Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1555 and Synpcc7942_1556 are separated by 121 nucleotides Synpcc7942_1556 and rpoD6 are separated by 15 nucleotides rpoD6 and Synpcc7942_1558 are separated by 807 nucleotides
Synpcc7942_1555: Synpcc7942_1555 - Thf1-like protein, at 1,611,906 to 1,612,748
_1555
Synpcc7942_1556: Synpcc7942_1556 - hypothetical protein, at 1,612,870 to 1,613,448
_1556
Synpcc7942_1557: rpoD6 - principal RNA polymerase sigma factor SigA, at 1,613,464 to 1,614,396
rpoD6
Synpcc7942_1558: Synpcc7942_1558 - hypothetical protein, at 1,615,204 to 1,616,271
_1558
Position (kb)
1613
1614
1615 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1612.475 kb on + strand, within Synpcc7942_1555 at 1612.483 kb on - strand, within Synpcc7942_1555 at 1612.600 kb on + strand, within Synpcc7942_1555 at 1612.628 kb on + strand, within Synpcc7942_1555 at 1612.633 kb on + strand, within Synpcc7942_1555 at 1612.641 kb on - strand, within Synpcc7942_1555 at 1612.717 kb on - strand at 1612.748 kb on - strand at 1612.753 kb on - strand at 1612.757 kb on - strand at 1612.788 kb on + strand at 1612.788 kb on + strand at 1612.788 kb on + strand at 1612.796 kb on - strand at 1612.811 kb on - strand at 1612.840 kb on - strand at 1612.899 kb on - strand at 1612.912 kb on - strand at 1612.916 kb on + strand at 1612.916 kb on + strand at 1612.917 kb on - strand at 1612.921 kb on + strand at 1612.923 kb on - strand at 1612.924 kb on - strand at 1612.926 kb on + strand at 1612.932 kb on + strand, within Synpcc7942_1556 at 1612.934 kb on - strand, within Synpcc7942_1556 at 1612.934 kb on - strand, within Synpcc7942_1556 at 1612.935 kb on - strand, within Synpcc7942_1556 at 1612.940 kb on - strand, within Synpcc7942_1556 at 1612.950 kb on - strand, within Synpcc7942_1556 at 1612.957 kb on - strand, within Synpcc7942_1556 at 1613.004 kb on + strand, within Synpcc7942_1556 at 1613.057 kb on + strand, within Synpcc7942_1556 at 1613.067 kb on + strand, within Synpcc7942_1556 at 1613.070 kb on - strand, within Synpcc7942_1556 at 1613.075 kb on - strand, within Synpcc7942_1556 at 1613.079 kb on + strand, within Synpcc7942_1556 at 1613.124 kb on + strand, within Synpcc7942_1556 at 1613.127 kb on + strand, within Synpcc7942_1556 at 1613.132 kb on + strand, within Synpcc7942_1556 at 1613.193 kb on - strand, within Synpcc7942_1556 at 1613.193 kb on - strand, within Synpcc7942_1556 at 1613.215 kb on + strand, within Synpcc7942_1556 at 1613.253 kb on + strand at 1613.265 kb on + strand, within Synpcc7942_1556 at 1613.275 kb on - strand, within Synpcc7942_1556 at 1613.376 kb on + strand, within Synpcc7942_1556 at 1613.384 kb on - strand, within Synpcc7942_1556 at 1613.385 kb on - strand, within Synpcc7942_1556 at 1613.399 kb on + strand at 1613.428 kb on + strand at 1613.455 kb on - strand at 1613.455 kb on - strand at 1613.518 kb on + strand at 1613.548 kb on + strand at 1613.577 kb on + strand, within rpoD6 at 1613.578 kb on + strand, within rpoD6 at 1613.586 kb on - strand, within rpoD6 at 1613.586 kb on - strand, within rpoD6 at 1613.586 kb on - strand, within rpoD6 at 1613.604 kb on - strand, within rpoD6 at 1613.637 kb on - strand, within rpoD6 at 1613.657 kb on + strand, within rpoD6 at 1613.657 kb on + strand, within rpoD6 at 1613.754 kb on + strand, within rpoD6 at 1613.762 kb on - strand, within rpoD6 at 1613.780 kb on - strand, within rpoD6 at 1613.818 kb on + strand, within rpoD6 at 1613.830 kb on + strand, within rpoD6 at 1613.943 kb on + strand, within rpoD6 at 1613.951 kb on - strand, within rpoD6 at 1613.961 kb on - strand, within rpoD6 at 1613.981 kb on - strand, within rpoD6 at 1613.996 kb on - strand, within rpoD6 at 1614.026 kb on + strand, within rpoD6 at 1614.104 kb on - strand, within rpoD6 at 1614.112 kb on - strand, within rpoD6 at 1614.165 kb on - strand, within rpoD6 at 1614.283 kb on - strand, within rpoD6 at 1614.315 kb on + strand at 1614.552 kb on - strand at 1614.607 kb on + strand at 1614.607 kb on + strand at 1614.699 kb on + strand at 1614.925 kb on - strand at 1614.928 kb on + strand at 1614.928 kb on + strand at 1614.936 kb on + strand at 1614.936 kb on - strand at 1614.936 kb on - strand at 1614.976 kb on - strand at 1614.997 kb on + strand at 1615.081 kb on + strand at 1615.085 kb on - strand at 1615.085 kb on - strand at 1615.180 kb on + strand at 1615.188 kb on - strand at 1615.188 kb on - strand at 1615.214 kb on - strand at 1615.216 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 1,612,475 + Synpcc7942_1555 0.67 -0.3 1,612,483 - Synpcc7942_1555 0.68 +1.2 1,612,600 + Synpcc7942_1555 0.82 +0.1 1,612,628 + Synpcc7942_1555 0.86 +0.4 1,612,633 + Synpcc7942_1555 0.86 -1.1 1,612,641 - Synpcc7942_1555 0.87 -0.9 1,612,717 - +0.5 1,612,748 - -1.1 1,612,753 - -0.0 1,612,757 - -0.6 1,612,788 + +2.0 1,612,788 + -0.3 1,612,788 + +0.1 1,612,796 - -0.6 1,612,811 - -0.9 1,612,840 - -2.4 1,612,899 - -0.9 1,612,912 - +0.5 1,612,916 + -0.3 1,612,916 + -1.6 1,612,917 - -0.2 1,612,921 + +1.6 1,612,923 - -0.6 1,612,924 - -1.7 1,612,926 + -0.1 1,612,932 + Synpcc7942_1556 0.11 -0.6 1,612,934 - Synpcc7942_1556 0.11 +0.5 1,612,934 - Synpcc7942_1556 0.11 +0.4 1,612,935 - Synpcc7942_1556 0.11 -1.1 1,612,940 - Synpcc7942_1556 0.12 -1.0 1,612,950 - Synpcc7942_1556 0.14 +0.4 1,612,957 - Synpcc7942_1556 0.15 +0.2 1,613,004 + Synpcc7942_1556 0.23 +0.8 1,613,057 + Synpcc7942_1556 0.32 -1.6 1,613,067 + Synpcc7942_1556 0.34 +0.4 1,613,070 - Synpcc7942_1556 0.35 -1.8 1,613,075 - Synpcc7942_1556 0.35 -0.6 1,613,079 + Synpcc7942_1556 0.36 +0.2 1,613,124 + Synpcc7942_1556 0.44 +0.3 1,613,127 + Synpcc7942_1556 0.44 +0.1 1,613,132 + Synpcc7942_1556 0.45 -1.0 1,613,193 - Synpcc7942_1556 0.56 -0.0 1,613,193 - Synpcc7942_1556 0.56 -0.2 1,613,215 + Synpcc7942_1556 0.60 +0.7 1,613,253 + +0.2 1,613,265 + Synpcc7942_1556 0.68 -1.0 1,613,275 - Synpcc7942_1556 0.70 -0.7 1,613,376 + Synpcc7942_1556 0.87 -1.2 1,613,384 - Synpcc7942_1556 0.89 -0.2 1,613,385 - Synpcc7942_1556 0.89 -0.9 1,613,399 + +0.1 1,613,428 + -0.2 1,613,455 - -1.7 1,613,455 - -0.9 1,613,518 + -1.3 1,613,548 + -0.3 1,613,577 + rpoD6 Synpcc7942_1557 0.12 +0.3 1,613,578 + rpoD6 Synpcc7942_1557 0.12 -1.7 1,613,586 - rpoD6 Synpcc7942_1557 0.13 -1.8 1,613,586 - rpoD6 Synpcc7942_1557 0.13 -1.0 1,613,586 - rpoD6 Synpcc7942_1557 0.13 -0.7 1,613,604 - rpoD6 Synpcc7942_1557 0.15 -2.0 1,613,637 - rpoD6 Synpcc7942_1557 0.19 -0.6 1,613,657 + rpoD6 Synpcc7942_1557 0.21 -0.6 1,613,657 + rpoD6 Synpcc7942_1557 0.21 -1.0 1,613,754 + rpoD6 Synpcc7942_1557 0.31 -1.5 1,613,762 - rpoD6 Synpcc7942_1557 0.32 -0.6 1,613,780 - rpoD6 Synpcc7942_1557 0.34 -2.5 1,613,818 + rpoD6 Synpcc7942_1557 0.38 -3.3 1,613,830 + rpoD6 Synpcc7942_1557 0.39 -1.0 1,613,943 + rpoD6 Synpcc7942_1557 0.51 -2.2 1,613,951 - rpoD6 Synpcc7942_1557 0.52 -2.2 1,613,961 - rpoD6 Synpcc7942_1557 0.53 -2.6 1,613,981 - rpoD6 Synpcc7942_1557 0.55 -3.3 1,613,996 - rpoD6 Synpcc7942_1557 0.57 -2.7 1,614,026 + rpoD6 Synpcc7942_1557 0.60 -1.7 1,614,104 - rpoD6 Synpcc7942_1557 0.69 -3.2 1,614,112 - rpoD6 Synpcc7942_1557 0.69 -0.5 1,614,165 - rpoD6 Synpcc7942_1557 0.75 -2.1 1,614,283 - rpoD6 Synpcc7942_1557 0.88 -0.6 1,614,315 + -4.4 1,614,552 - -1.1 1,614,607 + -0.4 1,614,607 + -0.4 1,614,699 + -1.3 1,614,925 - -0.8 1,614,928 + -1.6 1,614,928 + -0.8 1,614,936 + +0.9 1,614,936 - -0.3 1,614,936 - -0.3 1,614,976 - -0.4 1,614,997 + +0.8 1,615,081 + +0.0 1,615,085 - +1.7 1,615,085 - +0.3 1,615,180 + -0.7 1,615,188 - +0.0 1,615,188 - -0.6 1,615,214 - +0.2 1,615,216 - -0.0
Or see this region's nucleotide sequence