Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_2181 and Synpcc7942_2182 are separated by 52 nucleotides Synpcc7942_2182 and rnhA are separated by 126 nucleotides
Synpcc7942_2181: Synpcc7942_2181 - hypothetical protein, at 2,259,980 to 2,261,191
_2181
Synpcc7942_2182: Synpcc7942_2182 - hypothetical protein, at 2,261,244 to 2,261,429
_2182
Synpcc7942_2183: rnhA - RNase HI, at 2,261,556 to 2,262,335
rnhA
Position (kb)
2261
2262 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2260.257 kb on - strand, within Synpcc7942_2181 at 2260.410 kb on - strand, within Synpcc7942_2181 at 2260.426 kb on + strand, within Synpcc7942_2181 at 2260.488 kb on - strand, within Synpcc7942_2181 at 2260.546 kb on + strand, within Synpcc7942_2181 at 2260.624 kb on + strand, within Synpcc7942_2181 at 2260.646 kb on - strand, within Synpcc7942_2181 at 2260.648 kb on - strand, within Synpcc7942_2181 at 2260.674 kb on + strand, within Synpcc7942_2181 at 2260.696 kb on + strand, within Synpcc7942_2181 at 2260.696 kb on + strand, within Synpcc7942_2181 at 2260.796 kb on - strand, within Synpcc7942_2181 at 2260.827 kb on + strand, within Synpcc7942_2181 at 2260.845 kb on - strand, within Synpcc7942_2181 at 2260.980 kb on - strand, within Synpcc7942_2181 at 2260.993 kb on - strand, within Synpcc7942_2181 at 2260.997 kb on + strand, within Synpcc7942_2181 at 2260.997 kb on + strand, within Synpcc7942_2181 at 2261.005 kb on - strand, within Synpcc7942_2181 at 2261.024 kb on + strand, within Synpcc7942_2181 at 2261.051 kb on - strand, within Synpcc7942_2181 at 2261.051 kb on - strand, within Synpcc7942_2181 at 2261.096 kb on - strand at 2261.145 kb on - strand at 2261.164 kb on - strand at 2261.183 kb on + strand at 2261.187 kb on + strand at 2261.194 kb on + strand at 2261.256 kb on + strand at 2261.341 kb on + strand, within Synpcc7942_2182 at 2261.348 kb on + strand, within Synpcc7942_2182 at 2261.356 kb on - strand, within Synpcc7942_2182 at 2261.431 kb on + strand at 2261.431 kb on + strand at 2261.433 kb on + strand at 2261.519 kb on + strand at 2261.527 kb on - strand at 2261.527 kb on - strand at 2261.537 kb on - strand at 2261.553 kb on + strand at 2261.555 kb on + strand at 2261.561 kb on - strand at 2261.593 kb on + strand at 2261.612 kb on + strand at 2261.615 kb on + strand at 2261.667 kb on - strand, within rnhA at 2261.674 kb on + strand, within rnhA at 2261.675 kb on + strand, within rnhA at 2261.681 kb on + strand, within rnhA at 2261.698 kb on - strand, within rnhA at 2261.698 kb on - strand, within rnhA at 2261.756 kb on - strand, within rnhA at 2261.773 kb on - strand, within rnhA at 2261.785 kb on + strand, within rnhA at 2261.786 kb on + strand, within rnhA at 2261.791 kb on + strand, within rnhA at 2261.798 kb on + strand, within rnhA at 2261.801 kb on - strand, within rnhA at 2261.801 kb on - strand, within rnhA at 2261.810 kb on + strand, within rnhA at 2261.846 kb on + strand, within rnhA at 2261.908 kb on - strand, within rnhA at 2261.970 kb on + strand, within rnhA at 2261.970 kb on + strand, within rnhA at 2261.978 kb on - strand, within rnhA at 2261.983 kb on + strand, within rnhA at 2261.996 kb on + strand, within rnhA at 2262.024 kb on + strand, within rnhA at 2262.031 kb on + strand, within rnhA at 2262.031 kb on + strand, within rnhA at 2262.063 kb on - strand, within rnhA at 2262.071 kb on + strand, within rnhA at 2262.071 kb on - strand, within rnhA at 2262.077 kb on + strand, within rnhA at 2262.077 kb on + strand, within rnhA at 2262.085 kb on - strand, within rnhA at 2262.085 kb on - strand, within rnhA at 2262.088 kb on - strand, within rnhA at 2262.091 kb on + strand, within rnhA at 2262.115 kb on + strand, within rnhA at 2262.123 kb on - strand, within rnhA at 2262.123 kb on - strand, within rnhA at 2262.125 kb on - strand, within rnhA at 2262.140 kb on - strand, within rnhA at 2262.159 kb on + strand, within rnhA at 2262.195 kb on + strand, within rnhA at 2262.211 kb on + strand, within rnhA at 2262.225 kb on - strand, within rnhA at 2262.263 kb on + strand at 2262.263 kb on + strand at 2262.278 kb on + strand at 2262.278 kb on + strand at 2262.278 kb on + strand at 2262.278 kb on + strand at 2262.278 kb on + strand at 2262.278 kb on + strand at 2262.278 kb on + strand at 2262.278 kb on + strand at 2262.278 kb on + strand at 2262.278 kb on + strand at 2262.278 kb on + strand at 2262.283 kb on + strand at 2262.286 kb on - strand at 2262.286 kb on - strand at 2262.286 kb on - strand at 2262.286 kb on - strand at 2262.286 kb on - strand at 2262.307 kb on + strand at 2262.307 kb on + strand at 2262.334 kb on - strand at 2262.353 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 2,260,257 - Synpcc7942_2181 0.23 -1.6 2,260,410 - Synpcc7942_2181 0.35 -1.5 2,260,426 + Synpcc7942_2181 0.37 -2.8 2,260,488 - Synpcc7942_2181 0.42 -2.0 2,260,546 + Synpcc7942_2181 0.47 -1.6 2,260,624 + Synpcc7942_2181 0.53 -0.6 2,260,646 - Synpcc7942_2181 0.55 -3.3 2,260,648 - Synpcc7942_2181 0.55 +2.7 2,260,674 + Synpcc7942_2181 0.57 -2.2 2,260,696 + Synpcc7942_2181 0.59 +0.5 2,260,696 + Synpcc7942_2181 0.59 -0.6 2,260,796 - Synpcc7942_2181 0.67 -2.0 2,260,827 + Synpcc7942_2181 0.70 -2.2 2,260,845 - Synpcc7942_2181 0.71 -2.8 2,260,980 - Synpcc7942_2181 0.83 -3.0 2,260,993 - Synpcc7942_2181 0.84 -2.0 2,260,997 + Synpcc7942_2181 0.84 -2.1 2,260,997 + Synpcc7942_2181 0.84 -2.8 2,261,005 - Synpcc7942_2181 0.85 -1.0 2,261,024 + Synpcc7942_2181 0.86 -0.8 2,261,051 - Synpcc7942_2181 0.88 -1.6 2,261,051 - Synpcc7942_2181 0.88 -2.5 2,261,096 - -0.6 2,261,145 - -2.7 2,261,164 - -2.2 2,261,183 + +0.4 2,261,187 + +0.5 2,261,194 + +1.8 2,261,256 + +0.5 2,261,341 + Synpcc7942_2182 0.52 +1.1 2,261,348 + Synpcc7942_2182 0.56 +0.6 2,261,356 - Synpcc7942_2182 0.60 -0.2 2,261,431 + +0.1 2,261,431 + -0.2 2,261,433 + +0.3 2,261,519 + +0.5 2,261,527 - +1.4 2,261,527 - +1.6 2,261,537 - +1.0 2,261,553 + +0.5 2,261,555 + +0.6 2,261,561 - +0.3 2,261,593 + +1.0 2,261,612 + +2.2 2,261,615 + +2.2 2,261,667 - rnhA Synpcc7942_2183 0.14 +1.4 2,261,674 + rnhA Synpcc7942_2183 0.15 +1.2 2,261,675 + rnhA Synpcc7942_2183 0.15 +3.0 2,261,681 + rnhA Synpcc7942_2183 0.16 +2.0 2,261,698 - rnhA Synpcc7942_2183 0.18 +1.9 2,261,698 - rnhA Synpcc7942_2183 0.18 +1.8 2,261,756 - rnhA Synpcc7942_2183 0.26 +1.6 2,261,773 - rnhA Synpcc7942_2183 0.28 +0.4 2,261,785 + rnhA Synpcc7942_2183 0.29 +0.8 2,261,786 + rnhA Synpcc7942_2183 0.29 +0.4 2,261,791 + rnhA Synpcc7942_2183 0.30 +1.3 2,261,798 + rnhA Synpcc7942_2183 0.31 +2.3 2,261,801 - rnhA Synpcc7942_2183 0.31 +0.5 2,261,801 - rnhA Synpcc7942_2183 0.31 +0.9 2,261,810 + rnhA Synpcc7942_2183 0.33 +1.3 2,261,846 + rnhA Synpcc7942_2183 0.37 +1.1 2,261,908 - rnhA Synpcc7942_2183 0.45 +0.5 2,261,970 + rnhA Synpcc7942_2183 0.53 +0.2 2,261,970 + rnhA Synpcc7942_2183 0.53 -0.0 2,261,978 - rnhA Synpcc7942_2183 0.54 -0.3 2,261,983 + rnhA Synpcc7942_2183 0.55 +0.7 2,261,996 + rnhA Synpcc7942_2183 0.56 -0.4 2,262,024 + rnhA Synpcc7942_2183 0.60 -0.0 2,262,031 + rnhA Synpcc7942_2183 0.61 -1.3 2,262,031 + rnhA Synpcc7942_2183 0.61 -0.2 2,262,063 - rnhA Synpcc7942_2183 0.65 +0.2 2,262,071 + rnhA Synpcc7942_2183 0.66 -0.1 2,262,071 - rnhA Synpcc7942_2183 0.66 -0.1 2,262,077 + rnhA Synpcc7942_2183 0.67 +0.5 2,262,077 + rnhA Synpcc7942_2183 0.67 -1.7 2,262,085 - rnhA Synpcc7942_2183 0.68 -1.3 2,262,085 - rnhA Synpcc7942_2183 0.68 -0.3 2,262,088 - rnhA Synpcc7942_2183 0.68 -3.3 2,262,091 + rnhA Synpcc7942_2183 0.69 -0.8 2,262,115 + rnhA Synpcc7942_2183 0.72 +0.4 2,262,123 - rnhA Synpcc7942_2183 0.73 +0.0 2,262,123 - rnhA Synpcc7942_2183 0.73 -0.0 2,262,125 - rnhA Synpcc7942_2183 0.73 -0.1 2,262,140 - rnhA Synpcc7942_2183 0.75 -0.0 2,262,159 + rnhA Synpcc7942_2183 0.77 -0.0 2,262,195 + rnhA Synpcc7942_2183 0.82 -0.9 2,262,211 + rnhA Synpcc7942_2183 0.84 +0.3 2,262,225 - rnhA Synpcc7942_2183 0.86 +1.8 2,262,263 + +0.8 2,262,263 + -0.2 2,262,278 + -0.0 2,262,278 + -0.1 2,262,278 + -1.8 2,262,278 + -3.0 2,262,278 + +0.1 2,262,278 + +0.3 2,262,278 + -0.0 2,262,278 + -1.2 2,262,278 + -1.5 2,262,278 + -0.5 2,262,278 + -0.2 2,262,283 + -0.9 2,262,286 - +1.0 2,262,286 - -0.1 2,262,286 - -0.2 2,262,286 - -0.1 2,262,286 - +0.4 2,262,307 + -1.1 2,262,307 + +0.5 2,262,334 - -0.0 2,262,353 - +0.1
Or see this region's nucleotide sequence