Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kaiC and kaiB are separated by 49 nucleotides kaiB and kaiA are separated by 89 nucleotides kaiA and rpl21 are separated by 237 nucleotides rpl21 and rpl27 are separated by 34 nucleotides
Synpcc7942_1216: kaiC - circadian clock protein KaiC, at 1,238,550 to 1,240,109
kaiC
Synpcc7942_1217: kaiB - circadian clock protein KaiB, at 1,240,159 to 1,240,467
kaiB
Synpcc7942_1218: kaiA - circadian clock protein KaiA, at 1,240,557 to 1,241,411
kaiA
Synpcc7942_1219: rpl21 - 50S ribosomal protein L21, at 1,241,649 to 1,242,032
rpl21
Synpcc7942_1220: rpl27 - 50S ribosomal protein L27, at 1,242,067 to 1,242,333
rpl27
Position (kb) 1240
1241
1242 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 1239.567 kb on + strand, within kaiC at 1239.567 kb on + strand, within kaiC at 1239.659 kb on + strand, within kaiC at 1239.755 kb on + strand, within kaiC at 1239.761 kb on + strand, within kaiC at 1239.770 kb on - strand, within kaiC at 1239.914 kb on + strand, within kaiC at 1240.261 kb on + strand, within kaiB at 1240.443 kb on - strand at 1240.463 kb on + strand at 1240.463 kb on + strand at 1240.543 kb on - strand at 1240.772 kb on + strand, within kaiA at 1240.827 kb on + strand, within kaiA at 1240.834 kb on + strand, within kaiA at 1240.907 kb on + strand, within kaiA at 1240.925 kb on - strand, within kaiA at 1240.934 kb on - strand, within kaiA at 1240.934 kb on - strand, within kaiA at 1240.936 kb on - strand, within kaiA at 1240.966 kb on - strand, within kaiA at 1240.972 kb on + strand, within kaiA at 1240.989 kb on + strand, within kaiA at 1241.021 kb on - strand, within kaiA at 1241.021 kb on - strand, within kaiA at 1241.048 kb on + strand, within kaiA at 1241.053 kb on + strand, within kaiA at 1241.056 kb on - strand, within kaiA at 1241.065 kb on - strand, within kaiA at 1241.065 kb on - strand, within kaiA at 1241.092 kb on - strand, within kaiA at 1241.097 kb on + strand, within kaiA at 1241.097 kb on + strand, within kaiA at 1241.100 kb on - strand, within kaiA at 1241.102 kb on + strand, within kaiA at 1241.102 kb on + strand, within kaiA at 1241.121 kb on - strand, within kaiA at 1241.155 kb on - strand, within kaiA at 1241.191 kb on - strand, within kaiA at 1241.192 kb on + strand, within kaiA at 1241.192 kb on + strand, within kaiA at 1241.200 kb on - strand, within kaiA at 1241.205 kb on - strand, within kaiA at 1241.227 kb on - strand, within kaiA at 1241.269 kb on + strand, within kaiA at 1241.284 kb on + strand, within kaiA at 1241.288 kb on - strand, within kaiA at 1241.294 kb on - strand, within kaiA at 1241.306 kb on + strand, within kaiA at 1241.321 kb on - strand, within kaiA at 1241.350 kb on - strand at 1241.423 kb on + strand at 1241.431 kb on - strand at 1241.446 kb on + strand at 1241.480 kb on + strand at 1241.480 kb on + strand at 1241.488 kb on - strand at 1241.491 kb on - strand at 1242.306 kb on + strand, within rpl27 at 1242.366 kb on + strand at 1242.380 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 1,239,567 + kaiC Synpcc7942_1216 0.65 -1.2 1,239,567 + kaiC Synpcc7942_1216 0.65 -4.2 1,239,659 + kaiC Synpcc7942_1216 0.71 -2.0 1,239,755 + kaiC Synpcc7942_1216 0.77 -2.5 1,239,761 + kaiC Synpcc7942_1216 0.78 -3.2 1,239,770 - kaiC Synpcc7942_1216 0.78 -1.4 1,239,914 + kaiC Synpcc7942_1216 0.87 -3.5 1,240,261 + kaiB Synpcc7942_1217 0.33 +2.6 1,240,443 - +3.1 1,240,463 + +3.2 1,240,463 + +2.0 1,240,543 - +3.5 1,240,772 + kaiA Synpcc7942_1218 0.25 -2.3 1,240,827 + kaiA Synpcc7942_1218 0.32 -2.3 1,240,834 + kaiA Synpcc7942_1218 0.32 -1.3 1,240,907 + kaiA Synpcc7942_1218 0.41 -3.0 1,240,925 - kaiA Synpcc7942_1218 0.43 -1.4 1,240,934 - kaiA Synpcc7942_1218 0.44 -3.7 1,240,934 - kaiA Synpcc7942_1218 0.44 -3.0 1,240,936 - kaiA Synpcc7942_1218 0.44 -4.3 1,240,966 - kaiA Synpcc7942_1218 0.48 -2.3 1,240,972 + kaiA Synpcc7942_1218 0.49 -2.6 1,240,989 + kaiA Synpcc7942_1218 0.51 -2.1 1,241,021 - kaiA Synpcc7942_1218 0.54 -4.0 1,241,021 - kaiA Synpcc7942_1218 0.54 -3.2 1,241,048 + kaiA Synpcc7942_1218 0.57 -3.5 1,241,053 + kaiA Synpcc7942_1218 0.58 -2.6 1,241,056 - kaiA Synpcc7942_1218 0.58 -3.2 1,241,065 - kaiA Synpcc7942_1218 0.59 -2.0 1,241,065 - kaiA Synpcc7942_1218 0.59 -3.8 1,241,092 - kaiA Synpcc7942_1218 0.63 -2.4 1,241,097 + kaiA Synpcc7942_1218 0.63 -2.9 1,241,097 + kaiA Synpcc7942_1218 0.63 -2.0 1,241,100 - kaiA Synpcc7942_1218 0.64 -0.8 1,241,102 + kaiA Synpcc7942_1218 0.64 -1.8 1,241,102 + kaiA Synpcc7942_1218 0.64 -3.1 1,241,121 - kaiA Synpcc7942_1218 0.66 -2.9 1,241,155 - kaiA Synpcc7942_1218 0.70 -2.8 1,241,191 - kaiA Synpcc7942_1218 0.74 -1.7 1,241,192 + kaiA Synpcc7942_1218 0.74 -2.2 1,241,192 + kaiA Synpcc7942_1218 0.74 -3.9 1,241,200 - kaiA Synpcc7942_1218 0.75 -0.4 1,241,205 - kaiA Synpcc7942_1218 0.76 -2.5 1,241,227 - kaiA Synpcc7942_1218 0.78 -2.2 1,241,269 + kaiA Synpcc7942_1218 0.83 -1.6 1,241,284 + kaiA Synpcc7942_1218 0.85 -2.6 1,241,288 - kaiA Synpcc7942_1218 0.85 -2.0 1,241,294 - kaiA Synpcc7942_1218 0.86 -4.3 1,241,306 + kaiA Synpcc7942_1218 0.88 -3.6 1,241,321 - kaiA Synpcc7942_1218 0.89 -4.8 1,241,350 - -4.3 1,241,423 + +0.1 1,241,431 - -1.9 1,241,446 + +0.2 1,241,480 + +1.0 1,241,480 + +1.8 1,241,488 - -0.9 1,241,491 - +0.3 1,242,306 + rpl27 Synpcc7942_1220 0.90 +2.7 1,242,366 + +2.5 1,242,380 - +1.2
Or see this region's nucleotide sequence