Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt psbX and Synpcc7942_2017 are separated by 154 nucleotides Synpcc7942_2017 and tRNA-Thr are separated by 81 nucleotides tRNA-Thr and Synpcc7942_2018 are separated by 60 nucleotides Synpcc7942_2018 and Synpcc7942_2019 are separated by 63 nucleotides Synpcc7942_2019 and infB are separated by 82 nucleotides
Synpcc7942_2016: psbX - photosystem II PsbX protein, at 2,084,803 to 2,084,922
psbX
Synpcc7942_2017: Synpcc7942_2017 - hypothetical protein, at 2,085,077 to 2,085,370
_2017
Synpcc7942_R0003: tRNA-Thr - tRNA-Thr, at 2,085,452 to 2,085,523
tRNA-Thr
Synpcc7942_2018: Synpcc7942_2018 - hypothetical protein, at 2,085,584 to 2,085,829
_2018
Synpcc7942_2019: Synpcc7942_2019 - hypothetical protein, at 2,085,893 to 2,086,762
_2019
Synpcc7942_2020: infB - translation initiation factor IF-2, at 2,086,845 to 2,089,937
infB
Position (kb)
2085
2086
2087 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2084.949 kb on - strand at 2084.964 kb on + strand at 2084.972 kb on - strand at 2084.972 kb on - strand at 2085.002 kb on + strand at 2085.010 kb on - strand at 2085.014 kb on + strand at 2085.046 kb on + strand at 2085.053 kb on - strand at 2085.413 kb on - strand at 2085.564 kb on - strand at 2085.616 kb on + strand, within Synpcc7942_2018 at 2085.630 kb on - strand, within Synpcc7942_2018 at 2085.741 kb on + strand, within Synpcc7942_2018 at 2085.751 kb on + strand, within Synpcc7942_2018 at 2085.784 kb on - strand, within Synpcc7942_2018 at 2085.825 kb on - strand at 2085.831 kb on + strand at 2085.839 kb on - strand at 2085.852 kb on - strand at 2085.883 kb on + strand at 2085.885 kb on - strand at 2085.899 kb on - strand at 2085.907 kb on + strand at 2085.913 kb on - strand at 2085.913 kb on - strand at 2086.026 kb on + strand, within Synpcc7942_2019 at 2086.026 kb on + strand at 2086.155 kb on - strand, within Synpcc7942_2019 at 2086.184 kb on + strand, within Synpcc7942_2019 at 2086.184 kb on + strand, within Synpcc7942_2019 at 2086.192 kb on - strand, within Synpcc7942_2019 at 2086.231 kb on - strand, within Synpcc7942_2019 at 2086.252 kb on - strand, within Synpcc7942_2019 at 2086.273 kb on + strand, within Synpcc7942_2019 at 2086.273 kb on + strand, within Synpcc7942_2019 at 2086.301 kb on + strand, within Synpcc7942_2019 at 2086.438 kb on + strand, within Synpcc7942_2019 at 2086.517 kb on - strand, within Synpcc7942_2019 at 2086.527 kb on + strand, within Synpcc7942_2019 at 2086.585 kb on - strand, within Synpcc7942_2019 at 2086.590 kb on - strand, within Synpcc7942_2019 at 2086.594 kb on + strand, within Synpcc7942_2019 at 2086.697 kb on - strand at 2086.775 kb on + strand at 2086.783 kb on - strand at 2086.840 kb on + strand at 2086.842 kb on + strand at 2086.882 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 2,084,949 - +0.2 2,084,964 + -1.3 2,084,972 - +0.7 2,084,972 - -0.3 2,085,002 + -0.1 2,085,010 - -0.4 2,085,014 + -0.4 2,085,046 + +0.6 2,085,053 - -0.6 2,085,413 - -0.6 2,085,564 - -1.6 2,085,616 + Synpcc7942_2018 0.13 -1.1 2,085,630 - Synpcc7942_2018 0.19 -0.2 2,085,741 + Synpcc7942_2018 0.64 +1.3 2,085,751 + Synpcc7942_2018 0.68 +1.1 2,085,784 - Synpcc7942_2018 0.81 +1.0 2,085,825 - -0.1 2,085,831 + +0.4 2,085,839 - +0.1 2,085,852 - -0.9 2,085,883 + +0.2 2,085,885 - -0.2 2,085,899 - +0.6 2,085,907 + -0.4 2,085,913 - -0.5 2,085,913 - -0.6 2,086,026 + Synpcc7942_2019 0.15 -1.4 2,086,026 + -1.5 2,086,155 - Synpcc7942_2019 0.30 +0.4 2,086,184 + Synpcc7942_2019 0.33 -2.1 2,086,184 + Synpcc7942_2019 0.33 -1.6 2,086,192 - Synpcc7942_2019 0.34 -1.6 2,086,231 - Synpcc7942_2019 0.39 -1.7 2,086,252 - Synpcc7942_2019 0.41 -2.4 2,086,273 + Synpcc7942_2019 0.44 -2.2 2,086,273 + Synpcc7942_2019 0.44 -3.1 2,086,301 + Synpcc7942_2019 0.47 -2.3 2,086,438 + Synpcc7942_2019 0.63 -1.2 2,086,517 - Synpcc7942_2019 0.72 -0.9 2,086,527 + Synpcc7942_2019 0.73 -2.0 2,086,585 - Synpcc7942_2019 0.80 -1.6 2,086,590 - Synpcc7942_2019 0.80 +0.5 2,086,594 + Synpcc7942_2019 0.81 -2.7 2,086,697 - -2.1 2,086,775 + -0.3 2,086,783 - +0.2 2,086,840 + -0.6 2,086,842 + -0.3 2,086,882 - +0.5
Or see this region's nucleotide sequence