Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ndhK and ndhJ overlap by 8 nucleotides ndhJ and Synpcc7942_1183 are separated by 166 nucleotides Synpcc7942_1183 and Synpcc7942_1184 are separated by 24 nucleotides
Synpcc7942_1181: ndhK - NADH dehydrogenase subunit B, at 1,212,095 to 1,212,802
ndhK
Synpcc7942_1182: ndhJ - NADH dehydrogenase subunit J, at 1,212,795 to 1,213,313
ndhJ
Synpcc7942_1183: Synpcc7942_1183 - hypothetical protein, at 1,213,480 to 1,214,214
_1183
Synpcc7942_1184: Synpcc7942_1184 - 6-pyruvoyl-tetrahydropterin synthase-like protein, at 1,214,239 to 1,215,141
_1184
Position (kb)
1213
1214
1215 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1212.733 kb on - strand at 1212.745 kb on - strand at 1213.349 kb on - strand at 1213.359 kb on - strand at 1213.363 kb on + strand at 1213.364 kb on + strand at 1213.387 kb on - strand at 1213.431 kb on + strand at 1213.449 kb on - strand at 1213.450 kb on - strand at 1213.467 kb on + strand at 1213.488 kb on - strand at 1213.491 kb on + strand at 1213.506 kb on - strand at 1213.524 kb on + strand at 1213.572 kb on + strand, within Synpcc7942_1183 at 1213.580 kb on - strand, within Synpcc7942_1183 at 1213.625 kb on + strand, within Synpcc7942_1183 at 1213.637 kb on + strand, within Synpcc7942_1183 at 1213.637 kb on + strand, within Synpcc7942_1183 at 1213.656 kb on + strand, within Synpcc7942_1183 at 1213.656 kb on + strand, within Synpcc7942_1183 at 1213.657 kb on - strand, within Synpcc7942_1183 at 1213.661 kb on + strand, within Synpcc7942_1183 at 1213.664 kb on - strand, within Synpcc7942_1183 at 1213.770 kb on - strand, within Synpcc7942_1183 at 1213.777 kb on - strand, within Synpcc7942_1183 at 1213.784 kb on + strand, within Synpcc7942_1183 at 1213.810 kb on + strand, within Synpcc7942_1183 at 1213.847 kb on + strand, within Synpcc7942_1183 at 1213.860 kb on - strand, within Synpcc7942_1183 at 1213.873 kb on + strand, within Synpcc7942_1183 at 1213.881 kb on - strand, within Synpcc7942_1183 at 1213.881 kb on - strand, within Synpcc7942_1183 at 1213.886 kb on + strand, within Synpcc7942_1183 at 1213.971 kb on + strand, within Synpcc7942_1183 at 1213.979 kb on - strand, within Synpcc7942_1183 at 1213.999 kb on + strand, within Synpcc7942_1183 at 1214.022 kb on + strand, within Synpcc7942_1183 at 1214.048 kb on - strand, within Synpcc7942_1183 at 1214.104 kb on - strand, within Synpcc7942_1183 at 1214.154 kb on + strand at 1214.154 kb on + strand at 1214.162 kb on - strand at 1214.169 kb on + strand at 1214.177 kb on - strand at 1214.209 kb on + strand at 1214.228 kb on + strand at 1214.235 kb on - strand at 1214.260 kb on - strand at 1214.275 kb on + strand at 1214.275 kb on + strand at 1214.275 kb on + strand at 1214.283 kb on - strand at 1214.330 kb on - strand, within Synpcc7942_1184 at 1214.411 kb on - strand, within Synpcc7942_1184 at 1214.435 kb on + strand, within Synpcc7942_1184 at 1214.649 kb on + strand, within Synpcc7942_1184 at 1214.663 kb on + strand, within Synpcc7942_1184 at 1214.671 kb on - strand, within Synpcc7942_1184 at 1214.704 kb on + strand, within Synpcc7942_1184 at 1214.706 kb on + strand, within Synpcc7942_1184 at 1214.706 kb on + strand, within Synpcc7942_1184 at 1214.728 kb on + strand, within Synpcc7942_1184 at 1214.804 kb on + strand, within Synpcc7942_1184 at 1214.819 kb on - strand, within Synpcc7942_1184 at 1214.834 kb on + strand, within Synpcc7942_1184 at 1214.865 kb on + strand, within Synpcc7942_1184 at 1214.877 kb on + strand, within Synpcc7942_1184 at 1214.877 kb on + strand, within Synpcc7942_1184 at 1214.887 kb on + strand, within Synpcc7942_1184 at 1214.895 kb on - strand, within Synpcc7942_1184 at 1214.895 kb on - strand, within Synpcc7942_1184 at 1214.934 kb on + strand, within Synpcc7942_1184 at 1214.946 kb on - strand, within Synpcc7942_1184 at 1214.968 kb on + strand, within Synpcc7942_1184 at 1215.044 kb on - strand, within Synpcc7942_1184 at 1215.044 kb on - strand, within Synpcc7942_1184 at 1215.047 kb on + strand, within Synpcc7942_1184 at 1215.086 kb on + strand at 1215.086 kb on + strand at 1215.086 kb on + strand at 1215.086 kb on + strand at 1215.086 kb on + strand at 1215.088 kb on + strand at 1215.088 kb on + strand at 1215.088 kb on + strand at 1215.094 kb on - strand at 1215.094 kb on - strand at 1215.094 kb on - strand at 1215.094 kb on - strand at 1215.100 kb on + strand at 1215.100 kb on + strand at 1215.101 kb on - strand at 1215.113 kb on - strand at 1215.115 kb on + strand at 1215.135 kb on + strand at 1215.147 kb on + strand at 1215.182 kb on + strand at 1215.192 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 1,212,733 - -2.7 1,212,745 - -2.0 1,213,349 - -1.4 1,213,359 - +0.7 1,213,363 + -1.0 1,213,364 + +0.5 1,213,387 - +0.6 1,213,431 + -0.0 1,213,449 - -0.6 1,213,450 - -2.8 1,213,467 + -1.7 1,213,488 - -1.6 1,213,491 + -0.8 1,213,506 - -1.4 1,213,524 + -0.0 1,213,572 + Synpcc7942_1183 0.13 -0.8 1,213,580 - Synpcc7942_1183 0.14 -1.6 1,213,625 + Synpcc7942_1183 0.20 -1.4 1,213,637 + Synpcc7942_1183 0.21 -0.3 1,213,637 + Synpcc7942_1183 0.21 -2.7 1,213,656 + Synpcc7942_1183 0.24 -1.9 1,213,656 + Synpcc7942_1183 0.24 -2.6 1,213,657 - Synpcc7942_1183 0.24 +0.8 1,213,661 + Synpcc7942_1183 0.25 -1.7 1,213,664 - Synpcc7942_1183 0.25 -0.9 1,213,770 - Synpcc7942_1183 0.39 -1.5 1,213,777 - Synpcc7942_1183 0.40 -2.0 1,213,784 + Synpcc7942_1183 0.41 -1.2 1,213,810 + Synpcc7942_1183 0.45 +0.3 1,213,847 + Synpcc7942_1183 0.50 -1.7 1,213,860 - Synpcc7942_1183 0.52 -0.2 1,213,873 + Synpcc7942_1183 0.53 -0.7 1,213,881 - Synpcc7942_1183 0.55 -1.4 1,213,881 - Synpcc7942_1183 0.55 -0.9 1,213,886 + Synpcc7942_1183 0.55 -1.6 1,213,971 + Synpcc7942_1183 0.67 -0.1 1,213,979 - Synpcc7942_1183 0.68 -1.0 1,213,999 + Synpcc7942_1183 0.71 -2.1 1,214,022 + Synpcc7942_1183 0.74 -1.8 1,214,048 - Synpcc7942_1183 0.77 -1.8 1,214,104 - Synpcc7942_1183 0.85 -0.2 1,214,154 + -2.2 1,214,154 + -1.3 1,214,162 - -0.2 1,214,169 + -0.7 1,214,177 - -2.0 1,214,209 + +1.8 1,214,228 + -1.1 1,214,235 - -1.8 1,214,260 - -1.2 1,214,275 + -2.3 1,214,275 + -3.7 1,214,275 + -1.9 1,214,283 - +0.8 1,214,330 - Synpcc7942_1184 0.10 -0.8 1,214,411 - Synpcc7942_1184 0.19 -0.5 1,214,435 + Synpcc7942_1184 0.22 -1.4 1,214,649 + Synpcc7942_1184 0.45 -3.1 1,214,663 + Synpcc7942_1184 0.47 -0.2 1,214,671 - Synpcc7942_1184 0.48 -1.6 1,214,704 + Synpcc7942_1184 0.51 -0.5 1,214,706 + Synpcc7942_1184 0.52 -0.9 1,214,706 + Synpcc7942_1184 0.52 -1.2 1,214,728 + Synpcc7942_1184 0.54 -0.7 1,214,804 + Synpcc7942_1184 0.63 -0.9 1,214,819 - Synpcc7942_1184 0.64 -0.5 1,214,834 + Synpcc7942_1184 0.66 -1.7 1,214,865 + Synpcc7942_1184 0.69 -0.9 1,214,877 + Synpcc7942_1184 0.71 -0.3 1,214,877 + Synpcc7942_1184 0.71 -0.3 1,214,887 + Synpcc7942_1184 0.72 -1.0 1,214,895 - Synpcc7942_1184 0.73 -0.8 1,214,895 - Synpcc7942_1184 0.73 -0.4 1,214,934 + Synpcc7942_1184 0.77 -1.9 1,214,946 - Synpcc7942_1184 0.78 -0.7 1,214,968 + Synpcc7942_1184 0.81 -0.7 1,215,044 - Synpcc7942_1184 0.89 -2.2 1,215,044 - Synpcc7942_1184 0.89 -0.2 1,215,047 + Synpcc7942_1184 0.89 -1.5 1,215,086 + -0.1 1,215,086 + +0.3 1,215,086 + -0.2 1,215,086 + -1.5 1,215,086 + -0.1 1,215,088 + -2.0 1,215,088 + -2.9 1,215,088 + -2.7 1,215,094 - -1.5 1,215,094 - -0.4 1,215,094 - -1.9 1,215,094 - -0.0 1,215,100 + -1.5 1,215,100 + -2.0 1,215,101 - +0.7 1,215,113 - -2.0 1,215,115 + -0.3 1,215,135 + +0.4 1,215,147 + +0.2 1,215,182 + +1.3 1,215,192 - -0.8
Or see this region's nucleotide sequence