Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisB and Synpcc7942_2347 overlap by 4 nucleotides Synpcc7942_2347 and queA are separated by 18 nucleotides
Synpcc7942_2346: hisB - HAD-superfamily subfamily IA, at 2,413,356 to 2,414,141
hisB
Synpcc7942_2347: Synpcc7942_2347 - hypothetical protein, at 2,414,138 to 2,415,214
_2347
Synpcc7942_2348: queA - S-adenosylmethionine:tRNA ribosyltransferase-isomerase, at 2,415,233 to 2,416,312
queA
Position (kb)
2414
2415
2416 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2413.217 kb on + strand at 2413.291 kb on - strand at 2413.305 kb on + strand at 2413.327 kb on + strand at 2413.327 kb on + strand at 2413.327 kb on + strand at 2413.327 kb on + strand at 2413.327 kb on + strand at 2413.335 kb on - strand at 2413.335 kb on - strand at 2413.335 kb on - strand at 2413.335 kb on - strand at 2413.335 kb on - strand at 2413.335 kb on - strand at 2413.338 kb on - strand at 2413.340 kb on + strand at 2413.341 kb on + strand at 2413.351 kb on + strand at 2413.354 kb on + strand at 2413.356 kb on - strand at 2413.359 kb on - strand at 2413.359 kb on - strand at 2413.359 kb on - strand at 2413.364 kb on + strand at 2413.364 kb on + strand at 2413.364 kb on + strand at 2414.120 kb on + strand at 2414.120 kb on + strand at 2414.138 kb on - strand at 2414.138 kb on - strand at 2414.138 kb on - strand at 2414.151 kb on + strand at 2414.193 kb on - strand at 2414.202 kb on + strand at 2414.251 kb on - strand, within Synpcc7942_2347 at 2414.251 kb on - strand, within Synpcc7942_2347 at 2414.251 kb on - strand, within Synpcc7942_2347 at 2414.251 kb on - strand, within Synpcc7942_2347 at 2414.266 kb on + strand, within Synpcc7942_2347 at 2414.266 kb on + strand, within Synpcc7942_2347 at 2414.301 kb on + strand, within Synpcc7942_2347 at 2414.301 kb on + strand at 2414.309 kb on - strand, within Synpcc7942_2347 at 2414.336 kb on + strand, within Synpcc7942_2347 at 2414.361 kb on + strand, within Synpcc7942_2347 at 2414.361 kb on + strand at 2414.365 kb on + strand, within Synpcc7942_2347 at 2414.365 kb on + strand, within Synpcc7942_2347 at 2414.369 kb on - strand, within Synpcc7942_2347 at 2414.462 kb on + strand at 2414.522 kb on + strand, within Synpcc7942_2347 at 2414.530 kb on - strand, within Synpcc7942_2347 at 2414.552 kb on + strand, within Synpcc7942_2347 at 2414.616 kb on + strand, within Synpcc7942_2347 at 2414.644 kb on + strand at 2414.679 kb on + strand, within Synpcc7942_2347 at 2414.679 kb on + strand, within Synpcc7942_2347 at 2414.679 kb on + strand, within Synpcc7942_2347 at 2414.681 kb on + strand, within Synpcc7942_2347 at 2414.681 kb on + strand, within Synpcc7942_2347 at 2414.699 kb on - strand, within Synpcc7942_2347 at 2414.734 kb on + strand, within Synpcc7942_2347 at 2414.737 kb on + strand, within Synpcc7942_2347 at 2414.742 kb on - strand, within Synpcc7942_2347 at 2414.742 kb on - strand, within Synpcc7942_2347 at 2414.788 kb on + strand, within Synpcc7942_2347 at 2414.788 kb on + strand, within Synpcc7942_2347 at 2414.806 kb on + strand, within Synpcc7942_2347 at 2414.806 kb on + strand, within Synpcc7942_2347 at 2414.821 kb on - strand, within Synpcc7942_2347 at 2414.837 kb on + strand, within Synpcc7942_2347 at 2414.861 kb on + strand, within Synpcc7942_2347 at 2414.895 kb on + strand, within Synpcc7942_2347 at 2414.912 kb on + strand at 2414.912 kb on + strand, within Synpcc7942_2347 at 2414.912 kb on + strand, within Synpcc7942_2347 at 2414.920 kb on - strand, within Synpcc7942_2347 at 2414.920 kb on - strand, within Synpcc7942_2347 at 2414.920 kb on - strand, within Synpcc7942_2347 at 2414.920 kb on - strand, within Synpcc7942_2347 at 2414.958 kb on - strand, within Synpcc7942_2347 at 2414.963 kb on + strand, within Synpcc7942_2347 at 2414.976 kb on + strand, within Synpcc7942_2347 at 2414.976 kb on - strand, within Synpcc7942_2347 at 2415.003 kb on + strand, within Synpcc7942_2347 at 2415.011 kb on - strand, within Synpcc7942_2347 at 2415.016 kb on - strand, within Synpcc7942_2347 at 2415.039 kb on - strand, within Synpcc7942_2347 at 2415.050 kb on - strand, within Synpcc7942_2347 at 2415.050 kb on - strand, within Synpcc7942_2347 at 2415.055 kb on - strand, within Synpcc7942_2347 at 2415.085 kb on - strand, within Synpcc7942_2347 at 2415.089 kb on + strand, within Synpcc7942_2347 at 2415.097 kb on - strand, within Synpcc7942_2347 at 2415.097 kb on - strand, within Synpcc7942_2347 at 2415.097 kb on - strand, within Synpcc7942_2347 at 2415.123 kb on + strand at 2415.123 kb on + strand at 2415.131 kb on - strand at 2415.140 kb on + strand at 2415.202 kb on + strand at 2415.210 kb on - strand at 2415.210 kb on - strand at 2415.210 kb on - strand at 2415.210 kb on - strand at 2415.216 kb on + strand at 2415.240 kb on + strand at 2415.248 kb on - strand at 2415.248 kb on - strand at 2415.288 kb on + strand at 2415.302 kb on + strand at 2415.302 kb on + strand at 2415.302 kb on + strand at 2415.302 kb on + strand at 2415.309 kb on + strand at 2415.310 kb on - strand at 2415.310 kb on - strand at 2415.317 kb on - strand at 2415.344 kb on + strand, within queA at 2415.439 kb on + strand, within queA at 2415.447 kb on - strand, within queA at 2415.447 kb on - strand, within queA at 2415.447 kb on - strand, within queA at 2415.447 kb on - strand, within queA at 2415.455 kb on - strand, within queA at 2415.487 kb on + strand, within queA at 2415.531 kb on - strand, within queA at 2415.564 kb on - strand, within queA at 2415.579 kb on + strand, within queA at 2415.579 kb on + strand, within queA at 2415.579 kb on + strand, within queA at 2415.587 kb on - strand, within queA at 2415.648 kb on + strand, within queA at 2415.682 kb on + strand, within queA at 2415.694 kb on + strand, within queA at 2415.703 kb on + strand, within queA at 2415.725 kb on + strand, within queA at 2415.731 kb on + strand, within queA at 2415.731 kb on + strand, within queA at 2415.734 kb on - strand, within queA at 2415.750 kb on + strand, within queA at 2415.750 kb on + strand, within queA at 2415.770 kb on + strand, within queA at 2415.770 kb on + strand, within queA at 2415.770 kb on + strand, within queA at 2415.778 kb on - strand, within queA at 2415.778 kb on - strand, within queA at 2415.797 kb on - strand, within queA at 2415.798 kb on - strand, within queA at 2415.812 kb on + strand, within queA at 2415.815 kb on - strand, within queA at 2415.825 kb on + strand, within queA at 2415.828 kb on - strand, within queA at 2415.842 kb on + strand, within queA at 2415.843 kb on + strand, within queA at 2415.849 kb on - strand, within queA at 2415.861 kb on - strand, within queA at 2415.869 kb on + strand, within queA at 2415.870 kb on - strand, within queA at 2415.881 kb on - strand, within queA at 2415.891 kb on + strand, within queA at 2415.894 kb on - strand, within queA at 2415.900 kb on - strand, within queA at 2415.909 kb on - strand, within queA at 2415.923 kb on - strand, within queA at 2415.923 kb on - strand, within queA at 2415.924 kb on + strand, within queA at 2415.927 kb on + strand, within queA at 2415.934 kb on + strand, within queA at 2416.027 kb on + strand, within queA at 2416.036 kb on - strand, within queA at 2416.056 kb on - strand, within queA at 2416.064 kb on + strand, within queA at 2416.064 kb on + strand, within queA at 2416.072 kb on - strand, within queA at 2416.075 kb on + strand, within queA at 2416.075 kb on + strand, within queA at 2416.075 kb on + strand, within queA at 2416.118 kb on + strand, within queA at 2416.122 kb on + strand, within queA at 2416.122 kb on + strand, within queA at 2416.192 kb on - strand, within queA at 2416.206 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 2,413,217 + -1.0 2,413,291 - -3.6 2,413,305 + +1.2 2,413,327 + -0.7 2,413,327 + -0.5 2,413,327 + -0.6 2,413,327 + -0.6 2,413,327 + -2.4 2,413,335 - +0.6 2,413,335 - +0.8 2,413,335 - -1.6 2,413,335 - +0.3 2,413,335 - -0.2 2,413,335 - -0.3 2,413,338 - +0.3 2,413,340 + -0.3 2,413,341 + -0.5 2,413,351 + -0.2 2,413,354 + +0.2 2,413,356 - -0.1 2,413,359 - -0.4 2,413,359 - +1.4 2,413,359 - +1.2 2,413,364 + -0.2 2,413,364 + -0.5 2,413,364 + +0.3 2,414,120 + -0.7 2,414,120 + +0.9 2,414,138 - -1.0 2,414,138 - -0.2 2,414,138 - -0.4 2,414,151 + +4.1 2,414,193 - +4.0 2,414,202 + +2.2 2,414,251 - Synpcc7942_2347 0.10 +3.9 2,414,251 - Synpcc7942_2347 0.10 +2.1 2,414,251 - Synpcc7942_2347 0.10 +4.1 2,414,251 - Synpcc7942_2347 0.10 +3.3 2,414,266 + Synpcc7942_2347 0.12 +3.5 2,414,266 + Synpcc7942_2347 0.12 +3.0 2,414,301 + Synpcc7942_2347 0.15 +4.2 2,414,301 + +3.8 2,414,309 - Synpcc7942_2347 0.16 +3.5 2,414,336 + Synpcc7942_2347 0.18 +3.9 2,414,361 + Synpcc7942_2347 0.21 +3.8 2,414,361 + +3.8 2,414,365 + Synpcc7942_2347 0.21 +4.1 2,414,365 + Synpcc7942_2347 0.21 +4.0 2,414,369 - Synpcc7942_2347 0.21 +4.3 2,414,462 + +3.4 2,414,522 + Synpcc7942_2347 0.36 +4.3 2,414,530 - Synpcc7942_2347 0.36 +4.3 2,414,552 + Synpcc7942_2347 0.38 +3.8 2,414,616 + Synpcc7942_2347 0.44 +4.0 2,414,644 + +3.8 2,414,679 + Synpcc7942_2347 0.50 +3.5 2,414,679 + Synpcc7942_2347 0.50 +3.1 2,414,679 + Synpcc7942_2347 0.50 +2.7 2,414,681 + Synpcc7942_2347 0.50 +3.3 2,414,681 + Synpcc7942_2347 0.50 +3.8 2,414,699 - Synpcc7942_2347 0.52 +4.1 2,414,734 + Synpcc7942_2347 0.55 +4.4 2,414,737 + Synpcc7942_2347 0.56 +3.8 2,414,742 - Synpcc7942_2347 0.56 +3.4 2,414,742 - Synpcc7942_2347 0.56 +3.5 2,414,788 + Synpcc7942_2347 0.60 +3.9 2,414,788 + Synpcc7942_2347 0.60 +4.0 2,414,806 + Synpcc7942_2347 0.62 +3.1 2,414,806 + Synpcc7942_2347 0.62 +4.4 2,414,821 - Synpcc7942_2347 0.63 +3.8 2,414,837 + Synpcc7942_2347 0.65 +3.8 2,414,861 + Synpcc7942_2347 0.67 +3.3 2,414,895 + Synpcc7942_2347 0.70 +3.3 2,414,912 + +4.2 2,414,912 + Synpcc7942_2347 0.72 +4.0 2,414,912 + Synpcc7942_2347 0.72 +3.8 2,414,920 - Synpcc7942_2347 0.73 +4.7 2,414,920 - Synpcc7942_2347 0.73 +4.3 2,414,920 - Synpcc7942_2347 0.73 +4.2 2,414,920 - Synpcc7942_2347 0.73 +3.3 2,414,958 - Synpcc7942_2347 0.76 +3.5 2,414,963 + Synpcc7942_2347 0.77 +4.1 2,414,976 + Synpcc7942_2347 0.78 +4.1 2,414,976 - Synpcc7942_2347 0.78 +3.8 2,415,003 + Synpcc7942_2347 0.80 +4.3 2,415,011 - Synpcc7942_2347 0.81 +3.6 2,415,016 - Synpcc7942_2347 0.82 +4.2 2,415,039 - Synpcc7942_2347 0.84 +3.9 2,415,050 - Synpcc7942_2347 0.85 +3.7 2,415,050 - Synpcc7942_2347 0.85 +3.6 2,415,055 - Synpcc7942_2347 0.85 +3.6 2,415,085 - Synpcc7942_2347 0.88 +3.8 2,415,089 + Synpcc7942_2347 0.88 +0.7 2,415,097 - Synpcc7942_2347 0.89 +3.6 2,415,097 - Synpcc7942_2347 0.89 +3.7 2,415,097 - Synpcc7942_2347 0.89 +3.4 2,415,123 + +0.2 2,415,123 + -0.2 2,415,131 - -0.3 2,415,140 + +1.0 2,415,202 + +0.0 2,415,210 - +1.5 2,415,210 - +0.8 2,415,210 - +1.4 2,415,210 - -0.6 2,415,216 + +2.4 2,415,240 + +1.4 2,415,248 - +1.0 2,415,248 - +2.3 2,415,288 + -0.7 2,415,302 + +0.1 2,415,302 + +1.5 2,415,302 + -0.4 2,415,302 + +0.9 2,415,309 + -0.2 2,415,310 - +1.0 2,415,310 - -1.2 2,415,317 - +3.3 2,415,344 + queA Synpcc7942_2348 0.10 +1.8 2,415,439 + queA Synpcc7942_2348 0.19 +1.2 2,415,447 - queA Synpcc7942_2348 0.20 +1.0 2,415,447 - queA Synpcc7942_2348 0.20 +0.3 2,415,447 - queA Synpcc7942_2348 0.20 +0.1 2,415,447 - queA Synpcc7942_2348 0.20 +2.4 2,415,455 - queA Synpcc7942_2348 0.21 +1.4 2,415,487 + queA Synpcc7942_2348 0.24 +1.9 2,415,531 - queA Synpcc7942_2348 0.28 +1.8 2,415,564 - queA Synpcc7942_2348 0.31 +1.3 2,415,579 + queA Synpcc7942_2348 0.32 +1.8 2,415,579 + queA Synpcc7942_2348 0.32 +1.2 2,415,579 + queA Synpcc7942_2348 0.32 +0.4 2,415,587 - queA Synpcc7942_2348 0.33 +0.1 2,415,648 + queA Synpcc7942_2348 0.38 +1.8 2,415,682 + queA Synpcc7942_2348 0.42 +1.6 2,415,694 + queA Synpcc7942_2348 0.43 +1.9 2,415,703 + queA Synpcc7942_2348 0.44 -0.5 2,415,725 + queA Synpcc7942_2348 0.46 -0.1 2,415,731 + queA Synpcc7942_2348 0.46 +0.7 2,415,731 + queA Synpcc7942_2348 0.46 +2.0 2,415,734 - queA Synpcc7942_2348 0.46 +1.4 2,415,750 + queA Synpcc7942_2348 0.48 +0.9 2,415,750 + queA Synpcc7942_2348 0.48 +1.6 2,415,770 + queA Synpcc7942_2348 0.50 -0.8 2,415,770 + queA Synpcc7942_2348 0.50 +2.0 2,415,770 + queA Synpcc7942_2348 0.50 +0.5 2,415,778 - queA Synpcc7942_2348 0.50 +1.6 2,415,778 - queA Synpcc7942_2348 0.50 -1.6 2,415,797 - queA Synpcc7942_2348 0.52 +0.3 2,415,798 - queA Synpcc7942_2348 0.52 -0.2 2,415,812 + queA Synpcc7942_2348 0.54 +2.2 2,415,815 - queA Synpcc7942_2348 0.54 +1.5 2,415,825 + queA Synpcc7942_2348 0.55 +1.5 2,415,828 - queA Synpcc7942_2348 0.55 +1.3 2,415,842 + queA Synpcc7942_2348 0.56 +0.7 2,415,843 + queA Synpcc7942_2348 0.56 +0.7 2,415,849 - queA Synpcc7942_2348 0.57 +0.5 2,415,861 - queA Synpcc7942_2348 0.58 +1.8 2,415,869 + queA Synpcc7942_2348 0.59 +2.3 2,415,870 - queA Synpcc7942_2348 0.59 -0.4 2,415,881 - queA Synpcc7942_2348 0.60 +1.9 2,415,891 + queA Synpcc7942_2348 0.61 +1.4 2,415,894 - queA Synpcc7942_2348 0.61 +2.2 2,415,900 - queA Synpcc7942_2348 0.62 +1.5 2,415,909 - queA Synpcc7942_2348 0.63 +0.2 2,415,923 - queA Synpcc7942_2348 0.64 +1.7 2,415,923 - queA Synpcc7942_2348 0.64 +2.0 2,415,924 + queA Synpcc7942_2348 0.64 +1.8 2,415,927 + queA Synpcc7942_2348 0.64 +0.5 2,415,934 + queA Synpcc7942_2348 0.65 +1.3 2,416,027 + queA Synpcc7942_2348 0.74 +1.0 2,416,036 - queA Synpcc7942_2348 0.74 +1.2 2,416,056 - queA Synpcc7942_2348 0.76 +1.9 2,416,064 + queA Synpcc7942_2348 0.77 +1.2 2,416,064 + queA Synpcc7942_2348 0.77 +1.9 2,416,072 - queA Synpcc7942_2348 0.78 +1.6 2,416,075 + queA Synpcc7942_2348 0.78 +1.0 2,416,075 + queA Synpcc7942_2348 0.78 +1.2 2,416,075 + queA Synpcc7942_2348 0.78 +1.3 2,416,118 + queA Synpcc7942_2348 0.82 +1.3 2,416,122 + queA Synpcc7942_2348 0.82 +1.1 2,416,122 + queA Synpcc7942_2348 0.82 +1.4 2,416,192 - queA Synpcc7942_2348 0.89 +0.9 2,416,206 + +1.5
Or see this region's nucleotide sequence