Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt murE and Synpcc7942_1485 are separated by 39 nucleotides Synpcc7942_1485 and Synpcc7942_1486 overlap by 10 nucleotides Synpcc7942_1486 and rpsD are separated by 73 nucleotides
Synpcc7942_1484: murE - UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase, at 1,534,874 to 1,536,367
murE
Synpcc7942_1485: Synpcc7942_1485 - hypothetical protein, at 1,536,407 to 1,536,673
_1485
Synpcc7942_1486: Synpcc7942_1486 - Protein of unknown function DUF37, at 1,536,664 to 1,536,912
_1486
Synpcc7942_1487: rpsD - 30S ribosomal protein S4, at 1,536,986 to 1,537,594
rpsD
Position (kb)
1536
1537 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1536.382 kb on - strand at 1536.387 kb on - strand at 1536.405 kb on + strand at 1536.405 kb on + strand at 1536.413 kb on - strand at 1536.448 kb on + strand, within Synpcc7942_1485 at 1536.474 kb on - strand, within Synpcc7942_1485 at 1536.556 kb on - strand, within Synpcc7942_1485 at 1536.561 kb on - strand, within Synpcc7942_1485 at 1536.623 kb on - strand, within Synpcc7942_1485 at 1536.625 kb on + strand, within Synpcc7942_1485 at 1536.638 kb on - strand, within Synpcc7942_1485 at 1536.656 kb on + strand at 1536.659 kb on - strand at 1536.664 kb on - strand at 1536.670 kb on - strand at 1536.689 kb on + strand, within Synpcc7942_1486 at 1536.714 kb on + strand, within Synpcc7942_1486 at 1536.737 kb on + strand, within Synpcc7942_1486 at 1536.753 kb on - strand at 1536.770 kb on - strand, within Synpcc7942_1486 at 1536.783 kb on - strand, within Synpcc7942_1486 at 1536.801 kb on - strand, within Synpcc7942_1486 at 1536.804 kb on + strand, within Synpcc7942_1486 at 1536.804 kb on + strand, within Synpcc7942_1486 at 1536.804 kb on + strand, within Synpcc7942_1486 at 1536.804 kb on + strand, within Synpcc7942_1486 at 1536.804 kb on + strand, within Synpcc7942_1486 at 1536.804 kb on + strand, within Synpcc7942_1486 at 1536.804 kb on + strand, within Synpcc7942_1486 at 1536.804 kb on + strand, within Synpcc7942_1486 at 1536.812 kb on - strand, within Synpcc7942_1486 at 1536.812 kb on - strand, within Synpcc7942_1486 at 1536.812 kb on - strand, within Synpcc7942_1486 at 1536.812 kb on - strand, within Synpcc7942_1486 at 1536.816 kb on + strand, within Synpcc7942_1486 at 1536.826 kb on + strand, within Synpcc7942_1486 at 1536.826 kb on + strand, within Synpcc7942_1486 at 1536.838 kb on - strand, within Synpcc7942_1486 at 1536.891 kb on + strand at 1537.615 kb on - strand at 1537.615 kb on - strand at 1537.637 kb on + strand at 1537.646 kb on - strand at 1537.648 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 1,536,382 - +0.1 1,536,387 - +0.4 1,536,405 + +1.0 1,536,405 + +0.6 1,536,413 - +0.2 1,536,448 + Synpcc7942_1485 0.15 -1.0 1,536,474 - Synpcc7942_1485 0.25 -0.9 1,536,556 - Synpcc7942_1485 0.56 -1.0 1,536,561 - Synpcc7942_1485 0.58 -1.3 1,536,623 - Synpcc7942_1485 0.81 -0.1 1,536,625 + Synpcc7942_1485 0.82 -0.3 1,536,638 - Synpcc7942_1485 0.87 -0.8 1,536,656 + -0.3 1,536,659 - -0.6 1,536,664 - -0.8 1,536,670 - -0.6 1,536,689 + Synpcc7942_1486 0.10 -0.3 1,536,714 + Synpcc7942_1486 0.20 +0.6 1,536,737 + Synpcc7942_1486 0.29 -0.1 1,536,753 - -2.2 1,536,770 - Synpcc7942_1486 0.43 -1.0 1,536,783 - Synpcc7942_1486 0.48 -0.4 1,536,801 - Synpcc7942_1486 0.55 -1.1 1,536,804 + Synpcc7942_1486 0.56 +0.2 1,536,804 + Synpcc7942_1486 0.56 +0.4 1,536,804 + Synpcc7942_1486 0.56 +0.2 1,536,804 + Synpcc7942_1486 0.56 -0.0 1,536,804 + Synpcc7942_1486 0.56 -0.5 1,536,804 + Synpcc7942_1486 0.56 -0.0 1,536,804 + Synpcc7942_1486 0.56 -1.6 1,536,804 + Synpcc7942_1486 0.56 -0.4 1,536,812 - Synpcc7942_1486 0.59 +0.1 1,536,812 - Synpcc7942_1486 0.59 +0.3 1,536,812 - Synpcc7942_1486 0.59 -1.8 1,536,812 - Synpcc7942_1486 0.59 -2.9 1,536,816 + Synpcc7942_1486 0.61 +0.6 1,536,826 + Synpcc7942_1486 0.65 -0.7 1,536,826 + Synpcc7942_1486 0.65 -1.7 1,536,838 - Synpcc7942_1486 0.70 -2.0 1,536,891 + +0.3 1,537,615 - -0.3 1,537,615 - -1.7 1,537,637 + +0.0 1,537,646 - +1.3 1,537,648 + -0.5
Or see this region's nucleotide sequence