Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sbtA and Synpcc7942_1476 are separated by 762 nucleotides Synpcc7942_1476 and Synpcc7942_1477 are separated by 110 nucleotides
Synpcc7942_1475: sbtA - sodium-dependent bicarbonate transporter, at 1,525,833 to 1,526,954
sbtA
Synpcc7942_1476: Synpcc7942_1476 - hypothetical protein, at 1,527,717 to 1,528,031
_1476
Synpcc7942_1477: Synpcc7942_1477 - hypothetical protein, at 1,528,142 to 1,530,337
_1477
Position (kb)
1527
1528
1529 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1526.737 kb on + strand, within sbtA at 1527.134 kb on + strand at 1527.383 kb on - strand at 1527.383 kb on - strand at 1527.395 kb on + strand at 1527.428 kb on - strand at 1527.438 kb on - strand at 1527.438 kb on - strand at 1527.438 kb on - strand at 1527.438 kb on - strand at 1527.463 kb on + strand at 1527.528 kb on - strand at 1527.534 kb on + strand at 1527.592 kb on + strand at 1527.610 kb on + strand at 1527.626 kb on + strand at 1527.746 kb on - strand at 1527.806 kb on + strand, within Synpcc7942_1476 at 1527.807 kb on + strand, within Synpcc7942_1476 at 1527.814 kb on - strand, within Synpcc7942_1476 at 1527.814 kb on - strand, within Synpcc7942_1476 at 1527.826 kb on - strand, within Synpcc7942_1476 at 1527.849 kb on - strand, within Synpcc7942_1476 at 1527.856 kb on + strand, within Synpcc7942_1476 at 1527.856 kb on + strand, within Synpcc7942_1476 at 1527.881 kb on + strand, within Synpcc7942_1476 at 1527.992 kb on - strand, within Synpcc7942_1476 at 1527.999 kb on + strand, within Synpcc7942_1476 at 1528.016 kb on - strand at 1528.046 kb on + strand at 1528.054 kb on - strand at 1528.054 kb on - strand at 1528.061 kb on - strand at 1528.083 kb on - strand at 1528.112 kb on - strand at 1528.114 kb on + strand at 1528.117 kb on - strand at 1528.122 kb on - strand at 1528.141 kb on - strand at 1528.146 kb on - strand at 1528.193 kb on + strand at 1528.201 kb on - strand at 1528.201 kb on - strand at 1528.253 kb on + strand at 1528.261 kb on - strand at 1528.312 kb on + strand at 1528.325 kb on - strand at 1528.331 kb on + strand at 1528.399 kb on + strand, within Synpcc7942_1477 at 1528.400 kb on + strand, within Synpcc7942_1477 at 1528.407 kb on - strand, within Synpcc7942_1477 at 1528.425 kb on + strand, within Synpcc7942_1477 at 1528.433 kb on - strand, within Synpcc7942_1477 at 1528.497 kb on - strand, within Synpcc7942_1477 at 1528.502 kb on - strand, within Synpcc7942_1477 at 1528.532 kb on - strand, within Synpcc7942_1477 at 1528.581 kb on + strand, within Synpcc7942_1477 at 1528.599 kb on - strand, within Synpcc7942_1477 at 1528.621 kb on + strand, within Synpcc7942_1477 at 1528.627 kb on - strand, within Synpcc7942_1477 at 1528.629 kb on - strand, within Synpcc7942_1477 at 1528.629 kb on - strand, within Synpcc7942_1477 at 1528.629 kb on - strand, within Synpcc7942_1477 at 1528.631 kb on + strand, within Synpcc7942_1477 at 1528.639 kb on - strand, within Synpcc7942_1477 at 1528.641 kb on - strand, within Synpcc7942_1477 at 1528.659 kb on - strand, within Synpcc7942_1477 at 1528.740 kb on + strand, within Synpcc7942_1477 at 1528.742 kb on + strand, within Synpcc7942_1477 at 1528.748 kb on - strand, within Synpcc7942_1477 at 1528.748 kb on - strand, within Synpcc7942_1477 at 1528.751 kb on + strand, within Synpcc7942_1477 at 1528.759 kb on - strand, within Synpcc7942_1477 at 1528.759 kb on - strand, within Synpcc7942_1477 at 1528.761 kb on - strand, within Synpcc7942_1477 at 1528.771 kb on - strand, within Synpcc7942_1477 at 1528.791 kb on - strand, within Synpcc7942_1477 at 1528.810 kb on + strand, within Synpcc7942_1477 at 1528.826 kb on + strand, within Synpcc7942_1477 at 1528.859 kb on - strand, within Synpcc7942_1477 at 1528.909 kb on + strand, within Synpcc7942_1477 at 1528.943 kb on - strand, within Synpcc7942_1477 at 1528.944 kb on - strand, within Synpcc7942_1477 at 1528.944 kb on - strand, within Synpcc7942_1477 at 1528.947 kb on + strand, within Synpcc7942_1477 at 1528.954 kb on + strand, within Synpcc7942_1477 at 1528.955 kb on - strand, within Synpcc7942_1477 at 1528.955 kb on - strand, within Synpcc7942_1477 at 1528.959 kb on + strand, within Synpcc7942_1477 at 1528.975 kb on + strand, within Synpcc7942_1477 at 1528.979 kb on + strand, within Synpcc7942_1477 at 1529.004 kb on - strand, within Synpcc7942_1477 at 1529.018 kb on + strand, within Synpcc7942_1477
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 1,526,737 + sbtA Synpcc7942_1475 0.81 +2.9 1,527,134 + +0.3 1,527,383 - +0.8 1,527,383 - -1.7 1,527,395 + -0.5 1,527,428 - -0.7 1,527,438 - -2.2 1,527,438 - -1.6 1,527,438 - -0.8 1,527,438 - -2.1 1,527,463 + -0.8 1,527,528 - -0.7 1,527,534 + +0.8 1,527,592 + -1.9 1,527,610 + +0.2 1,527,626 + +2.7 1,527,746 - -3.7 1,527,806 + Synpcc7942_1476 0.28 -3.9 1,527,807 + Synpcc7942_1476 0.29 -3.2 1,527,814 - Synpcc7942_1476 0.31 -1.9 1,527,814 - Synpcc7942_1476 0.31 -3.8 1,527,826 - Synpcc7942_1476 0.35 -2.3 1,527,849 - Synpcc7942_1476 0.42 -2.7 1,527,856 + Synpcc7942_1476 0.44 -0.9 1,527,856 + Synpcc7942_1476 0.44 -3.3 1,527,881 + Synpcc7942_1476 0.52 -1.0 1,527,992 - Synpcc7942_1476 0.87 -0.0 1,527,999 + Synpcc7942_1476 0.90 -0.9 1,528,016 - -3.2 1,528,046 + -2.6 1,528,054 - -1.8 1,528,054 - -0.5 1,528,061 - -2.7 1,528,083 - -1.6 1,528,112 - -1.6 1,528,114 + -1.0 1,528,117 - -2.0 1,528,122 - -1.6 1,528,141 - -0.6 1,528,146 - -2.3 1,528,193 + -0.6 1,528,201 - -1.6 1,528,201 - -0.2 1,528,253 + +0.4 1,528,261 - -1.0 1,528,312 + +0.5 1,528,325 - -0.6 1,528,331 + -0.1 1,528,399 + Synpcc7942_1477 0.12 -0.2 1,528,400 + Synpcc7942_1477 0.12 -1.3 1,528,407 - Synpcc7942_1477 0.12 -0.2 1,528,425 + Synpcc7942_1477 0.13 -1.7 1,528,433 - Synpcc7942_1477 0.13 -1.8 1,528,497 - Synpcc7942_1477 0.16 -0.7 1,528,502 - Synpcc7942_1477 0.16 +0.8 1,528,532 - Synpcc7942_1477 0.18 -1.7 1,528,581 + Synpcc7942_1477 0.20 +0.2 1,528,599 - Synpcc7942_1477 0.21 +0.5 1,528,621 + Synpcc7942_1477 0.22 +0.3 1,528,627 - Synpcc7942_1477 0.22 +0.0 1,528,629 - Synpcc7942_1477 0.22 -0.5 1,528,629 - Synpcc7942_1477 0.22 -1.5 1,528,629 - Synpcc7942_1477 0.22 -1.5 1,528,631 + Synpcc7942_1477 0.22 +0.2 1,528,639 - Synpcc7942_1477 0.23 +0.4 1,528,641 - Synpcc7942_1477 0.23 +1.0 1,528,659 - Synpcc7942_1477 0.24 -0.0 1,528,740 + Synpcc7942_1477 0.27 +0.0 1,528,742 + Synpcc7942_1477 0.27 +0.1 1,528,748 - Synpcc7942_1477 0.28 +0.6 1,528,748 - Synpcc7942_1477 0.28 -1.6 1,528,751 + Synpcc7942_1477 0.28 -0.5 1,528,759 - Synpcc7942_1477 0.28 +0.7 1,528,759 - Synpcc7942_1477 0.28 -1.0 1,528,761 - Synpcc7942_1477 0.28 -0.3 1,528,771 - Synpcc7942_1477 0.29 +0.2 1,528,791 - Synpcc7942_1477 0.30 +0.5 1,528,810 + Synpcc7942_1477 0.30 -2.2 1,528,826 + Synpcc7942_1477 0.31 -1.8 1,528,859 - Synpcc7942_1477 0.33 -0.3 1,528,909 + Synpcc7942_1477 0.35 -0.3 1,528,943 - Synpcc7942_1477 0.36 -0.0 1,528,944 - Synpcc7942_1477 0.37 +0.3 1,528,944 - Synpcc7942_1477 0.37 +0.3 1,528,947 + Synpcc7942_1477 0.37 +0.3 1,528,954 + Synpcc7942_1477 0.37 +0.2 1,528,955 - Synpcc7942_1477 0.37 +0.2 1,528,955 - Synpcc7942_1477 0.37 -0.2 1,528,959 + Synpcc7942_1477 0.37 -0.6 1,528,975 + Synpcc7942_1477 0.38 -1.0 1,528,979 + Synpcc7942_1477 0.38 +0.7 1,529,004 - Synpcc7942_1477 0.39 +0.2 1,529,018 + Synpcc7942_1477 0.40 +1.6
Or see this region's nucleotide sequence