Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt crtE and folD are separated by 42 nucleotides folD and Synpcc7942_0778 are separated by 35 nucleotides Synpcc7942_0778 and Synpcc7942_0779 are separated by 92 nucleotides
Synpcc7942_0776: crtE - farnesyl-diphosphate synthase, at 767,721 to 768,626
crtE
Synpcc7942_0777: folD - Methenyltetrahydrofolate cyclohydrolase, at 768,669 to 769,538
folD
Synpcc7942_0778: Synpcc7942_0778 - hypothetical protein, at 769,574 to 770,038
_0778
Synpcc7942_0779: Synpcc7942_0779 - metal dependent phosphohydrolase, at 770,131 to 772,593
_0779
Position (kb) 769
770
771 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 769.541 kb on + strand at 769.549 kb on - strand at 769.594 kb on - strand at 770.006 kb on + strand at 770.006 kb on + strand at 770.006 kb on + strand at 770.020 kb on + strand at 770.032 kb on + strand at 770.032 kb on + strand at 770.032 kb on + strand at 770.038 kb on + strand at 770.040 kb on - strand at 770.040 kb on - strand at 770.040 kb on - strand at 770.040 kb on - strand at 770.040 kb on - strand at 770.043 kb on + strand at 770.051 kb on + strand at 770.051 kb on - strand at 770.051 kb on - strand at 770.059 kb on - strand at 770.074 kb on - strand at 770.077 kb on + strand at 770.077 kb on + strand at 770.085 kb on - strand at 770.087 kb on - strand at 770.092 kb on + strand at 770.110 kb on - strand at 770.126 kb on + strand at 770.126 kb on + strand at 770.134 kb on - strand at 770.134 kb on - strand at 770.134 kb on - strand at 770.148 kb on + strand at 770.156 kb on + strand at 770.190 kb on - strand at 770.231 kb on - strand at 770.289 kb on - strand at 770.289 kb on - strand at 770.346 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 769,541 + +1.2 769,549 - +0.2 769,594 - -1.1 770,006 + -0.9 770,006 + -1.1 770,006 + -2.2 770,020 + -0.1 770,032 + +0.6 770,032 + +0.1 770,032 + -0.7 770,038 + +0.4 770,040 - -1.5 770,040 - +2.0 770,040 - +0.4 770,040 - +0.8 770,040 - -1.2 770,043 + -2.4 770,051 + +0.0 770,051 - +0.5 770,051 - -0.6 770,059 - +2.5 770,074 - -1.6 770,077 + +2.0 770,077 + -0.2 770,085 - -0.1 770,087 - -1.0 770,092 + -0.1 770,110 - +0.6 770,126 + +1.7 770,126 + +2.0 770,134 - -0.2 770,134 - +3.0 770,134 - +2.5 770,148 + +0.6 770,156 + +1.2 770,190 - -0.9 770,231 - +1.1 770,289 - +2.0 770,289 - +2.5 770,346 - +1.3
Or see this region's nucleotide sequence