.0 2,597,021 - +0.3 2,597,077 + -0.6 2,597,141 - Synpcc7942_2517 0.16 -2.2 2,597,144 + Synpcc7942_2517 0.16 -0.5 2,597,183 + Synpcc7942_2517 0.22 +0.1 2,597,183 + Synpcc7942_2517 0.22 -1.0 2,597,216 + Synpcc7942_2517 0.26 -1.1 2,597,272 - Synpcc7942_2517 0.34 -1.4 2,597,287 + Synpcc7942_2517 0.37 -0.6 2,597,427 + Synpcc7942_2517 0.57 -2.6 2,597,454 + -1.1 2,597,459 + Synpcc7942_2517 0.61 -0.7 2,597,467 - Synpcc7942_2517 0.62 -2.9 2,597,478 + Synpcc7942_2517 0.64 -2.5 2,597,535 + Synpcc7942_2517 0.72 -0.4 2,597,535 + Synpcc7942_2517 0.72 -1.3 2,597,535 + Synpcc7942_2517 0.72 -2.7 2,597,543 - Synpcc7942_2517 0.73 -1.5 2,597,543 - Synpcc7942_2517 0.73 +0.2 2,597,554 + Synpcc7942_2517 0.75 +0.2 2,597,558 - Synpcc7942_2517 0.75 -0.7 2,597,599 - Synpcc7942_2517 0.81 -2.1 2,597,724 + +0.2 2,597,732 - +0.5 2,597,732 - +0.0 2,597,748 + -0.9 2,597,762 + +1.3 2,597,778 - +0.8 2,597,783 - +2.2 2,597,794 + +1.2 2,597,794 + +1.7 2,597,794 + +1.5 2,597,794 + -0.8 2,597,794 + +2.0 2,597,794 + +1.9 2,597,795 - +2.5 2,597,802 - +1.3 2,597,811 + -2.5 2,597,916 + glgA Synpcc7942_2518 0.12 -1.8 2,598,471 + glgA Synpcc7942_2518 0.52 +1.8 2,598,589 + glgA Synpcc7942_2518 0.60 -2.7 2,598,707 - glgA Synpcc7942_2518 0.68 -3.0
Or see this region's nucleotide sequence