Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mazG and Synpcc7942_1494 are separated by 28 nucleotides Synpcc7942_1494 and Synpcc7942_1495 are separated by 27 nucleotides Synpcc7942_1495 and argB overlap by 4 nucleotides argB and Synpcc7942_1497 overlap by 8 nucleotides
Synpcc7942_1493: mazG - nucleoside triphosphate pyrophosphohydrolase, at 1,544,514 to 1,545,353
mazG
Synpcc7942_1494: Synpcc7942_1494 - hypothetical protein, at 1,545,382 to 1,546,095
_1494
Synpcc7942_1495: Synpcc7942_1495 - hypothetical protein, at 1,546,123 to 1,546,650
_1495
Synpcc7942_1496: argB - acetylglutamate kinase, at 1,546,647 to 1,547,552
argB
Synpcc7942_1497: Synpcc7942_1497 - hypothetical protein, at 1,547,545 to 1,548,105
_1497
Position (kb)
1546
1547 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1545.131 kb on + strand, within mazG at 1545.139 kb on - strand, within mazG at 1545.139 kb on - strand, within mazG at 1545.143 kb on + strand, within mazG at 1545.144 kb on - strand, within mazG at 1545.167 kb on + strand, within mazG at 1545.175 kb on - strand, within mazG at 1545.203 kb on - strand, within mazG at 1545.207 kb on + strand, within mazG at 1545.207 kb on + strand, within mazG at 1545.209 kb on - strand, within mazG at 1545.210 kb on + strand, within mazG at 1545.215 kb on - strand, within mazG at 1545.223 kb on - strand, within mazG at 1545.270 kb on - strand at 1545.272 kb on + strand at 1545.282 kb on + strand at 1545.290 kb on - strand at 1545.331 kb on + strand at 1545.336 kb on + strand at 1545.348 kb on + strand at 1545.348 kb on + strand at 1545.348 kb on + strand at 1545.367 kb on - strand at 1545.380 kb on + strand at 1545.380 kb on + strand at 1545.395 kb on - strand at 1545.405 kb on - strand at 1545.565 kb on - strand, within Synpcc7942_1494 at 1546.101 kb on + strand at 1546.109 kb on - strand at 1546.109 kb on - strand at 1546.111 kb on - strand at 1546.111 kb on - strand at 1546.114 kb on + strand at 1546.119 kb on + strand at 1546.120 kb on + strand at 1546.127 kb on - strand at 1546.160 kb on + strand at 1546.168 kb on - strand at 1546.173 kb on + strand at 1546.173 kb on + strand at 1546.210 kb on - strand, within Synpcc7942_1495 at 1546.237 kb on - strand, within Synpcc7942_1495 at 1546.251 kb on - strand, within Synpcc7942_1495 at 1546.260 kb on - strand, within Synpcc7942_1495 at 1546.312 kb on - strand, within Synpcc7942_1495 at 1546.323 kb on + strand, within Synpcc7942_1495 at 1546.326 kb on - strand, within Synpcc7942_1495 at 1546.331 kb on - strand, within Synpcc7942_1495 at 1546.338 kb on - strand, within Synpcc7942_1495 at 1546.378 kb on + strand, within Synpcc7942_1495 at 1546.379 kb on + strand, within Synpcc7942_1495 at 1546.379 kb on + strand, within Synpcc7942_1495 at 1546.379 kb on + strand, within Synpcc7942_1495 at 1546.382 kb on - strand, within Synpcc7942_1495 at 1546.387 kb on - strand, within Synpcc7942_1495 at 1546.387 kb on - strand, within Synpcc7942_1495 at 1546.400 kb on - strand, within Synpcc7942_1495 at 1546.404 kb on + strand, within Synpcc7942_1495 at 1546.427 kb on - strand, within Synpcc7942_1495 at 1546.434 kb on + strand, within Synpcc7942_1495 at 1546.448 kb on + strand, within Synpcc7942_1495 at 1546.457 kb on + strand, within Synpcc7942_1495 at 1546.457 kb on + strand, within Synpcc7942_1495 at 1546.458 kb on + strand, within Synpcc7942_1495 at 1546.465 kb on - strand, within Synpcc7942_1495 at 1546.506 kb on + strand, within Synpcc7942_1495 at 1546.514 kb on - strand, within Synpcc7942_1495 at 1546.514 kb on - strand, within Synpcc7942_1495 at 1546.528 kb on - strand, within Synpcc7942_1495 at 1546.545 kb on - strand, within Synpcc7942_1495 at 1546.566 kb on - strand, within Synpcc7942_1495 at 1546.569 kb on + strand, within Synpcc7942_1495 at 1546.623 kb on - strand at 1546.623 kb on - strand at 1546.623 kb on - strand at 1546.623 kb on - strand at 1546.654 kb on - strand at 1547.600 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 1,545,131 + mazG Synpcc7942_1493 0.73 -0.5 1,545,139 - mazG Synpcc7942_1493 0.74 -0.5 1,545,139 - mazG Synpcc7942_1493 0.74 -0.2 1,545,143 + mazG Synpcc7942_1493 0.75 -0.4 1,545,144 - mazG Synpcc7942_1493 0.75 +1.4 1,545,167 + mazG Synpcc7942_1493 0.78 +1.1 1,545,175 - mazG Synpcc7942_1493 0.79 +0.1 1,545,203 - mazG Synpcc7942_1493 0.82 +0.2 1,545,207 + mazG Synpcc7942_1493 0.82 +0.5 1,545,207 + mazG Synpcc7942_1493 0.82 -1.3 1,545,209 - mazG Synpcc7942_1493 0.83 +0.4 1,545,210 + mazG Synpcc7942_1493 0.83 +0.9 1,545,215 - mazG Synpcc7942_1493 0.83 -3.3 1,545,223 - mazG Synpcc7942_1493 0.84 -0.3 1,545,270 - +0.3 1,545,272 + +0.6 1,545,282 + +0.6 1,545,290 - +0.6 1,545,331 + +1.8 1,545,336 + +0.4 1,545,348 + +0.4 1,545,348 + +1.1 1,545,348 + -2.5 1,545,367 - +0.2 1,545,380 + +0.3 1,545,380 + -1.4 1,545,395 - -0.0 1,545,405 - -1.0 1,545,565 - Synpcc7942_1494 0.26 -1.5 1,546,101 + +0.9 1,546,109 - +0.3 1,546,109 - +2.6 1,546,111 - -0.3 1,546,111 - -0.8 1,546,114 + -0.9 1,546,119 + -0.2 1,546,120 + -1.8 1,546,127 - -0.0 1,546,160 + -0.7 1,546,168 - -0.0 1,546,173 + -0.2 1,546,173 + -0.2 1,546,210 - Synpcc7942_1495 0.16 -2.9 1,546,237 - Synpcc7942_1495 0.22 -2.2 1,546,251 - Synpcc7942_1495 0.24 -2.3 1,546,260 - Synpcc7942_1495 0.26 -1.1 1,546,312 - Synpcc7942_1495 0.36 -0.2 1,546,323 + Synpcc7942_1495 0.38 -1.5 1,546,326 - Synpcc7942_1495 0.38 -0.6 1,546,331 - Synpcc7942_1495 0.39 -0.3 1,546,338 - Synpcc7942_1495 0.41 -0.1 1,546,378 + Synpcc7942_1495 0.48 -0.5 1,546,379 + Synpcc7942_1495 0.48 -1.0 1,546,379 + Synpcc7942_1495 0.48 -1.9 1,546,379 + Synpcc7942_1495 0.48 +0.6 1,546,382 - Synpcc7942_1495 0.49 +0.1 1,546,387 - Synpcc7942_1495 0.50 -0.1 1,546,387 - Synpcc7942_1495 0.50 -0.0 1,546,400 - Synpcc7942_1495 0.52 -0.3 1,546,404 + Synpcc7942_1495 0.53 -0.3 1,546,427 - Synpcc7942_1495 0.58 -0.1 1,546,434 + Synpcc7942_1495 0.59 -2.0 1,546,448 + Synpcc7942_1495 0.62 -0.5 1,546,457 + Synpcc7942_1495 0.63 -0.3 1,546,457 + Synpcc7942_1495 0.63 -1.7 1,546,458 + Synpcc7942_1495 0.63 -0.9 1,546,465 - Synpcc7942_1495 0.65 +0.4 1,546,506 + Synpcc7942_1495 0.73 +0.1 1,546,514 - Synpcc7942_1495 0.74 -3.9 1,546,514 - Synpcc7942_1495 0.74 +0.0 1,546,528 - Synpcc7942_1495 0.77 -1.2 1,546,545 - Synpcc7942_1495 0.80 -0.3 1,546,566 - Synpcc7942_1495 0.84 +0.7 1,546,569 + Synpcc7942_1495 0.84 -1.8 1,546,623 - -1.2 1,546,623 - +0.4 1,546,623 - +0.1 1,546,623 - +0.1 1,546,654 - -0.7 1,547,600 + +1.6
Or see this region's nucleotide sequence