Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt modA and Synpcc7942_1532 overlap by 4 nucleotides Synpcc7942_1532 and sdhB overlap by 23 nucleotides sdhB and Synpcc7942_1534 are separated by 121 nucleotides
Synpcc7942_1531: modA - molybdenum ABC transporter, periplasmic molybdate-binding protein, at 1,590,799 to 1,591,614
modA
Synpcc7942_1532: Synpcc7942_1532 - Molybdate ABC transporter, permease protein, at 1,591,611 to 1,592,276
_1532
Synpcc7942_1533: sdhB - succinate dehydrogenase/fumarate reductase iron-sulfur subunit, at 1,592,254 to 1,593,093
sdhB
Synpcc7942_1534: Synpcc7942_1534 - hypothetical protein, at 1,593,215 to 1,595,422
_1534
Position (kb)
1592
1593
1594 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1591.284 kb on + strand, within modA at 1591.373 kb on - strand, within modA at 1591.462 kb on + strand, within modA at 1591.559 kb on - strand at 1591.628 kb on - strand at 1591.628 kb on - strand at 1592.051 kb on + strand, within Synpcc7942_1532 at 1592.230 kb on - strand at 1592.249 kb on + strand at 1592.249 kb on + strand at 1592.271 kb on + strand at 1592.314 kb on - strand at 1592.327 kb on + strand at 1592.327 kb on + strand at 1592.335 kb on - strand at 1592.342 kb on - strand, within sdhB at 1592.390 kb on + strand, within sdhB at 1592.396 kb on - strand, within sdhB at 1592.398 kb on - strand, within sdhB at 1592.400 kb on + strand, within sdhB at 1592.400 kb on + strand, within sdhB at 1592.408 kb on + strand, within sdhB at 1592.408 kb on - strand, within sdhB at 1592.419 kb on + strand, within sdhB at 1592.445 kb on + strand, within sdhB at 1592.451 kb on - strand, within sdhB at 1592.460 kb on - strand, within sdhB at 1592.485 kb on - strand, within sdhB at 1592.489 kb on + strand, within sdhB at 1592.497 kb on - strand, within sdhB at 1592.551 kb on - strand, within sdhB at 1592.560 kb on + strand, within sdhB at 1592.596 kb on + strand, within sdhB at 1592.602 kb on + strand, within sdhB at 1592.647 kb on - strand, within sdhB at 1592.664 kb on - strand, within sdhB at 1592.664 kb on - strand, within sdhB at 1592.674 kb on + strand, within sdhB at 1592.674 kb on + strand at 1592.681 kb on - strand, within sdhB at 1592.682 kb on - strand, within sdhB at 1592.721 kb on + strand, within sdhB at 1592.728 kb on + strand, within sdhB at 1592.733 kb on - strand, within sdhB at 1592.733 kb on - strand, within sdhB at 1592.795 kb on - strand, within sdhB at 1592.848 kb on - strand, within sdhB at 1592.950 kb on - strand, within sdhB at 1592.961 kb on + strand, within sdhB at 1592.982 kb on - strand, within sdhB at 1592.982 kb on - strand, within sdhB at 1593.037 kb on + strand at 1593.042 kb on + strand at 1593.055 kb on - strand at 1593.096 kb on - strand at 1593.123 kb on + strand at 1593.123 kb on + strand at 1593.140 kb on + strand at 1593.171 kb on + strand at 1593.171 kb on + strand at 1593.179 kb on - strand at 1593.179 kb on - strand at 1593.179 kb on - strand at 1593.179 kb on - strand at 1593.179 kb on - strand at 1593.179 kb on - strand at 1593.189 kb on - strand at 1593.189 kb on - strand at 1593.218 kb on + strand at 1593.459 kb on + strand, within Synpcc7942_1534 at 1593.514 kb on + strand, within Synpcc7942_1534 at 1593.576 kb on + strand, within Synpcc7942_1534 at 1593.628 kb on + strand, within Synpcc7942_1534 at 1593.655 kb on + strand, within Synpcc7942_1534 at 1593.742 kb on + strand, within Synpcc7942_1534 at 1593.742 kb on + strand, within Synpcc7942_1534 at 1593.750 kb on - strand, within Synpcc7942_1534 at 1593.809 kb on - strand, within Synpcc7942_1534 at 1593.824 kb on + strand, within Synpcc7942_1534 at 1593.861 kb on + strand, within Synpcc7942_1534 at 1594.088 kb on + strand, within Synpcc7942_1534 at 1594.088 kb on + strand, within Synpcc7942_1534
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 1,591,284 + modA Synpcc7942_1531 0.59 +1.0 1,591,373 - modA Synpcc7942_1531 0.70 -0.2 1,591,462 + modA Synpcc7942_1531 0.81 +1.4 1,591,559 - +1.0 1,591,628 - +0.8 1,591,628 - +2.0 1,592,051 + Synpcc7942_1532 0.66 -1.8 1,592,230 - -0.2 1,592,249 + +0.6 1,592,249 + +0.8 1,592,271 + +0.0 1,592,314 - -0.6 1,592,327 + +1.9 1,592,327 + +1.7 1,592,335 - -0.4 1,592,342 - sdhB Synpcc7942_1533 0.10 -1.6 1,592,390 + sdhB Synpcc7942_1533 0.16 +2.3 1,592,396 - sdhB Synpcc7942_1533 0.17 +2.1 1,592,398 - sdhB Synpcc7942_1533 0.17 +1.7 1,592,400 + sdhB Synpcc7942_1533 0.17 +2.0 1,592,400 + sdhB Synpcc7942_1533 0.17 +2.1 1,592,408 + sdhB Synpcc7942_1533 0.18 -0.1 1,592,408 - sdhB Synpcc7942_1533 0.18 +2.3 1,592,419 + sdhB Synpcc7942_1533 0.20 +2.9 1,592,445 + sdhB Synpcc7942_1533 0.23 +1.6 1,592,451 - sdhB Synpcc7942_1533 0.23 +2.0 1,592,460 - sdhB Synpcc7942_1533 0.25 +2.5 1,592,485 - sdhB Synpcc7942_1533 0.28 +2.2 1,592,489 + sdhB Synpcc7942_1533 0.28 +1.9 1,592,497 - sdhB Synpcc7942_1533 0.29 +2.2 1,592,551 - sdhB Synpcc7942_1533 0.35 +2.0 1,592,560 + sdhB Synpcc7942_1533 0.36 +1.1 1,592,596 + sdhB Synpcc7942_1533 0.41 +1.3 1,592,602 + sdhB Synpcc7942_1533 0.41 +2.4 1,592,647 - sdhB Synpcc7942_1533 0.47 +2.1 1,592,664 - sdhB Synpcc7942_1533 0.49 +3.0 1,592,664 - sdhB Synpcc7942_1533 0.49 +1.5 1,592,674 + sdhB Synpcc7942_1533 0.50 +3.0 1,592,674 + +2.1 1,592,681 - sdhB Synpcc7942_1533 0.51 +1.6 1,592,682 - sdhB Synpcc7942_1533 0.51 +2.7 1,592,721 + sdhB Synpcc7942_1533 0.56 +2.3 1,592,728 + sdhB Synpcc7942_1533 0.56 +2.2 1,592,733 - sdhB Synpcc7942_1533 0.57 +3.5 1,592,733 - sdhB Synpcc7942_1533 0.57 +2.4 1,592,795 - sdhB Synpcc7942_1533 0.64 +2.8 1,592,848 - sdhB Synpcc7942_1533 0.71 +2.2 1,592,950 - sdhB Synpcc7942_1533 0.83 +2.6 1,592,961 + sdhB Synpcc7942_1533 0.84 +2.7 1,592,982 - sdhB Synpcc7942_1533 0.87 +2.6 1,592,982 - sdhB Synpcc7942_1533 0.87 +2.0 1,593,037 + -2.0 1,593,042 + +2.6 1,593,055 - +0.7 1,593,096 - -0.5 1,593,123 + -1.0 1,593,123 + +0.1 1,593,140 + -1.5 1,593,171 + -0.4 1,593,171 + +0.5 1,593,179 - +1.0 1,593,179 - +0.4 1,593,179 - -2.5 1,593,179 - -1.7 1,593,179 - -0.2 1,593,179 - +0.0 1,593,189 - -0.2 1,593,189 - -2.3 1,593,218 + +0.0 1,593,459 + Synpcc7942_1534 0.11 +0.6 1,593,514 + Synpcc7942_1534 0.14 +1.6 1,593,576 + Synpcc7942_1534 0.16 +0.4 1,593,628 + Synpcc7942_1534 0.19 -1.2 1,593,655 + Synpcc7942_1534 0.20 -0.9 1,593,742 + Synpcc7942_1534 0.24 -1.4 1,593,742 + Synpcc7942_1534 0.24 +0.3 1,593,750 - Synpcc7942_1534 0.24 +0.4 1,593,809 - Synpcc7942_1534 0.27 -0.1 1,593,824 + Synpcc7942_1534 0.28 +0.5 1,593,861 + Synpcc7942_1534 0.29 +1.2 1,594,088 + Synpcc7942_1534 0.40 +0.2 1,594,088 + Synpcc7942_1534 0.40 -0.2
Or see this region's nucleotide sequence