Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recF and tRNA-Arg are separated by 70 nucleotides tRNA-Arg and Synpcc7942_2251 overlap by 47 nucleotides Synpcc7942_2251 and ppc are separated by 7 nucleotides
Synpcc7942_2250: recF - recombination protein F, at 2,317,075 to 2,318,238
recF
Synpcc7942_R0010: tRNA-Arg - tRNA-Arg, at 2,318,309 to 2,318,381
tRNA-Arg
Synpcc7942_2251: Synpcc7942_2251 - hypothetical protein, at 2,318,335 to 2,318,919
_2251
Synpcc7942_2252: ppc - phosphoenolpyruvate carboxylase, at 2,318,927 to 2,321,980
ppc
Position (kb)
2318
2319 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2317.365 kb on + strand, within recF at 2317.428 kb on + strand, within recF at 2317.538 kb on + strand, within recF at 2317.552 kb on + strand, within recF at 2317.639 kb on + strand, within recF at 2317.724 kb on - strand, within recF at 2317.751 kb on + strand, within recF at 2317.821 kb on + strand, within recF at 2318.096 kb on + strand, within recF at 2318.217 kb on + strand at 2318.225 kb on - strand at 2318.259 kb on + strand at 2318.267 kb on - strand at 2318.269 kb on + strand at 2318.290 kb on - strand at 2318.290 kb on - strand at 2318.290 kb on - strand at 2318.383 kb on - strand at 2318.420 kb on - strand, within Synpcc7942_2251 at 2318.477 kb on + strand, within Synpcc7942_2251 at 2318.477 kb on + strand, within Synpcc7942_2251 at 2318.485 kb on - strand, within Synpcc7942_2251 at 2318.485 kb on - strand, within Synpcc7942_2251 at 2318.485 kb on - strand, within Synpcc7942_2251 at 2318.485 kb on - strand, within Synpcc7942_2251 at 2318.485 kb on - strand, within Synpcc7942_2251 at 2318.485 kb on - strand, within Synpcc7942_2251 at 2318.485 kb on - strand, within Synpcc7942_2251 at 2318.518 kb on + strand, within Synpcc7942_2251 at 2318.560 kb on + strand, within Synpcc7942_2251 at 2318.560 kb on + strand, within Synpcc7942_2251 at 2318.560 kb on - strand, within Synpcc7942_2251 at 2318.568 kb on - strand, within Synpcc7942_2251 at 2318.568 kb on - strand, within Synpcc7942_2251 at 2318.590 kb on + strand, within Synpcc7942_2251 at 2318.598 kb on - strand, within Synpcc7942_2251 at 2318.604 kb on + strand, within Synpcc7942_2251 at 2318.628 kb on - strand, within Synpcc7942_2251 at 2318.653 kb on - strand, within Synpcc7942_2251 at 2318.667 kb on - strand, within Synpcc7942_2251 at 2318.675 kb on - strand, within Synpcc7942_2251 at 2318.691 kb on + strand, within Synpcc7942_2251 at 2318.699 kb on + strand, within Synpcc7942_2251 at 2318.699 kb on - strand, within Synpcc7942_2251 at 2318.716 kb on + strand, within Synpcc7942_2251 at 2318.765 kb on + strand, within Synpcc7942_2251 at 2318.772 kb on - strand, within Synpcc7942_2251 at 2318.773 kb on - strand, within Synpcc7942_2251 at 2318.778 kb on - strand, within Synpcc7942_2251 at 2318.813 kb on + strand at 2318.854 kb on - strand, within Synpcc7942_2251 at 2318.870 kb on - strand at 2318.932 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 2,317,365 + recF Synpcc7942_2250 0.25 -1.0 2,317,428 + recF Synpcc7942_2250 0.30 +0.5 2,317,538 + recF Synpcc7942_2250 0.40 -1.0 2,317,552 + recF Synpcc7942_2250 0.41 -0.6 2,317,639 + recF Synpcc7942_2250 0.48 +0.6 2,317,724 - recF Synpcc7942_2250 0.56 -1.4 2,317,751 + recF Synpcc7942_2250 0.58 -0.6 2,317,821 + recF Synpcc7942_2250 0.64 +1.2 2,318,096 + recF Synpcc7942_2250 0.88 +1.5 2,318,217 + +1.2 2,318,225 - -2.1 2,318,259 + -1.1 2,318,267 - +0.1 2,318,269 + -0.4 2,318,290 - -0.0 2,318,290 - +0.0 2,318,290 - +1.8 2,318,383 - -1.9 2,318,420 - Synpcc7942_2251 0.15 -0.9 2,318,477 + Synpcc7942_2251 0.24 -0.8 2,318,477 + Synpcc7942_2251 0.24 -0.0 2,318,485 - Synpcc7942_2251 0.26 +0.4 2,318,485 - Synpcc7942_2251 0.26 -0.8 2,318,485 - Synpcc7942_2251 0.26 -0.3 2,318,485 - Synpcc7942_2251 0.26 -0.4 2,318,485 - Synpcc7942_2251 0.26 -1.0 2,318,485 - Synpcc7942_2251 0.26 -0.0 2,318,485 - Synpcc7942_2251 0.26 +0.5 2,318,518 + Synpcc7942_2251 0.31 -0.9 2,318,560 + Synpcc7942_2251 0.38 -1.7 2,318,560 + Synpcc7942_2251 0.38 -1.3 2,318,560 - Synpcc7942_2251 0.38 -1.1 2,318,568 - Synpcc7942_2251 0.40 -1.6 2,318,568 - Synpcc7942_2251 0.40 -0.8 2,318,590 + Synpcc7942_2251 0.44 -2.4 2,318,598 - Synpcc7942_2251 0.45 -3.0 2,318,604 + Synpcc7942_2251 0.46 -1.6 2,318,628 - Synpcc7942_2251 0.50 -1.9 2,318,653 - Synpcc7942_2251 0.54 -1.0 2,318,667 - Synpcc7942_2251 0.57 -1.6 2,318,675 - Synpcc7942_2251 0.58 -0.3 2,318,691 + Synpcc7942_2251 0.61 -0.8 2,318,699 + Synpcc7942_2251 0.62 -1.0 2,318,699 - Synpcc7942_2251 0.62 -0.6 2,318,716 + Synpcc7942_2251 0.65 -2.7 2,318,765 + Synpcc7942_2251 0.74 -0.3 2,318,772 - Synpcc7942_2251 0.75 -1.3 2,318,773 - Synpcc7942_2251 0.75 -1.3 2,318,778 - Synpcc7942_2251 0.76 -1.2 2,318,813 + -1.0 2,318,854 - Synpcc7942_2251 0.89 -1.5 2,318,870 - -1.4 2,318,932 - +0.6
Or see this region's nucleotide sequence