Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt topA and Synpcc7942_1417 are separated by 4 nucleotides Synpcc7942_1417 and ccmJ overlap by 4 nucleotides ccmJ and chlL are separated by 398 nucleotides
Synpcc7942_1416: topA - DNA topoisomerase I, at 1,468,344 to 1,470,995
topA
Synpcc7942_1417: Synpcc7942_1417 - hypothetical protein, at 1,471,000 to 1,471,983
_1417
Synpcc7942_1418: ccmJ - hypothetical protein, at 1,471,980 to 1,472,186
ccmJ
Synpcc7942_1419: chlL - protochlorophyllide reductase iron-sulfur ATP-binding protein, at 1,472,585 to 1,473,445
chlL
Position (kb)
1470
1471
1472 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1470.715 kb on + strand, within topA at 1470.743 kb on - strand at 1470.794 kb on + strand at 1470.881 kb on + strand at 1470.881 kb on + strand at 1470.887 kb on + strand at 1470.887 kb on + strand at 1470.887 kb on + strand at 1470.895 kb on - strand at 1470.895 kb on - strand at 1470.895 kb on - strand at 1470.895 kb on - strand at 1470.895 kb on - strand at 1470.895 kb on - strand at 1470.895 kb on - strand at 1470.916 kb on + strand at 1470.990 kb on + strand at 1470.990 kb on + strand at 1470.990 kb on + strand at 1471.011 kb on + strand at 1471.018 kb on - strand at 1471.104 kb on + strand, within Synpcc7942_1417 at 1471.104 kb on - strand, within Synpcc7942_1417 at 1471.165 kb on + strand, within Synpcc7942_1417 at 1471.173 kb on - strand, within Synpcc7942_1417 at 1471.178 kb on - strand, within Synpcc7942_1417 at 1471.183 kb on - strand, within Synpcc7942_1417 at 1471.185 kb on + strand, within Synpcc7942_1417 at 1471.193 kb on - strand, within Synpcc7942_1417 at 1471.195 kb on + strand, within Synpcc7942_1417 at 1471.203 kb on - strand, within Synpcc7942_1417 at 1471.204 kb on - strand, within Synpcc7942_1417 at 1471.264 kb on + strand, within Synpcc7942_1417 at 1471.267 kb on - strand, within Synpcc7942_1417 at 1471.280 kb on + strand, within Synpcc7942_1417 at 1471.280 kb on + strand, within Synpcc7942_1417 at 1471.288 kb on - strand, within Synpcc7942_1417 at 1471.288 kb on - strand, within Synpcc7942_1417 at 1471.469 kb on + strand, within Synpcc7942_1417 at 1471.513 kb on + strand, within Synpcc7942_1417 at 1471.521 kb on - strand, within Synpcc7942_1417 at 1471.548 kb on - strand, within Synpcc7942_1417 at 1471.565 kb on - strand, within Synpcc7942_1417 at 1471.567 kb on + strand, within Synpcc7942_1417 at 1471.567 kb on + strand, within Synpcc7942_1417 at 1471.567 kb on + strand, within Synpcc7942_1417 at 1471.567 kb on + strand, within Synpcc7942_1417 at 1471.575 kb on - strand, within Synpcc7942_1417 at 1471.575 kb on - strand, within Synpcc7942_1417 at 1471.575 kb on - strand, within Synpcc7942_1417 at 1471.575 kb on - strand, within Synpcc7942_1417 at 1471.580 kb on - strand, within Synpcc7942_1417 at 1471.582 kb on + strand, within Synpcc7942_1417 at 1471.590 kb on + strand, within Synpcc7942_1417 at 1471.590 kb on + strand, within Synpcc7942_1417 at 1471.590 kb on - strand, within Synpcc7942_1417 at 1471.590 kb on - strand, within Synpcc7942_1417 at 1471.591 kb on - strand, within Synpcc7942_1417 at 1471.593 kb on + strand, within Synpcc7942_1417 at 1471.598 kb on - strand, within Synpcc7942_1417 at 1471.598 kb on - strand, within Synpcc7942_1417 at 1471.616 kb on + strand, within Synpcc7942_1417 at 1471.622 kb on - strand, within Synpcc7942_1417 at 1471.624 kb on - strand, within Synpcc7942_1417 at 1471.634 kb on - strand, within Synpcc7942_1417 at 1471.690 kb on - strand, within Synpcc7942_1417 at 1471.808 kb on - strand, within Synpcc7942_1417 at 1471.808 kb on - strand, within Synpcc7942_1417 at 1471.845 kb on + strand, within Synpcc7942_1417 at 1471.866 kb on - strand, within Synpcc7942_1417 at 1471.871 kb on - strand, within Synpcc7942_1417 at 1471.891 kb on - strand at 1471.892 kb on - strand at 1471.907 kb on - strand at 1471.918 kb on - strand at 1471.958 kb on + strand at 1471.965 kb on + strand at 1471.972 kb on - strand at 1471.973 kb on - strand at 1471.977 kb on + strand at 1471.977 kb on + strand at 1471.977 kb on + strand at 1471.979 kb on - strand at 1471.985 kb on - strand at 1471.985 kb on - strand at 1471.985 kb on - strand at 1471.985 kb on - strand at 1471.993 kb on + strand at 1471.993 kb on + strand at 1471.993 kb on + strand at 1472.000 kb on + strand at 1472.001 kb on - strand, within ccmJ at 1472.001 kb on - strand, within ccmJ at 1472.001 kb on - strand, within ccmJ at 1472.012 kb on + strand, within ccmJ at 1472.063 kb on + strand, within ccmJ at 1472.085 kb on + strand, within ccmJ at 1472.095 kb on + strand, within ccmJ at 1472.143 kb on - strand, within ccmJ at 1472.182 kb on + strand at 1472.182 kb on + strand at 1472.182 kb on + strand at 1472.190 kb on - strand at 1472.190 kb on - strand at 1472.242 kb on - strand at 1472.275 kb on + strand at 1472.327 kb on - strand at 1472.490 kb on + strand at 1472.490 kb on + strand at 1472.498 kb on - strand at 1472.498 kb on - strand at 1472.574 kb on - strand at 1472.696 kb on - strand, within chlL at 1472.746 kb on + strand, within chlL at 1472.767 kb on + strand, within chlL at 1472.785 kb on + strand, within chlL at 1472.866 kb on - strand, within chlL
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 1,470,715 + topA Synpcc7942_1416 0.89 +1.0 1,470,743 - +2.6 1,470,794 + +1.3 1,470,881 + -1.0 1,470,881 + -0.1 1,470,887 + -0.8 1,470,887 + +0.0 1,470,887 + +0.1 1,470,895 - -1.2 1,470,895 - +1.8 1,470,895 - +1.2 1,470,895 - -0.0 1,470,895 - +2.0 1,470,895 - +2.2 1,470,895 - +2.0 1,470,916 + -3.8 1,470,990 + -0.7 1,470,990 + -0.8 1,470,990 + +0.0 1,471,011 + -1.6 1,471,018 - -0.5 1,471,104 + Synpcc7942_1417 0.11 -0.4 1,471,104 - Synpcc7942_1417 0.11 -1.4 1,471,165 + Synpcc7942_1417 0.17 -2.1 1,471,173 - Synpcc7942_1417 0.18 -0.6 1,471,178 - Synpcc7942_1417 0.18 -2.2 1,471,183 - Synpcc7942_1417 0.19 -1.5 1,471,185 + Synpcc7942_1417 0.19 +0.1 1,471,193 - Synpcc7942_1417 0.20 -0.7 1,471,195 + Synpcc7942_1417 0.20 -0.3 1,471,203 - Synpcc7942_1417 0.21 -1.4 1,471,204 - Synpcc7942_1417 0.21 -1.8 1,471,264 + Synpcc7942_1417 0.27 -1.2 1,471,267 - Synpcc7942_1417 0.27 -1.4 1,471,280 + Synpcc7942_1417 0.28 -1.0 1,471,280 + Synpcc7942_1417 0.28 +0.2 1,471,288 - Synpcc7942_1417 0.29 -1.3 1,471,288 - Synpcc7942_1417 0.29 -1.6 1,471,469 + Synpcc7942_1417 0.48 -1.3 1,471,513 + Synpcc7942_1417 0.52 +0.2 1,471,521 - Synpcc7942_1417 0.53 -0.8 1,471,548 - Synpcc7942_1417 0.56 -0.8 1,471,565 - Synpcc7942_1417 0.57 -0.5 1,471,567 + Synpcc7942_1417 0.58 -0.6 1,471,567 + Synpcc7942_1417 0.58 -1.0 1,471,567 + Synpcc7942_1417 0.58 -0.7 1,471,567 + Synpcc7942_1417 0.58 -1.8 1,471,575 - Synpcc7942_1417 0.58 -0.6 1,471,575 - Synpcc7942_1417 0.58 -1.8 1,471,575 - Synpcc7942_1417 0.58 -0.6 1,471,575 - Synpcc7942_1417 0.58 -0.4 1,471,580 - Synpcc7942_1417 0.59 -2.4 1,471,582 + Synpcc7942_1417 0.59 -1.8 1,471,590 + Synpcc7942_1417 0.60 -3.3 1,471,590 + Synpcc7942_1417 0.60 -0.8 1,471,590 - Synpcc7942_1417 0.60 -1.4 1,471,590 - Synpcc7942_1417 0.60 -1.4 1,471,591 - Synpcc7942_1417 0.60 -2.8 1,471,593 + Synpcc7942_1417 0.60 -1.0 1,471,598 - Synpcc7942_1417 0.61 -0.2 1,471,598 - Synpcc7942_1417 0.61 -0.8 1,471,616 + Synpcc7942_1417 0.63 -2.6 1,471,622 - Synpcc7942_1417 0.63 -0.8 1,471,624 - Synpcc7942_1417 0.63 -1.1 1,471,634 - Synpcc7942_1417 0.64 +0.3 1,471,690 - Synpcc7942_1417 0.70 -0.4 1,471,808 - Synpcc7942_1417 0.82 -0.0 1,471,808 - Synpcc7942_1417 0.82 -0.4 1,471,845 + Synpcc7942_1417 0.86 -2.8 1,471,866 - Synpcc7942_1417 0.88 -0.4 1,471,871 - Synpcc7942_1417 0.89 -1.0 1,471,891 - +0.5 1,471,892 - +0.2 1,471,907 - -0.6 1,471,918 - -2.3 1,471,958 + +0.2 1,471,965 + -0.6 1,471,972 - -0.4 1,471,973 - +0.4 1,471,977 + -0.8 1,471,977 + -0.1 1,471,977 + +0.1 1,471,979 - +0.4 1,471,985 - -0.2 1,471,985 - -0.7 1,471,985 - +1.9 1,471,985 - -0.4 1,471,993 + +1.0 1,471,993 + -0.0 1,471,993 + -0.3 1,472,000 + +1.0 1,472,001 - ccmJ Synpcc7942_1418 0.10 +0.4 1,472,001 - ccmJ Synpcc7942_1418 0.10 -0.8 1,472,001 - ccmJ Synpcc7942_1418 0.10 -0.6 1,472,012 + ccmJ Synpcc7942_1418 0.15 +1.8 1,472,063 + ccmJ Synpcc7942_1418 0.40 -0.2 1,472,085 + ccmJ Synpcc7942_1418 0.51 +0.4 1,472,095 + ccmJ Synpcc7942_1418 0.56 -0.5 1,472,143 - ccmJ Synpcc7942_1418 0.79 +0.1 1,472,182 + +0.6 1,472,182 + -1.5 1,472,182 + -0.1 1,472,190 - -0.0 1,472,190 - -0.3 1,472,242 - -1.5 1,472,275 + +1.2 1,472,327 - +1.8 1,472,490 + +0.3 1,472,490 + -0.1 1,472,498 - -0.6 1,472,498 - +0.1 1,472,574 - +0.4 1,472,696 - chlL Synpcc7942_1419 0.13 +0.2 1,472,746 + chlL Synpcc7942_1419 0.19 +0.6 1,472,767 + chlL Synpcc7942_1419 0.21 -0.0 1,472,785 + chlL Synpcc7942_1419 0.23 -0.2 1,472,866 - chlL Synpcc7942_1419 0.33 +0.7
Or see this region's nucleotide sequence