Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rnd and tRNA-Val are separated by 38 nucleotides tRNA-Val and rbp1 are separated by 272 nucleotides rbp1 and Synpcc7942_0680 are separated by 225 nucleotides
Synpcc7942_0678: rnd - 3'-5' exonuclease, at 672,767 to 673,402
rnd
Synpcc7942_R0038: tRNA-Val - tRNA-Val, at 673,441 to 673,512
tRNA-Val
Synpcc7942_0679: rbp1 - RNA-binding region RNP-1, at 673,785 to 674,108
rbp1
Synpcc7942_0680: Synpcc7942_0680 - C-5 sterol desaturase, at 674,334 to 675,149
_0680
Position (kb) 673
674
675 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 672.836 kb on + strand, within rnd at 672.898 kb on + strand, within rnd at 672.898 kb on + strand, within rnd at 672.911 kb on - strand, within rnd at 672.953 kb on + strand, within rnd at 672.967 kb on - strand at 672.999 kb on - strand, within rnd at 673.038 kb on + strand, within rnd at 673.046 kb on + strand, within rnd at 673.066 kb on - strand, within rnd at 673.067 kb on - strand, within rnd at 673.067 kb on - strand, within rnd at 673.117 kb on + strand, within rnd at 673.126 kb on - strand, within rnd at 673.138 kb on - strand, within rnd at 673.157 kb on - strand, within rnd at 673.159 kb on - strand, within rnd at 673.159 kb on - strand, within rnd at 673.159 kb on - strand, within rnd at 673.167 kb on + strand, within rnd at 673.177 kb on + strand, within rnd at 673.177 kb on + strand, within rnd at 673.177 kb on + strand, within rnd at 673.185 kb on - strand, within rnd at 673.202 kb on - strand, within rnd at 673.204 kb on + strand, within rnd at 673.212 kb on - strand, within rnd at 673.224 kb on + strand, within rnd at 673.224 kb on + strand, within rnd at 673.232 kb on - strand, within rnd at 673.232 kb on - strand, within rnd at 673.232 kb on - strand, within rnd at 673.232 kb on - strand, within rnd at 673.237 kb on - strand, within rnd at 673.242 kb on - strand, within rnd at 673.275 kb on - strand, within rnd at 673.303 kb on + strand, within rnd at 673.361 kb on - strand at 673.378 kb on + strand at 673.394 kb on - strand at 673.396 kb on + strand at 673.399 kb on + strand at 673.410 kb on - strand at 673.443 kb on - strand at 673.581 kb on + strand at 673.633 kb on - strand at 673.651 kb on + strand at 673.811 kb on - strand at 673.861 kb on - strand, within rbp1 at 673.862 kb on + strand, within rbp1 at 673.870 kb on - strand, within rbp1 at 673.875 kb on - strand, within rbp1 at 673.952 kb on - strand, within rbp1 at 673.975 kb on + strand, within rbp1 at 673.978 kb on - strand, within rbp1 at 674.020 kb on + strand, within rbp1 at 674.041 kb on - strand, within rbp1 at 674.116 kb on + strand at 674.124 kb on - strand at 674.125 kb on - strand at 674.184 kb on + strand at 674.535 kb on + strand, within Synpcc7942_0680 at 674.537 kb on - strand, within Synpcc7942_0680 at 674.610 kb on - strand, within Synpcc7942_0680 at 674.771 kb on - strand, within Synpcc7942_0680 at 674.792 kb on - strand, within Synpcc7942_0680 at 674.801 kb on + strand, within Synpcc7942_0680 at 674.886 kb on - strand, within Synpcc7942_0680 at 674.886 kb on - strand, within Synpcc7942_0680 at 674.922 kb on - strand, within Synpcc7942_0680 at 674.923 kb on + strand, within Synpcc7942_0680 at 674.924 kb on - strand, within Synpcc7942_0680 at 674.959 kb on - strand, within Synpcc7942_0680 at 674.960 kb on - strand, within Synpcc7942_0680 at 674.975 kb on + strand, within Synpcc7942_0680 at 675.004 kb on - strand, within Synpcc7942_0680
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 672,836 + rnd Synpcc7942_0678 0.11 +0.9 672,898 + rnd Synpcc7942_0678 0.21 +0.8 672,898 + rnd Synpcc7942_0678 0.21 -0.5 672,911 - rnd Synpcc7942_0678 0.23 +1.8 672,953 + rnd Synpcc7942_0678 0.29 +0.3 672,967 - +0.8 672,999 - rnd Synpcc7942_0678 0.36 +1.7 673,038 + rnd Synpcc7942_0678 0.43 -1.1 673,046 + rnd Synpcc7942_0678 0.44 +1.3 673,066 - rnd Synpcc7942_0678 0.47 -3.2 673,067 - rnd Synpcc7942_0678 0.47 -1.7 673,067 - rnd Synpcc7942_0678 0.47 +0.7 673,117 + rnd Synpcc7942_0678 0.55 +0.1 673,126 - rnd Synpcc7942_0678 0.56 +0.8 673,138 - rnd Synpcc7942_0678 0.58 +0.6 673,157 - rnd Synpcc7942_0678 0.61 -0.0 673,159 - rnd Synpcc7942_0678 0.62 +0.0 673,159 - rnd Synpcc7942_0678 0.62 -2.2 673,159 - rnd Synpcc7942_0678 0.62 -1.4 673,167 + rnd Synpcc7942_0678 0.63 -0.6 673,177 + rnd Synpcc7942_0678 0.64 +0.6 673,177 + rnd Synpcc7942_0678 0.64 +0.3 673,177 + rnd Synpcc7942_0678 0.64 +0.2 673,185 - rnd Synpcc7942_0678 0.66 -0.4 673,202 - rnd Synpcc7942_0678 0.68 +2.1 673,204 + rnd Synpcc7942_0678 0.69 +0.7 673,212 - rnd Synpcc7942_0678 0.70 +0.0 673,224 + rnd Synpcc7942_0678 0.72 +0.9 673,224 + rnd Synpcc7942_0678 0.72 +0.2 673,232 - rnd Synpcc7942_0678 0.73 -0.4 673,232 - rnd Synpcc7942_0678 0.73 -0.3 673,232 - rnd Synpcc7942_0678 0.73 +0.8 673,232 - rnd Synpcc7942_0678 0.73 -1.1 673,237 - rnd Synpcc7942_0678 0.74 +0.1 673,242 - rnd Synpcc7942_0678 0.75 -1.2 673,275 - rnd Synpcc7942_0678 0.80 -0.7 673,303 + rnd Synpcc7942_0678 0.84 -0.7 673,361 - +0.1 673,378 + -0.8 673,394 - -1.5 673,396 + +0.1 673,399 + +0.2 673,410 - -0.5 673,443 - +0.6 673,581 + -1.4 673,633 - -0.5 673,651 + -2.7 673,811 - -1.2 673,861 - rbp1 Synpcc7942_0679 0.23 -1.1 673,862 + rbp1 Synpcc7942_0679 0.24 -1.8 673,870 - rbp1 Synpcc7942_0679 0.26 -1.7 673,875 - rbp1 Synpcc7942_0679 0.28 -2.2 673,952 - rbp1 Synpcc7942_0679 0.52 -3.2 673,975 + rbp1 Synpcc7942_0679 0.59 -3.2 673,978 - rbp1 Synpcc7942_0679 0.60 -3.5 674,020 + rbp1 Synpcc7942_0679 0.73 -1.9 674,041 - rbp1 Synpcc7942_0679 0.79 -3.2 674,116 + +1.0 674,124 - -1.3 674,125 - -0.8 674,184 + -0.6 674,535 + Synpcc7942_0680 0.25 -0.8 674,537 - Synpcc7942_0680 0.25 -0.2 674,610 - Synpcc7942_0680 0.34 +1.6 674,771 - Synpcc7942_0680 0.54 +0.4 674,792 - Synpcc7942_0680 0.56 -0.2 674,801 + Synpcc7942_0680 0.57 -0.6 674,886 - Synpcc7942_0680 0.68 +0.1 674,886 - Synpcc7942_0680 0.68 +0.3 674,922 - Synpcc7942_0680 0.72 -0.2 674,923 + Synpcc7942_0680 0.72 -0.9 674,924 - Synpcc7942_0680 0.72 -1.1 674,959 - Synpcc7942_0680 0.77 +2.1 674,960 - Synpcc7942_0680 0.77 +0.3 674,975 + Synpcc7942_0680 0.79 -0.4 675,004 - Synpcc7942_0680 0.82 +0.1
Or see this region's nucleotide sequence