Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt petB and petD are separated by 96 nucleotides petD and opcA are separated by 110 nucleotides opcA and zwf are separated by 39 nucleotides
Synpcc7942_2331: petB - cytochrome b6, at 2,398,625 to 2,399,272
petB
Synpcc7942_2332: petD - cytochrome b6-f complex subunit 4, at 2,399,369 to 2,399,851
petD
Synpcc7942_2333: opcA - opcA protein, at 2,399,962 to 2,401,299
opcA
Synpcc7942_2334: zwf - glucose-6-phosphate 1-dehydrogenase, at 2,401,339 to 2,402,874
zwf
Position (kb)
2399
2400
2401
2402 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2399.845 kb on + strand at 2399.845 kb on + strand at 2399.869 kb on - strand at 2399.880 kb on + strand at 2399.940 kb on - strand at 2399.960 kb on + strand at 2399.977 kb on + strand at 2399.985 kb on + strand at 2399.993 kb on - strand at 2400.030 kb on + strand at 2400.040 kb on - strand at 2400.082 kb on + strand at 2400.094 kb on - strand at 2400.099 kb on - strand, within opcA at 2400.104 kb on - strand, within opcA at 2400.127 kb on - strand, within opcA at 2400.210 kb on + strand, within opcA at 2400.223 kb on + strand, within opcA at 2400.223 kb on + strand, within opcA at 2400.231 kb on - strand at 2400.231 kb on - strand, within opcA at 2400.269 kb on + strand, within opcA at 2400.277 kb on - strand, within opcA at 2400.296 kb on - strand, within opcA at 2400.316 kb on + strand, within opcA at 2400.343 kb on + strand, within opcA at 2400.388 kb on + strand, within opcA at 2400.396 kb on - strand, within opcA at 2400.448 kb on - strand, within opcA at 2400.458 kb on - strand, within opcA at 2400.500 kb on - strand, within opcA at 2400.576 kb on - strand, within opcA at 2400.586 kb on - strand, within opcA at 2400.691 kb on + strand, within opcA at 2400.699 kb on - strand, within opcA at 2400.713 kb on - strand, within opcA at 2400.714 kb on - strand, within opcA at 2400.763 kb on + strand, within opcA at 2400.789 kb on - strand, within opcA at 2400.789 kb on - strand, within opcA at 2400.797 kb on - strand, within opcA at 2400.808 kb on + strand, within opcA at 2400.810 kb on + strand, within opcA at 2400.851 kb on + strand, within opcA at 2400.853 kb on + strand, within opcA at 2400.858 kb on + strand, within opcA at 2400.866 kb on - strand, within opcA at 2400.908 kb on + strand, within opcA at 2400.908 kb on + strand, within opcA at 2400.908 kb on - strand, within opcA at 2400.915 kb on + strand, within opcA at 2400.915 kb on + strand, within opcA at 2400.923 kb on - strand, within opcA at 2400.933 kb on - strand, within opcA at 2400.938 kb on - strand, within opcA at 2400.938 kb on - strand, within opcA at 2400.948 kb on - strand, within opcA at 2400.958 kb on + strand, within opcA at 2400.963 kb on - strand, within opcA at 2400.966 kb on - strand, within opcA at 2400.966 kb on - strand, within opcA at 2400.976 kb on - strand, within opcA at 2400.986 kb on - strand, within opcA at 2400.995 kb on - strand, within opcA at 2401.016 kb on + strand, within opcA at 2401.016 kb on + strand, within opcA at 2401.016 kb on + strand, within opcA at 2401.022 kb on - strand, within opcA at 2401.024 kb on - strand, within opcA at 2401.024 kb on - strand, within opcA at 2401.090 kb on - strand, within opcA at 2401.102 kb on - strand, within opcA at 2401.107 kb on - strand, within opcA at 2401.113 kb on - strand, within opcA at 2401.139 kb on + strand, within opcA at 2401.145 kb on - strand, within opcA at 2401.168 kb on + strand at 2401.180 kb on - strand at 2401.190 kb on + strand at 2401.201 kb on - strand at 2401.211 kb on + strand at 2401.211 kb on + strand at 2401.211 kb on + strand at 2401.211 kb on + strand at 2401.214 kb on - strand at 2401.219 kb on - strand at 2401.219 kb on - strand at 2401.219 kb on - strand at 2401.381 kb on - strand at 2401.492 kb on + strand at 2401.500 kb on - strand, within zwf at 2401.507 kb on + strand, within zwf at 2401.507 kb on + strand, within zwf at 2401.507 kb on + strand, within zwf at 2401.515 kb on - strand, within zwf at 2401.515 kb on - strand, within zwf at 2401.515 kb on - strand, within zwf at 2401.537 kb on + strand, within zwf at 2401.540 kb on - strand, within zwf at 2401.545 kb on - strand, within zwf at 2401.552 kb on + strand, within zwf at 2401.553 kb on + strand, within zwf at 2401.568 kb on - strand, within zwf at 2401.584 kb on - strand, within zwf at 2401.602 kb on - strand, within zwf at 2401.607 kb on - strand, within zwf at 2401.609 kb on - strand, within zwf at 2401.631 kb on - strand, within zwf at 2401.654 kb on + strand, within zwf at 2401.665 kb on + strand, within zwf at 2401.667 kb on + strand, within zwf at 2401.667 kb on + strand, within zwf at 2401.673 kb on - strand, within zwf at 2401.691 kb on + strand, within zwf at 2401.697 kb on - strand, within zwf at 2401.697 kb on - strand, within zwf at 2401.698 kb on + strand, within zwf at 2401.698 kb on - strand, within zwf at 2401.706 kb on - strand, within zwf at 2401.751 kb on + strand, within zwf at 2401.761 kb on + strand, within zwf at 2401.779 kb on - strand, within zwf at 2401.792 kb on + strand, within zwf at 2401.793 kb on + strand, within zwf at 2401.800 kb on - strand, within zwf at 2401.803 kb on + strand, within zwf at 2401.803 kb on + strand, within zwf at 2401.829 kb on - strand, within zwf at 2401.829 kb on - strand, within zwf at 2401.837 kb on + strand, within zwf at 2401.861 kb on + strand, within zwf at 2401.872 kb on + strand, within zwf at 2401.879 kb on + strand, within zwf at 2401.879 kb on - strand, within zwf at 2401.890 kb on - strand, within zwf at 2401.905 kb on - strand, within zwf at 2401.920 kb on - strand, within zwf at 2401.943 kb on + strand, within zwf at 2401.943 kb on + strand, within zwf at 2401.943 kb on + strand, within zwf at 2401.943 kb on + strand, within zwf at 2401.943 kb on + strand, within zwf at 2401.943 kb on + strand, within zwf at 2401.943 kb on + strand, within zwf at 2401.943 kb on + strand, within zwf at 2401.951 kb on - strand, within zwf at 2401.951 kb on - strand, within zwf at 2401.951 kb on - strand, within zwf at 2401.956 kb on - strand, within zwf at 2402.010 kb on - strand, within zwf at 2402.012 kb on + strand, within zwf at 2402.012 kb on + strand, within zwf at 2402.020 kb on - strand, within zwf at 2402.020 kb on - strand, within zwf at 2402.020 kb on - strand, within zwf at 2402.051 kb on + strand, within zwf at 2402.051 kb on - strand, within zwf at 2402.053 kb on - strand, within zwf at 2402.053 kb on - strand, within zwf at 2402.083 kb on + strand, within zwf at 2402.086 kb on - strand, within zwf at 2402.098 kb on + strand, within zwf at 2402.103 kb on + strand, within zwf at 2402.106 kb on + strand, within zwf at 2402.107 kb on - strand, within zwf at 2402.110 kb on + strand, within zwf at 2402.142 kb on - strand, within zwf at 2402.173 kb on - strand, within zwf at 2402.173 kb on - strand, within zwf at 2402.179 kb on + strand, within zwf at 2402.182 kb on - strand, within zwf at 2402.187 kb on - strand, within zwf at 2402.198 kb on - strand, within zwf at 2402.203 kb on - strand, within zwf at 2402.217 kb on + strand, within zwf at 2402.278 kb on + strand, within zwf at 2402.284 kb on - strand, within zwf at 2402.284 kb on - strand, within zwf at 2402.285 kb on - strand, within zwf at 2402.299 kb on - strand, within zwf
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 2,399,845 + -0.5 2,399,845 + +0.5 2,399,869 - -0.1 2,399,880 + -2.2 2,399,940 - +0.7 2,399,960 + -0.4 2,399,977 + -1.1 2,399,985 + -1.1 2,399,993 - +0.3 2,400,030 + -2.2 2,400,040 - -1.8 2,400,082 + -0.2 2,400,094 - -0.2 2,400,099 - opcA Synpcc7942_2333 0.10 -1.7 2,400,104 - opcA Synpcc7942_2333 0.11 -1.5 2,400,127 - opcA Synpcc7942_2333 0.12 -2.1 2,400,210 + opcA Synpcc7942_2333 0.19 -0.3 2,400,223 + opcA Synpcc7942_2333 0.20 -2.4 2,400,223 + opcA Synpcc7942_2333 0.20 -1.9 2,400,231 - -2.6 2,400,231 - opcA Synpcc7942_2333 0.20 -1.4 2,400,269 + opcA Synpcc7942_2333 0.23 -1.4 2,400,277 - opcA Synpcc7942_2333 0.24 -1.7 2,400,296 - opcA Synpcc7942_2333 0.25 -3.1 2,400,316 + opcA Synpcc7942_2333 0.26 -1.2 2,400,343 + opcA Synpcc7942_2333 0.28 -1.2 2,400,388 + opcA Synpcc7942_2333 0.32 -2.9 2,400,396 - opcA Synpcc7942_2333 0.32 -2.0 2,400,448 - opcA Synpcc7942_2333 0.36 -3.4 2,400,458 - opcA Synpcc7942_2333 0.37 -2.1 2,400,500 - opcA Synpcc7942_2333 0.40 -2.6 2,400,576 - opcA Synpcc7942_2333 0.46 -2.4 2,400,586 - opcA Synpcc7942_2333 0.47 -0.4 2,400,691 + opcA Synpcc7942_2333 0.54 -0.9 2,400,699 - opcA Synpcc7942_2333 0.55 -1.3 2,400,713 - opcA Synpcc7942_2333 0.56 -1.8 2,400,714 - opcA Synpcc7942_2333 0.56 -2.3 2,400,763 + opcA Synpcc7942_2333 0.60 -1.1 2,400,789 - opcA Synpcc7942_2333 0.62 -0.8 2,400,789 - opcA Synpcc7942_2333 0.62 -1.0 2,400,797 - opcA Synpcc7942_2333 0.62 -2.4 2,400,808 + opcA Synpcc7942_2333 0.63 -2.2 2,400,810 + opcA Synpcc7942_2333 0.63 -2.0 2,400,851 + opcA Synpcc7942_2333 0.66 -2.4 2,400,853 + opcA Synpcc7942_2333 0.67 -1.1 2,400,858 + opcA Synpcc7942_2333 0.67 -1.9 2,400,866 - opcA Synpcc7942_2333 0.68 -1.0 2,400,908 + opcA Synpcc7942_2333 0.71 -1.8 2,400,908 + opcA Synpcc7942_2333 0.71 -1.8 2,400,908 - opcA Synpcc7942_2333 0.71 -0.2 2,400,915 + opcA Synpcc7942_2333 0.71 -2.7 2,400,915 + opcA Synpcc7942_2333 0.71 -2.0 2,400,923 - opcA Synpcc7942_2333 0.72 +1.0 2,400,933 - opcA Synpcc7942_2333 0.73 -3.8 2,400,938 - opcA Synpcc7942_2333 0.73 -1.1 2,400,938 - opcA Synpcc7942_2333 0.73 -3.1 2,400,948 - opcA Synpcc7942_2333 0.74 -2.1 2,400,958 + opcA Synpcc7942_2333 0.74 -1.9 2,400,963 - opcA Synpcc7942_2333 0.75 -0.3 2,400,966 - opcA Synpcc7942_2333 0.75 -1.1 2,400,966 - opcA Synpcc7942_2333 0.75 -3.6 2,400,976 - opcA Synpcc7942_2333 0.76 -2.5 2,400,986 - opcA Synpcc7942_2333 0.77 -1.0 2,400,995 - opcA Synpcc7942_2333 0.77 -3.2 2,401,016 + opcA Synpcc7942_2333 0.79 -0.7 2,401,016 + opcA Synpcc7942_2333 0.79 -1.6 2,401,016 + opcA Synpcc7942_2333 0.79 -2.1 2,401,022 - opcA Synpcc7942_2333 0.79 -1.0 2,401,024 - opcA Synpcc7942_2333 0.79 +1.9 2,401,024 - opcA Synpcc7942_2333 0.79 -2.6 2,401,090 - opcA Synpcc7942_2333 0.84 -2.5 2,401,102 - opcA Synpcc7942_2333 0.85 -2.4 2,401,107 - opcA Synpcc7942_2333 0.86 -1.8 2,401,113 - opcA Synpcc7942_2333 0.86 -1.6 2,401,139 + opcA Synpcc7942_2333 0.88 -1.1 2,401,145 - opcA Synpcc7942_2333 0.88 -2.3 2,401,168 + -3.1 2,401,180 - -0.7 2,401,190 + -1.8 2,401,201 - -1.9 2,401,211 + -0.5 2,401,211 + -1.1 2,401,211 + +1.0 2,401,211 + +0.0 2,401,214 - -2.4 2,401,219 - -0.9 2,401,219 - -0.1 2,401,219 - -2.8 2,401,381 - -2.6 2,401,492 + -2.9 2,401,500 - zwf Synpcc7942_2334 0.10 -3.6 2,401,507 + zwf Synpcc7942_2334 0.11 -2.0 2,401,507 + zwf Synpcc7942_2334 0.11 -4.8 2,401,507 + zwf Synpcc7942_2334 0.11 -2.7 2,401,515 - zwf Synpcc7942_2334 0.11 -1.8 2,401,515 - zwf Synpcc7942_2334 0.11 -1.5 2,401,515 - zwf Synpcc7942_2334 0.11 -3.2 2,401,537 + zwf Synpcc7942_2334 0.13 -2.4 2,401,540 - zwf Synpcc7942_2334 0.13 -2.3 2,401,545 - zwf Synpcc7942_2334 0.13 -0.4 2,401,552 + zwf Synpcc7942_2334 0.14 -3.2 2,401,553 + zwf Synpcc7942_2334 0.14 -3.5 2,401,568 - zwf Synpcc7942_2334 0.15 -3.3 2,401,584 - zwf Synpcc7942_2334 0.16 -3.8 2,401,602 - zwf Synpcc7942_2334 0.17 -1.0 2,401,607 - zwf Synpcc7942_2334 0.17 -3.2 2,401,609 - zwf Synpcc7942_2334 0.18 -1.3 2,401,631 - zwf Synpcc7942_2334 0.19 -3.3 2,401,654 + zwf Synpcc7942_2334 0.21 -2.6 2,401,665 + zwf Synpcc7942_2334 0.21 -2.4 2,401,667 + zwf Synpcc7942_2334 0.21 -3.5 2,401,667 + zwf Synpcc7942_2334 0.21 -2.3 2,401,673 - zwf Synpcc7942_2334 0.22 -2.7 2,401,691 + zwf Synpcc7942_2334 0.23 -4.8 2,401,697 - zwf Synpcc7942_2334 0.23 -2.9 2,401,697 - zwf Synpcc7942_2334 0.23 -2.6 2,401,698 + zwf Synpcc7942_2334 0.23 -4.5 2,401,698 - zwf Synpcc7942_2334 0.23 -4.0 2,401,706 - zwf Synpcc7942_2334 0.24 -1.6 2,401,751 + zwf Synpcc7942_2334 0.27 -1.8 2,401,761 + zwf Synpcc7942_2334 0.27 -2.5 2,401,779 - zwf Synpcc7942_2334 0.29 -2.4 2,401,792 + zwf Synpcc7942_2334 0.29 -3.0 2,401,793 + zwf Synpcc7942_2334 0.30 -1.8 2,401,800 - zwf Synpcc7942_2334 0.30 -2.7 2,401,803 + zwf Synpcc7942_2334 0.30 -4.9 2,401,803 + zwf Synpcc7942_2334 0.30 -3.4 2,401,829 - zwf Synpcc7942_2334 0.32 -2.2 2,401,829 - zwf Synpcc7942_2334 0.32 -1.6 2,401,837 + zwf Synpcc7942_2334 0.32 -2.9 2,401,861 + zwf Synpcc7942_2334 0.34 -2.3 2,401,872 + zwf Synpcc7942_2334 0.35 -3.5 2,401,879 + zwf Synpcc7942_2334 0.35 -1.3 2,401,879 - zwf Synpcc7942_2334 0.35 -4.2 2,401,890 - zwf Synpcc7942_2334 0.36 -3.4 2,401,905 - zwf Synpcc7942_2334 0.37 -4.1 2,401,920 - zwf Synpcc7942_2334 0.38 -2.8 2,401,943 + zwf Synpcc7942_2334 0.39 -1.6 2,401,943 + zwf Synpcc7942_2334 0.39 -1.7 2,401,943 + zwf Synpcc7942_2334 0.39 -2.2 2,401,943 + zwf Synpcc7942_2334 0.39 -3.2 2,401,943 + zwf Synpcc7942_2334 0.39 -1.5 2,401,943 + zwf Synpcc7942_2334 0.39 -1.4 2,401,943 + zwf Synpcc7942_2334 0.39 -2.3 2,401,943 + zwf Synpcc7942_2334 0.39 -2.4 2,401,951 - zwf Synpcc7942_2334 0.40 -3.5 2,401,951 - zwf Synpcc7942_2334 0.40 -2.3 2,401,951 - zwf Synpcc7942_2334 0.40 +0.4 2,401,956 - zwf Synpcc7942_2334 0.40 -1.0 2,402,010 - zwf Synpcc7942_2334 0.44 -1.8 2,402,012 + zwf Synpcc7942_2334 0.44 -2.2 2,402,012 + zwf Synpcc7942_2334 0.44 -3.4 2,402,020 - zwf Synpcc7942_2334 0.44 -2.8 2,402,020 - zwf Synpcc7942_2334 0.44 -2.4 2,402,020 - zwf Synpcc7942_2334 0.44 -2.1 2,402,051 + zwf Synpcc7942_2334 0.46 -3.0 2,402,051 - zwf Synpcc7942_2334 0.46 -3.2 2,402,053 - zwf Synpcc7942_2334 0.46 -2.0 2,402,053 - zwf Synpcc7942_2334 0.46 -2.4 2,402,083 + zwf Synpcc7942_2334 0.48 -3.2 2,402,086 - zwf Synpcc7942_2334 0.49 -3.9 2,402,098 + zwf Synpcc7942_2334 0.49 -1.6 2,402,103 + zwf Synpcc7942_2334 0.50 -3.6 2,402,106 + zwf Synpcc7942_2334 0.50 -5.4 2,402,107 - zwf Synpcc7942_2334 0.50 -3.1 2,402,110 + zwf Synpcc7942_2334 0.50 -2.2 2,402,142 - zwf Synpcc7942_2334 0.52 -1.2 2,402,173 - zwf Synpcc7942_2334 0.54 -2.9 2,402,173 - zwf Synpcc7942_2334 0.54 -2.1 2,402,179 + zwf Synpcc7942_2334 0.55 -3.6 2,402,182 - zwf Synpcc7942_2334 0.55 -4.8 2,402,187 - zwf Synpcc7942_2334 0.55 -2.2 2,402,198 - zwf Synpcc7942_2334 0.56 -3.1 2,402,203 - zwf Synpcc7942_2334 0.56 -1.6 2,402,217 + zwf Synpcc7942_2334 0.57 -2.9 2,402,278 + zwf Synpcc7942_2334 0.61 -2.7 2,402,284 - zwf Synpcc7942_2334 0.62 -1.3 2,402,284 - zwf Synpcc7942_2334 0.62 -3.2 2,402,285 - zwf Synpcc7942_2334 0.62 -0.6 2,402,299 - zwf Synpcc7942_2334 0.62 -3.5
Or see this region's nucleotide sequence