Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aas and Synpcc7942_0919 are separated by 48 nucleotides Synpcc7942_0919 and psaK1 are separated by 196 nucleotides psaK1 and Synpcc7942_0921 are separated by 117 nucleotides Synpcc7942_0921 and gltX overlap by 4 nucleotides
Synpcc7942_0918: aas - long-chain-fatty-acid CoA ligase, at 924,079 to 926,028
aas
Synpcc7942_0919: Synpcc7942_0919 - hypothetical protein, at 926,077 to 926,523
_0919
Synpcc7942_0920: psaK1 - photosystem I reaction center, at 926,720 to 926,974
psaK1
Synpcc7942_0921: Synpcc7942_0921 - hypothetical protein, at 927,092 to 927,394
_0921
Synpcc7942_0922: gltX - Glutamate--tRNA ligase, at 927,391 to 928,293
gltX
Position (kb) 926
927 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 925.739 kb on + strand, within aas at 925.744 kb on + strand, within aas at 925.762 kb on - strand, within aas at 925.802 kb on - strand, within aas at 925.814 kb on + strand, within aas at 925.822 kb on - strand, within aas at 925.822 kb on - strand, within aas at 925.835 kb on + strand at 925.840 kb on + strand at 925.855 kb on - strand at 925.890 kb on + strand at 925.910 kb on + strand at 926.042 kb on + strand at 926.050 kb on - strand at 926.075 kb on + strand at 926.092 kb on + strand at 926.093 kb on - strand at 926.126 kb on - strand, within Synpcc7942_0919 at 926.186 kb on - strand, within Synpcc7942_0919 at 926.279 kb on - strand, within Synpcc7942_0919 at 926.436 kb on + strand, within Synpcc7942_0919 at 926.500 kb on - strand at 926.526 kb on - strand at 926.672 kb on + strand at 926.699 kb on + strand at 926.704 kb on + strand at 926.709 kb on + strand at 926.801 kb on + strand, within psaK1 at 926.806 kb on + strand, within psaK1 at 926.895 kb on + strand, within psaK1 at 926.933 kb on + strand, within psaK1 at 926.935 kb on + strand, within psaK1 at 927.007 kb on - strand at 927.048 kb on - strand at 927.114 kb on + strand at 927.122 kb on - strand at 927.122 kb on - strand at 927.165 kb on - strand, within Synpcc7942_0921 at 927.177 kb on - strand, within Synpcc7942_0921 at 927.177 kb on - strand, within Synpcc7942_0921 at 927.181 kb on + strand, within Synpcc7942_0921 at 927.201 kb on - strand, within Synpcc7942_0921 at 927.202 kb on + strand, within Synpcc7942_0921 at 927.275 kb on + strand, within Synpcc7942_0921 at 927.275 kb on + strand, within Synpcc7942_0921 at 927.339 kb on - strand, within Synpcc7942_0921 at 927.368 kb on - strand at 927.389 kb on - strand at 927.420 kb on + strand at 927.420 kb on + strand at 927.420 kb on + strand at 927.428 kb on + strand at 927.446 kb on + strand at 927.451 kb on + strand at 927.454 kb on - strand at 927.478 kb on + strand at 927.486 kb on - strand, within gltX at 927.508 kb on - strand, within gltX at 927.526 kb on + strand, within gltX at 927.526 kb on + strand, within gltX at 927.532 kb on + strand, within gltX at 927.540 kb on - strand, within gltX at 927.586 kb on + strand, within gltX at 927.591 kb on + strand, within gltX at 927.605 kb on + strand, within gltX at 927.605 kb on + strand, within gltX at 927.613 kb on - strand, within gltX at 927.617 kb on + strand, within gltX at 927.669 kb on + strand, within gltX at 927.670 kb on - strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand at 927.672 kb on + strand, within gltX at 927.674 kb on + strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.695 kb on + strand, within gltX at 927.695 kb on - strand, within gltX at 927.695 kb on - strand, within gltX at 927.716 kb on - strand, within gltX at 927.738 kb on - strand, within gltX at 927.738 kb on - strand, within gltX at 927.801 kb on + strand, within gltX at 927.834 kb on - strand, within gltX at 927.834 kb on - strand, within gltX at 927.903 kb on + strand, within gltX at 927.911 kb on - strand, within gltX at 927.939 kb on - strand, within gltX at 927.940 kb on + strand, within gltX at 927.973 kb on - strand, within gltX
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 925,739 + aas Synpcc7942_0918 0.85 -0.5 925,744 + aas Synpcc7942_0918 0.85 -0.4 925,762 - aas Synpcc7942_0918 0.86 -1.2 925,802 - aas Synpcc7942_0918 0.88 -1.2 925,814 + aas Synpcc7942_0918 0.89 -3.8 925,822 - aas Synpcc7942_0918 0.89 -2.3 925,822 - aas Synpcc7942_0918 0.89 -1.5 925,835 + -1.8 925,840 + -1.2 925,855 - -0.7 925,890 + -0.6 925,910 + +0.4 926,042 + +0.9 926,050 - -1.2 926,075 + +1.6 926,092 + +2.3 926,093 - +1.3 926,126 - Synpcc7942_0919 0.11 +3.3 926,186 - Synpcc7942_0919 0.24 +0.4 926,279 - Synpcc7942_0919 0.45 +2.1 926,436 + Synpcc7942_0919 0.80 +2.4 926,500 - +1.0 926,526 - +1.2 926,672 + +1.0 926,699 + -0.1 926,704 + -1.2 926,709 + -0.8 926,801 + psaK1 Synpcc7942_0920 0.32 -1.4 926,806 + psaK1 Synpcc7942_0920 0.34 +0.1 926,895 + psaK1 Synpcc7942_0920 0.69 -0.4 926,933 + psaK1 Synpcc7942_0920 0.84 -0.6 926,935 + psaK1 Synpcc7942_0920 0.84 -1.5 927,007 - -0.4 927,048 - -0.4 927,114 + +0.3 927,122 - -2.4 927,122 - +0.0 927,165 - Synpcc7942_0921 0.24 -0.3 927,177 - Synpcc7942_0921 0.28 +1.5 927,177 - Synpcc7942_0921 0.28 -1.2 927,181 + Synpcc7942_0921 0.29 -2.0 927,201 - Synpcc7942_0921 0.36 -0.4 927,202 + Synpcc7942_0921 0.36 +0.9 927,275 + Synpcc7942_0921 0.60 +1.0 927,275 + Synpcc7942_0921 0.60 +0.4 927,339 - Synpcc7942_0921 0.82 -1.1 927,368 - +1.4 927,389 - -0.5 927,420 + -0.5 927,420 + -1.2 927,420 + +0.6 927,428 + -0.8 927,446 + +0.1 927,451 + -2.7 927,454 - +0.3 927,478 + -0.3 927,486 - gltX Synpcc7942_0922 0.11 +1.5 927,508 - gltX Synpcc7942_0922 0.13 +1.9 927,526 + gltX Synpcc7942_0922 0.15 +0.6 927,526 + gltX Synpcc7942_0922 0.15 +0.2 927,532 + gltX Synpcc7942_0922 0.16 +0.5 927,540 - gltX Synpcc7942_0922 0.17 -0.2 927,586 + gltX Synpcc7942_0922 0.22 +0.0 927,591 + gltX Synpcc7942_0922 0.22 -0.2 927,605 + gltX Synpcc7942_0922 0.24 +0.7 927,605 + gltX Synpcc7942_0922 0.24 +0.2 927,613 - gltX Synpcc7942_0922 0.25 +0.9 927,617 + gltX Synpcc7942_0922 0.25 -0.9 927,669 + gltX Synpcc7942_0922 0.31 +0.1 927,670 - gltX Synpcc7942_0922 0.31 -1.1 927,672 + gltX Synpcc7942_0922 0.31 -1.2 927,672 + gltX Synpcc7942_0922 0.31 +0.7 927,672 + gltX Synpcc7942_0922 0.31 +2.4 927,672 + gltX Synpcc7942_0922 0.31 -1.6 927,672 + gltX Synpcc7942_0922 0.31 +0.8 927,672 + gltX Synpcc7942_0922 0.31 -4.0 927,672 + gltX Synpcc7942_0922 0.31 -0.1 927,672 + gltX Synpcc7942_0922 0.31 +1.0 927,672 + +1.6 927,672 + gltX Synpcc7942_0922 0.31 -1.2 927,674 + gltX Synpcc7942_0922 0.31 +0.1 927,680 - gltX Synpcc7942_0922 0.32 +0.4 927,680 - gltX Synpcc7942_0922 0.32 -0.1 927,680 - gltX Synpcc7942_0922 0.32 +0.4 927,680 - gltX Synpcc7942_0922 0.32 -1.2 927,680 - gltX Synpcc7942_0922 0.32 -0.3 927,680 - gltX Synpcc7942_0922 0.32 +0.9 927,680 - gltX Synpcc7942_0922 0.32 +0.6 927,680 - gltX Synpcc7942_0922 0.32 +0.8 927,680 - gltX Synpcc7942_0922 0.32 +0.2 927,680 - gltX Synpcc7942_0922 0.32 -0.4 927,695 + gltX Synpcc7942_0922 0.34 -2.2 927,695 - gltX Synpcc7942_0922 0.34 +0.4 927,695 - gltX Synpcc7942_0922 0.34 -0.3 927,716 - gltX Synpcc7942_0922 0.36 -0.9 927,738 - gltX Synpcc7942_0922 0.38 -1.4 927,738 - gltX Synpcc7942_0922 0.38 -0.5 927,801 + gltX Synpcc7942_0922 0.45 +0.6 927,834 - gltX Synpcc7942_0922 0.49 +0.9 927,834 - gltX Synpcc7942_0922 0.49 +1.0 927,903 + gltX Synpcc7942_0922 0.57 +0.1 927,911 - gltX Synpcc7942_0922 0.58 -0.2 927,939 - gltX Synpcc7942_0922 0.61 -0.5 927,940 + gltX Synpcc7942_0922 0.61 -0.2 927,973 - gltX Synpcc7942_0922 0.64 -0.1
Or see this region's nucleotide sequence