Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cobD and Synpcc7942_1442 are separated by 247 nucleotides Synpcc7942_1442 and tRNA-Lys are separated by 70 nucleotides tRNA-Lys and fba are separated by 241 nucleotides
Synpcc7942_1441: cobD - cobalamin biosynthesis protein, at 1,495,174 to 1,496,151
cobD
Synpcc7942_1442: Synpcc7942_1442 - hypothetical protein, at 1,496,399 to 1,496,833
_1442
Synpcc7942_R0049: tRNA-Lys - tRNA-Lys, at 1,496,904 to 1,496,975
tRNA-Lys
Synpcc7942_1443: fba - fructose-1,6-bisphosphate aldolase, at 1,497,217 to 1,498,290
fba
Position (kb)
1496
1497 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1496.146 kb on + strand at 1496.146 kb on + strand at 1496.154 kb on - strand at 1496.154 kb on - strand at 1496.157 kb on + strand at 1496.165 kb on - strand at 1496.165 kb on - strand at 1496.198 kb on + strand at 1496.198 kb on + strand at 1496.226 kb on + strand at 1496.229 kb on - strand at 1496.234 kb on - strand at 1496.239 kb on - strand at 1496.299 kb on + strand at 1496.299 kb on + strand at 1496.347 kb on + strand at 1496.354 kb on - strand at 1496.364 kb on - strand at 1496.379 kb on + strand at 1496.404 kb on - strand at 1496.404 kb on - strand at 1496.456 kb on - strand, within Synpcc7942_1442 at 1496.470 kb on + strand, within Synpcc7942_1442 at 1496.519 kb on + strand, within Synpcc7942_1442 at 1496.520 kb on + strand, within Synpcc7942_1442 at 1496.520 kb on + strand, within Synpcc7942_1442 at 1496.527 kb on - strand, within Synpcc7942_1442 at 1496.532 kb on + strand, within Synpcc7942_1442 at 1496.557 kb on + strand, within Synpcc7942_1442 at 1496.559 kb on - strand, within Synpcc7942_1442 at 1496.590 kb on + strand, within Synpcc7942_1442 at 1496.593 kb on + strand, within Synpcc7942_1442 at 1496.598 kb on - strand, within Synpcc7942_1442 at 1496.608 kb on - strand, within Synpcc7942_1442 at 1496.637 kb on + strand, within Synpcc7942_1442 at 1496.645 kb on - strand, within Synpcc7942_1442 at 1496.692 kb on - strand, within Synpcc7942_1442 at 1496.718 kb on + strand, within Synpcc7942_1442 at 1496.726 kb on - strand, within Synpcc7942_1442 at 1496.794 kb on - strand at 1496.997 kb on + strand at 1497.005 kb on - strand at 1497.023 kb on + strand at 1497.031 kb on - strand at 1497.114 kb on + strand at 1497.138 kb on - strand at 1497.138 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 1,496,146 + +0.6 1,496,146 + -1.6 1,496,154 - -0.6 1,496,154 - +0.1 1,496,157 + -1.5 1,496,165 - -0.3 1,496,165 - -1.2 1,496,198 + +1.2 1,496,198 + -0.9 1,496,226 + -1.0 1,496,229 - -1.3 1,496,234 - -1.3 1,496,239 - +0.1 1,496,299 + -0.2 1,496,299 + +0.1 1,496,347 + -0.6 1,496,354 - -1.3 1,496,364 - -0.9 1,496,379 + -0.6 1,496,404 - +0.6 1,496,404 - +0.7 1,496,456 - Synpcc7942_1442 0.13 -2.5 1,496,470 + Synpcc7942_1442 0.16 -1.4 1,496,519 + Synpcc7942_1442 0.28 -2.3 1,496,520 + Synpcc7942_1442 0.28 -0.9 1,496,520 + Synpcc7942_1442 0.28 -1.3 1,496,527 - Synpcc7942_1442 0.29 +1.0 1,496,532 + Synpcc7942_1442 0.31 -3.5 1,496,557 + Synpcc7942_1442 0.36 -0.3 1,496,559 - Synpcc7942_1442 0.37 -1.2 1,496,590 + Synpcc7942_1442 0.44 -1.4 1,496,593 + Synpcc7942_1442 0.45 -3.0 1,496,598 - Synpcc7942_1442 0.46 -2.3 1,496,608 - Synpcc7942_1442 0.48 -1.4 1,496,637 + Synpcc7942_1442 0.55 -0.9 1,496,645 - Synpcc7942_1442 0.57 -1.7 1,496,692 - Synpcc7942_1442 0.67 -0.8 1,496,718 + Synpcc7942_1442 0.73 -0.6 1,496,726 - Synpcc7942_1442 0.75 -1.5 1,496,794 - -0.2 1,496,997 + -0.8 1,497,005 - -0.4 1,497,023 + -3.7 1,497,031 - -1.2 1,497,114 + -0.9 1,497,138 - -0.0 1,497,138 - -0.1
Or see this region's nucleotide sequence