Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt crtR and pnp are separated by 272 nucleotides pnp and pstB are separated by 161 nucleotides
Synpcc7942_2439: crtR - beta-carotene hydroxylase, at 2,514,276 to 2,515,271
crtR
Synpcc7942_2440: pnp - polynucleotide phosphorylase/polyadenylase, at 2,515,544 to 2,517,694
pnp
Synpcc7942_2441: pstB - Phosphate transport system permease protein 1, at 2,517,856 to 2,518,650
pstB
Position (kb)
2515
2516
2517
2518 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2515.164 kb on - strand at 2515.179 kb on - strand at 2515.182 kb on + strand at 2515.209 kb on - strand at 2515.265 kb on - strand at 2515.265 kb on - strand at 2515.304 kb on - strand at 2515.306 kb on + strand at 2515.306 kb on + strand at 2515.318 kb on - strand at 2515.340 kb on + strand at 2515.348 kb on - strand at 2515.348 kb on - strand at 2515.348 kb on - strand at 2515.388 kb on + strand at 2515.388 kb on - strand at 2515.416 kb on - strand at 2515.417 kb on - strand at 2515.427 kb on - strand at 2515.468 kb on + strand at 2515.527 kb on + strand at 2515.540 kb on - strand at 2515.543 kb on + strand at 2515.572 kb on + strand at 2516.027 kb on - strand, within pnp at 2516.153 kb on - strand, within pnp at 2516.870 kb on + strand, within pnp at 2516.870 kb on + strand, within pnp at 2516.878 kb on - strand, within pnp at 2516.878 kb on - strand, within pnp at 2517.028 kb on - strand, within pnp at 2517.143 kb on - strand, within pnp at 2517.589 kb on + strand at 2517.597 kb on - strand at 2517.620 kb on - strand at 2517.620 kb on - strand at 2517.717 kb on + strand at 2517.754 kb on - strand at 2517.756 kb on - strand at 2517.781 kb on - strand at 2517.809 kb on + strand at 2517.817 kb on - strand at 2517.833 kb on - strand at 2517.833 kb on - strand at 2517.837 kb on + strand at 2517.854 kb on - strand at 2517.854 kb on - strand at 2518.673 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 2,515,164 - +1.2 2,515,179 - +0.6 2,515,182 + +0.9 2,515,209 - +0.3 2,515,265 - +0.0 2,515,265 - +0.1 2,515,304 - +0.0 2,515,306 + +1.0 2,515,306 + -1.2 2,515,318 - +0.1 2,515,340 + -2.2 2,515,348 - -0.5 2,515,348 - +0.3 2,515,348 - -0.1 2,515,388 + -0.3 2,515,388 - -1.1 2,515,416 - -0.8 2,515,417 - -0.1 2,515,427 - -0.1 2,515,468 + +0.4 2,515,527 + +1.0 2,515,540 - +1.2 2,515,543 + +1.4 2,515,572 + +1.0 2,516,027 - pnp Synpcc7942_2440 0.22 -1.2 2,516,153 - pnp Synpcc7942_2440 0.28 +0.8 2,516,870 + pnp Synpcc7942_2440 0.62 -1.0 2,516,870 + pnp Synpcc7942_2440 0.62 -1.3 2,516,878 - pnp Synpcc7942_2440 0.62 -0.7 2,516,878 - pnp Synpcc7942_2440 0.62 -3.6 2,517,028 - pnp Synpcc7942_2440 0.69 -1.5 2,517,143 - pnp Synpcc7942_2440 0.74 -1.1 2,517,589 + -2.4 2,517,597 - +0.5 2,517,620 - -1.5 2,517,620 - -0.7 2,517,717 + +1.4 2,517,754 - -0.0 2,517,756 - -0.9 2,517,781 - -0.8 2,517,809 + -0.5 2,517,817 - +0.9 2,517,833 - -0.6 2,517,833 - +1.0 2,517,837 + +1.7 2,517,854 - +1.5 2,517,854 - -0.4 2,518,673 + +1.3
Or see this region's nucleotide sequence